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We narrowed to 920 results for: Gene

Showing: 281 - 320 of 920 results

  1. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...Additional resources on the Addgene blog Find out what makes AAV a versatile tool for gene expression in mammals...provides the AAV replication (rep) and capsid (cap) genes. AAV replication uses the host’s polymerase, but... the pHelper plasmid which expresses adenovirus genes which help mediate AAV viral replication; and 3)...a higher number of viral particles for efficient gene transfer. I figure it’s better to have a bit extra...derived inverted terminal repeat sequences. Human gene therapy methods, 23 1, 18-28. PubMed PMID: 22428977...Resources on Addgene.org Learn how Addgene produces AAV and lentivirus Check out Addgene's virus protocols...3) the transfer plasmid which encodes a transgene of interest that’s packaged into the virus. Two to five...

  2. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...insertions, deletions, and point mutagenesis to complex assembly of multiple genes. Primer design for each type...amplified, restriction digested or even a synthesized gene. PCR is the primary method to produce the linear...included in a single PCR, for example for subcloning genes from different templates. All PCRs are run as an... 5’ end of either primer. Subcloning - To move a gene from one vector to another simply amplify the vector...protein-coding sequence, or a mutation can be introduced in a gene, while adding a GFP-tag (Figure 3). Achieving multiple... to the 5’ end of the reverse primer. Mutagenesis - Mutagenesis is performed by designing primers astride...using the same three step protocol:  Fragment generation - Any linear DNA fragments can be assembled in...

  3. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...where his group focuses on gene regulation in nematode molting and spermatogenesis. His group also builds ... one of the model organisms which I use to study gene regulation during developmental processes, CRISPR...Craig Mello’s lab – used inactivation of the unc-22 gene as their selection marker (Kim et al., 2014). Meanwhile... I was able to knock-in a 2xFLAG epitope into my gene of interest, I encountered low efficiency (0.13%...yeast cells using modified Cas9 proteins to regulate gene expression (CRISPRi/CRISPRa), visualize specific...Gaul U, Forstemann K (2014) Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous...CRISPR toolbox for C. elegans: Cpf1 as an alternative gene editing system for AT-rich sequences https://doi.org...

  4. Introducing the Addgene Antibodies 101 eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2024, 1:15 p.m.
    ...Addgene is very excited to share the very first edition of our Antibodies 101 eBook! The latest addition... always, don’t hesitate to reach out to help@addgene.org with any thoughts, questions, or feedback. Happy...

  5. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...number of CRISPR libraries Addgene has distributed (1C), from 2012 to 2023. Addgene’s collection of CRISPR ...As we celebrate twenty years at Addgene, we are sharing stories of our repository’s positive impact on...community. They did this by depositing them in Addgene’s repository. Other researchers, eager to try this... tools of their own to share. Over the years, Addgene’s repository has grown to include many CRISPR tools...This growth is supported by new labs joining the Addgene CRISPR community, with approximately 100 new CRISPR...CRISPR depositors each year.    Figure 1: Addgene CRISPR distribution and deposits. (A) Cumulative... (B) Cumulative CRISPR plasmids deposited with Addgene by year. (C) Cumulative number of laboratories ...

  6. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...experiments directly from Addgene. How Addgene is Reimagining Antibody Sharing Addgene is building our antibody...antibodies via Addgene to maximize the reach and impact of your work. Please email deposit@addgene.org if you ...Antibodies and Grow From There Addgene’s antibody resource is funded by a generous grant from the NIH’s Brain...reagents, share your plasmids via Addgene! Email deposit@addgene.org to learn more or get started.  If...Addgene is thrilled to announce the launch of our latest community resource: Recombinant Antibodies! ... your experiments. To promote reproducibility, Addgene will have unique Research Resource Identifiers ... preparations (Bradbury et al., 2015). All of Addgene’s antibodies are plasmid-based and recombinant. ...

  7. Participate in the Pets of Addgene Scavenger Hunt

    Type
    Blog Post
    Published
    April 1, 2021, 1:15 p.m.
    ...You will be trying to find pictures of Addgene pets with Addgene-branded clothing on the blog. Below each...just fun and games this April Fool’s Day on the Addgene blog.  We’ve been mostly working remotely for the.... Now, we’ve collected photos of our pets with Addgene gear, and have unleashed them all over our recently...

