We narrowed to 318 results for: Mut

...2006) Retrograde neuronal tracing with a deletion-mutant rabies virus. Nat Methods 4:47–49 . https://doi.org...

...) and GRAB_NE1m (medium affinity), as well as GRAB_NEmut as a negative control. Find the sensors at Addgene...

...plasmid replication control that are not necessarily mutually exclusive. In the first, the replication initiation...

...leverage another organization’s expertise to our mutual benefit.  Our partnership with SnapGene, for example...

... This is to avoid the chances of insertional mutagenesis, which is the integration of transgenes into ...

...everyone has a voice and the group can come to a mutual agreement is an amazing thing to be a part of and...

...the researcher, Zeocin has been shown to cause mutations even in cells with the resistance gene, so it ...

...for viral production are propagated in the endA-mutated NEB Stable strain of E. coli. In addition, plasmids...

...transduction of cells, resulting in cell population mutated by the viral pooled library. These cells can then...

...upregulation of essentially any gene by fusing mutants of Cas9 deficient in DNA cutting activity to a ...

...between cells lines, as this can be dependent on the mutation rate of the cell line, but it is broadly recommended...

...fluorescent proteins and were re-engineered by mutating cysteine residues and N-glycosylation consensus...

... promoter with somewhat weak expression. lacIq mutation increases expression of the repressor 10x, thus...

...the architecture of my cells,” or “How does this mutation impact the types of cells found in my tissue.”...

...expression in strains of bacteria that are redox mutants so they have a more oxidizing cytoplasm. expression...

...molecular tools, such as gene manipulation and mutagenesis techniques, are available to the scientific community...

... fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins." Proceedings of the...

...Corinna, et al. "Mouse cutaneous melanoma induced by mutant BRaf arises from expansion and dedifferentiation...

...s group created Spinach2 using site-directed mutagenesis of Spinach. In doing so, they identified the ...

... to knock down genes with potentially harmful mutations in therapeutic settings. Silencing of a target...

...damage other cellular structures, and genetic mutations are not easily reversible. Late last year, the...

...process makes it easy to generate wild type and mutant constructs at the same time, rather than sequentially...

...designed and screened a large library of iGluSnFR3 mutants, identifying the two  best-performing variants ...

...DH5α, HB101, NEB Stable, Stbl3, etc., are recA- mutant strains, and therefore should have reduced frequencies...

...outcome for a specific combination of genetic mutations. This is a particular challenge in pancreatic ...

...molecularly evolved from mNeptune2 through 33 mutations and 2 deletions. CyOFP1 has a higher quantum yield...

...year isolating pheromones from various C. elegans mutants by myself, I would have slowly gone crazy. As it...

...samples are different from each other, or if every permutation of pairs within the five is different. To identify...

...gene is also useful in case the gene has a weird mutation that you didn’t know about (which could affect...

... design, remember to incorporate PAM-blocking mutations (e.g. convert the NGG PAM to NGC in your repair...

...the Vancouver SciCATs. Follow her on twitter @Point_Mutation, and @SciCATSyvr. Additional Resources on ...

...epitope of the target protein. However, small mutations in the DNA of the hybridoma can occur, known as...

...of 8× Nicotiana Benthamiana and 12× Arabidopsis Mutants.” The Plant Journal, vol. 106, no. 1, Wiley, 25...

...are two main experimental avenues (that are not mutually exclusive) commonly used in optogenetics: 1) electrophysiology...

...repair (BER), the cellular process that catches mutated U’s in DNA and ideally converts them back to Cs...

...base pairs 18 and 20.   Pro tip! The Taylor lab mutated the residues in this loop to produce SuperFi-Cas9...