  8. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    Published
    Jan. 25, 2021, 5:54 p.m.
    ... also find information on using Addgene’s website, the latest Addgene news, and awards that we offer there...Say hello to the new Addgene blog! Same URL, same great articles to help you with your experiments, but...articles you want to find without even leaving the Addgene blog! Just click on the search bar at the top of...interview. Get in touch by emailing us at blog@addgene.org or tell us in the comments below. We’ll track...Be the first to know about new articles on the Addgene blog by subscribing to instant alerts below.   ...

  9. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    Published
    Aug. 13, 2020, 1:21 p.m.
    ...their roles at Addgene. I had a great time speaking with Quintin Marcelino who is Addgene’s Science Video...change. Having been at Addgene for a month, I can say with certainty that Addgene is a company that cares...Sociology and Dance at Skidmore College. I was also Addgene’s Marketing Intern this summer! With the help of...made me intrigued by the marketing internship at Addgene. Although my interests are very clear, I had no...COVID was getting, I was hoping to walk into the Addgene office my first day and meet everyone but this ...Marketing Intern, I was tasked with continuing Addgene’s Meet an Addgenie Series on Instagram. I interviewed...project, I observed what it was like to work at Addgene. This is exemplified in my work interviewing fellow...

  10. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...all Addgene news Resources on Addgene.org Check out our job postings Learn more about Addgene's mission...about Addgene’s software development team and how we work.  Additional resources on the Addgene blog ...When you think of Addgene, chances are software development is not one of the things that comes to mind... is a lot of software development happening at Addgene under the hood. A significant portion of our development... benefits. We support everyone that works with Addgene in some way. For users, we just released a major...viral orders are being fulfilled more quickly. As Addgene grows and evolves, we will be here to increase ...development team and the development process at Addgene. Diversity supports our innovation Our software...

  11. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...Communications Manager Addgene's book club Indeed, reading is ingrained into Addgene’s culture. We have a ...still going on today! Addgene Book Club (ABC) founder, Eric Perkins, is Addgene’s Director of Science Product... of Addgene’s culture. Have you read any of these books? Whether you have or not, let the Addgene community...Additional resources on the Addgene blog Learn about company culture at Addgene Read about other lunch activities...From Addgene Book Club to our on-site book swap/library, reading is part of the culture here at Addgene...and explains the origins of ABC: I founded the Addgene Book Club (aka ABC) in 2011 after having numerous... activities at Addgene ...

  12. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ...blog@addgene.org.  Additional Resources on the Addgene Blog New Educational Resource: The Addgene Videos...Addgene has distributed over 800,000 plasmids since 2004. In that time, our lab has gotten a lot experience...decided to share some of our tips in our new series: Addgene Lab Tips! In each short episode of this series...Science: We Share Science Additional Resources on Addgene.org Browse our Video Library Check out Our Protocol...

  13. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...Management. Professor Feng Zhang’s original 2013 gene editing paper on CRISPR/Cas amassed nearly 2,400...deposited the associated plasmids with Addgene. Since then, Addgene has filled over 6,500 requests for these...associated with Addgene deposits accumulate more citations than those without Addgene deposits? Even more...with Addgene causes a paper to get cited more?   Articles with and without plasmids at Addgene To ...authors deposited with Addgene and those without plasmids deposited with Addgene. Together these journals...of which had plasmids deposited with Addgene. Articles with Addgene plasmids get more citations So what... at Addgene – typically about four times as many as papers without plasmids deposited with Addgene (Figure...

  14. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    Published
    Jan. 11, 2024, 2:15 p.m.
    ...media, you can find us on Bluesky: @addgene.bsky.social; or @addgene on Instagram, TikTok, Facebook, and...we will not delete @addgene on X. This will additionally ensure that the @addgene handle is always associated...an official Addgene account. You can reach out to us directly via email at help@addgene.org, and we invite...Addgene is announcing we have chosen to stop all activity on X, formerly Twitter. If you are interested...mainstay of conversation in the scientific community. Addgene has been considering a gradual move off X as a ...to paid content regardless of user preference. Addgene is a values-driven nonprofit. Recent remarks and...posting, monitoring, and resharing content on X.  Addgene deeply values our community and recognizes the ...

  15. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ... Joining the Addgene crew!  This pattern was consistent: everyone I spoke with at Addgene, no matter their...hunting and interviews, I accepted a position at Addgene as a marketing and social media co-op. There are...realized I had made the right decision to choose Addgene for my co-op, but I know it didn’t take long. It... It may have been at one of my first “All Addgene” meetings, when Melina, our CSO and co-founder, approached...analytics, something that had never been done at Addgene before. This meant I was responsible for establishing...strategy. I really believe that the culture at Addgene cultivates trust between coworkers and that trust...retrospect, nonsensical. I have found the people at Addgene have become my friends, mentors, and some of my...

  16. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...from electrical activity to gene expression, and even CRISPR based gene editing. But, how do you decide ...cells expressing the optogenetic tool causing a change in cellular activity or gene expression. Experimental...activating the optogenetic tool, it can also cause problems when using multiple optogenetics or biosensors...information on hardware, optogenetics tools, viruses, and more.  Conclusion   Optogenetic tools provide a powerful...! Since then, scientists have developed many optogenetic tools to control cellular activity with exquisite...exquisite precision using only light. Optogenetics, or light activated tools, can control everything from... post, we will discuss some broad classes of optogenetic tools, and what factors to consider when choosing...

  17. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    Published
    July 7, 2016, 2:30 p.m.
    ...raffled off one of our highly sought-after "nice genes…" tshirts, and the lucky winner was Shagun Sharma...Developing the Addgene microbiology community #ASMMicrobe2016 was a great opportunity for Addgene to let microbiologists...materials! Resources on the Addgene Blog Learn How You Can Use Addgene's Microbiology Pages Read Our ...The start of the conference After setting up the Addgene booth on Thursday evening, I stayed to see the ...with lots of researchers who weren’t aware of Addgene’s plasmid sharing services, as well as #happydepositors...dedicated to science communication through art. Addgene Advisory Board member Siouxsie Wiles gave us a ... about amazing work already represented in the Addgene repository (like these new Rainbow Bacterial Fluorescence...

  18. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ... in most cases at Addgene, we check what we call the “insert,” (the gene or genetic element cloned into...information for a given plasmid are the backbone, inserted gene/functional mutations, antibiotic resistance, restriction... Plasmid Learn How Addgene Uses Barcodes to Track Samples Resources on the Addgene Website View our ...the best things about sharing plasmids through Addgene is that we provide an added level of confidence... plasmid quality is to stay organized. Here at Addgene, we collect data on every plasmid we distribute...examples include Benchling, Serial Cloner, and Snapgene), and save these to a shared computer or server...physical sample and its associated information are at Addgene, we are ready to sequence verify the plasmids. ...

  19. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ... researchers with many different ways to control gene expression. Get the full scoop from Addgenie A. ...how Cas9 homolog, Cpf1, may make it easier to make gene edits at new genomic locations. 6. CRISPR 101: Non-homologous...Adeno-associated viral (AAV) vectors are useful for in vivo gene delivery, but have limited packaging capacity making...2015 has been an exciting year for the Addgene blog. We’ve released over 80 posts on a diverse array ...have started providing recorded interviews with Addgene community members. In addition we’ve experienced...them a read if you’ve missed them! Breakdown of Addgene's top blog posts from 2015 Our most popular posts...your own guest posts by e-mailing us at blog@addgene.org. Thank you again for a fantastic year and happy...

  20. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    Published
    Nov. 13, 2015, 3:30 p.m.
    ...is in this subject.” Outreach travels with Blugene Blugene loves to visit researchers, so Outreach Scientist...Regional High School Resources at Addgene  Read more Inside Addgene Blog Posts Get Career Advice from...their day with breakfast and an introduction to Addgene with our Executive Director, Joanne Kamens. As ...to start networking! Learning about careers at Addgene Afterwards, the students split into two groups ...groups for an in-depth look at some of the teams at Addgene. An introduction to plasmids with Director of Biology...slideshow from our UK team member Benoit Giquel. Bluegene’s globetrotting impressed our guests, as did how...with the “comfortable surroundings” of the new Addgene office space.  Thanks Abigail, we think it’s pretty...

  21. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...this is to examine expression of the early response gene c-fos. For example, using Chr2 stimulation should...and standardization of genetic tools in the laboratory mouse, most optogenetics is performed in this species...Central PMCID: PMC3032981. Resources at Addgene A Primer on Optogenetics: Introduction and Opsin Delivery The...considerations you should think about when developing optogenetics behavioral experiments. There are far too many...many behaviors that have utilized optogenetics to be fully summarized in a short blog post, but some examples...ill-defined region of the brain. With the advent of optogenetics, ICSS can now be done with neuronal cell-type...the literature to find all the applications of optogenetics relevant to your field. Ultimately, your question...

  22. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ... development of optogenetics, has genetically manipulated a number of the opsin genes to alter the light...have also been engineered to express various opsin genes [7-9]. The advantage of using these lines is, if...Regardless of the method of expression of your opsin gene, it is essential to test the expression of your ...PMID: 22470147.  6. Gore BB, et al. Manipulating gene expression in projection-specific neuronal populations...sections of optogenetics papers. Optogenetics step one: Deciding how you want to use optogenetics The most...things in science, optogenetics is best used to address a specific question. Optogenetics is a tool and, ...electrophysiology to prove that your optogenetic manipulations are generating action potentials (or lack there...

  23. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ...Hey guys, Blugene here! As Addgene’s mascot, I’ve watched Addgene grow into the successful, internationally...to the entire Addgene family; the depositors and requesting scientists, all of the Addgene employees, the... the Addgene board members, vendors, Addgene supporters, and of course, Executive Director, Joanne Kamens...week, the Addgene 10-year Anniversary Gala celebrated all of the people who have helped Addgene grow and...changes at Addgene over the last 10 years as well as the many people that make up the Addgene family. It...Ken Fan each spoke about what Addgene means to them. Ken mentioned Addgene’s incredible growth and our determination...make copies of the newspaper, Addgene uses E. coli bacteria to generate copies of plasmids. The community...

  24. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...ribosome binding site (RBS) followed by a reporter gene. The hairpin blocks translation by sequestering ...reveals the RBS and allows translation of the reporter gene resulting in GFP signal. With the addition of standards...antibiotic resistance markers, promoters, and inducible gene expression systems in bee gut microbiome species...or preparation, and deliver CRISPR components for gene disruption or CRISPRi. While designed to study the...address these questions, biologists often use next-generation sequencing. Sequencing the 16S rRNA reveals the... also study the microbiome by using engineered genetic tools and reporter microbes. Let’s take a look ...microbiome with sequencing is vast, the ability to genetically alter microbiome members is limited and many ...

  25. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    .... Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression from an...pooled CRISPR knockout libraries) and individual gene knockouts (using gRNA lentiviruses). Separating ...performing a screen or even a single gene knockout. Top AAV: pAAV-hSyn-DIO-hM4D(Gi)-mCherry Our current...adult rat brain depending on the transduced area. Gene Ther. 2003 Feb;10(4):337-47. PubMed PMID: 12595892...Resources on Addgene.org Find Ready-to-Use Lentivirus Find Ready-to-Use AAV Catch up on Chemogenetics ...accelerate their work. After all, as we like to sayat Addgene, productivity is infectious. Curious which viruses...please)... Top Lentivirus: lentiCas9-Blast Most of Addgene’s lentiviral inventory is based on CRISPR tools,...

  26. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    Published
    Dec. 5, 2023, 2:15 p.m.
    ... PellettieriNew Acoustic Reporter Genes: Ultrasound Imaging of Gene ExpressionAuthors: Marjorie Buss and..., and that means it's officially the start of Addgene's winter holiday celebrations! We're kicking it ...Joachim Goedhart  Created in collaboration with Addgene writers High Complexity Golden Gate Assembly with...DADExperts: Matthew Norton and NEBGCE4All: Making Genetic Code Expansion AccessibleResearcher: Ryan Mehl;...big thank you to all who have contributed to the Addgene blog this year! Your contributions have helped ...

  27. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ... your antibody is validated! Genetic Strategies This approach uses a gene knock-down or knock-out approach...all sample types are amenable to genetic manipulation, nor can all genes be easily knocked down or out. ...bioinformatician) to look at RNA expression of your gene of interest across a set of human tissues.  From...Conveniently, you found a cell line in which the gene for your protein has been mutated using CRISPR/Cas9...plasmid encoding your protein of interest, known as Gene of Interest (GOI), and transfect it into your favorite...this protein, which we will call Negative Control Gene (NCG) (A). Then you lyse the cells and run a Western... post!  Resources on Addgene.org Find plasmids encoding antibodies at Addgene Stay tuned for ready-to-use...

  28. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...Resources on Addgene.org Visit Our Microbiology Pages Use the FX Cloning Sytem to Clone Your Gene of Interest...making it difficult to quickly identify possible gene function. In this post, I will describe a useful...done, this technique can also help pinpoint the genes within a sequenced genome that encode for functional...digesting phage genomic DNA into 1-3 kb (approximately gene-sized) fragments so that these fragments can be ...results in optimal likelihood of capturing a whole RBP gene without causing issues associated with cloning large...back to the original plate to identify which phage gene encodes that protein! First, block your membranes...for a review, see Simpson et al., 2015). Visit Addgene's MIcrobiology Pages The problem: Host binding proteins...

  29. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    .... Current table columns are target gene, species, sequence, Addgene plasmid ID (if we have one associated...reviewed articles. If you’re targeting your favorite gene with CRISPR, using one of these validated gRNAs ...is worth trying alternate names, particularly for gene names. If you find a sequence that will work for... planning an experiment to performing one.  At Addgene, over 120 labs have deposited CRISPR reagents, ...already been shown to work in CRISPR experiments. Addgene’s dedication to resource sharing has led us to develop... is welcomed for validated gRNAs that are not generated from a plasmid. If you, for example, prefer to...Sharing. 2016. In press. Additional Resources on the Addgene Blog Learn How to Design Your gRNA Use the CRISPR...

  30. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...Subscribe to the Addgene newsletter for updates on all things Addgene; or subscribe to the Addgene blog for updates...request an AAV composed of an AAV plasmid in the Addgene repository and their choice of five different serotypes...comes to ordering high-quality materials from the Addgene repository. Read on to learn more.  What will be... be available?  Over 3000 AAV plasmids in the Addgene repository will be available for Packaged on Request...request. Request details can be accessed within your Addgene account. Preps will be available for request in...Once submitted, the request will be reviewed by Addgene for legal approvals and technical compatibility...Cart” button will appear with the request in your Addgene account.  Orders do not need to be placed immediately...

  31. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    Published
    Feb. 27, 2020, 2:15 p.m.
    ... is easy. Ordering genes on plasmids online is a little trickier -- but at Addgene, we try to make the...here at Addgene. If you’ve called our customer service line or sent an email to help@addgene.org, you’ve...seller. However, Addgene is a bit different from other vendors. As a nonprofit, Addgene does not own the... bigger question: How does a customer place an Addgene order using a PO number? Why do I have to order...distribute it on behalf of depositing institutions. Addgene cannot fulfill orders based on PO documents that...information that is entered at checkout is used to generate the legal agreement (MTA), which streamlines the... with the recipient organization can create an Addgene account and place an order: a purchasing agent,...

  32. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ...Welcome to Addgene, Aavery!       Original post: In December, we introduced you to Blugene’s new virus...March 6, 2020. After months of input and polling, Blugene's new virus friend finally has a name! We thank ...we’re excited to see your thoughts. Our mascot Blugene can’t wait either. Acknowledgements We would like...

  33. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    Published
    July 16, 2019, 12:59 p.m.
    ...throughput studies of optogenetics.  Common opsins used in zebrafish Optogenetics functions through opsins....  Additional resources on the Addgene blog Find all our optogenetics blog posts Read blog posts about... viral vectors Subscribe to the Addgene blog Resources on Addgene.org Learn about our ready-to-use viral...Optogenetics, the use of light sensitive proteins (opsins) to manipulate cell activity, enables researchers...escape reflex. These traits, coupled with short generation times and high fecundity, make zebrafish ideal...are light sensitive proteins. Opsins used in optogenetic studies are not native to vertebrates and are...swimming  Researchers at the Isacoff lab applied optogenetics in zebrafish larvae to identify new neurons ...

  34. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    Published
    June 13, 2019, 12:19 p.m.
    ...content from Addgene, subscribe to Addgene’s YouTube Channel. Additional resources on the Addgene blog Learn...about Addgene's Lab Tips video series Resources on Addgene.org Find lab protocols on the Addgene website...Since rolling out our viral service in 2016, Addgene has shipped thousands of AAV and lentiviral preps... purity of all AAV viral lots. Aligning with Addgene’s mission to “accelerate research and discovery ...improve your experiment results! Subscribe to the Addgene YouTube page! AAV purification by iodixanol gradient...calculations on how to prepare a plasmid stock used to generate a standard curve, visit the AAV Titration by qPCR...website Watch Addgene's video collection ...

  35. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    Published
    June 16, 2017, 2 p.m.
    .... Along with our role as a plasmid repository, Addgene aims to be a vital educational resource for scientists...mind, we are very excited to debut our brand new Addgene Videos page! Videos are a powerful tool for communicating...or to answer any questions you might have about Addgene. The page is divided into three main sections: ... real Addgenies who have a variety of roles at Addgene.  You’ll hear all about their career paths, future...feature screen-capture footage to help you navigate Addgene’s vast online resources. Our most popular How-To...stage of the selection and checkout process on Addgene’s website: We’ll be continuously updating this...comments section below! Additional Resources on the Addgene Blog 5 Science Rap Videos and Their Creator: Tom...

  36. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...their mission. Addgene is fortunate in that we are able to fulfill our mission by generating revenue through... is a bit misleading. Nonprofits can generate revenue (as Addgene does through materials distribution)... at Addgene If you’re ever curious what specifically drives the decision making process at Addgene, just...in the community Addgene fosters a culture of respect, collaboration, and fun Addgene believes there is... on the Addgene Blog Learn about Our Collaboration with Fellow Nonprofit Seeding Labs Addgene Supports...to Our Interview with Addgene Board Member, Connie Cepko Resources on Addgene.org Learn More About Our...Our Mission Meet the Addgene Leadership Team Get the Latest News from Addgene ...

  37. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ... time or location can be critical when, say, the gene is essential during development and a knockout is...the Addgene blog Viral Vectors 101: Optogenetics Additional resources on Addgene.org Optogenetics Guide... The Chow Lab has also generated a variety of membrane-recruitment optogenetic tools for manipulating ...a full toolbox and workflow for generating new Rho GTPase optogenetic systems (Berlew et al., 2022). Stopping...LOV, CRY2-CIB, and other optogenetic modules? Or want to find more optogenetic tools in our collection?...collection? Check out our Optogenetics Guide or browse our collections of optogenetics plasmids – and keep an eye...Optogenetics gives you the power to control cells or organisms with the flip of a switch. You may be ...

  38. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...a second plasmid will be needed to introduce the gene of interest containing the chosen codon that will...limiting. It may be time, then, to look towards genetic code expansion (GCE): techniques in which non-canonical...essentially those needed to make site-directed mutagenesis, many researchers new to GCE aren’t able to successfully...added phosphoserine. A community resource Like Addgene, GCE4All is a community resource which depends ...researchers. One way of doing that is by using Addgene’s Blue Flame award to identify highly requested ...the plasmids and bacterial strains (available at Addgene for GCE work. Plus, keep an eye on our blog – we...., Griffith, M. C., & Schultz, P. G. (1989). A General Method for Site-specific Incorporation of Unnatural...

  39. Addgene’s First AAV Data Hub Challenge

    Type
    Blog Post
    Published
    Sept. 20, 2022, 1:15 p.m.
    ...We're going virtual for Addgene’s first AAV Data Hub Challenge, now taking place Dec. 6, 2022! Share ...Anyone can submit data from their CLOVER lab or Addgene AAV experiments to the Data Hub. Submit your data...travel award. All participants will receive an Addgene t-shirt.  Our judges, Drs. Connie Cepko, Ian Wickersham...

  40. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ...to highlight some of the top articles from the Addgene blog this year. Spoiler alert: many of the top ...subset of the fluorescent protein family and they generate color! They've been adapted for many molecular...SARS-CoV-2, including many with plasmids deposited at Addgene! 2. DNA Purification Without a Kit by Beth Kenkel...
Showing: 281 - 320 of 920 results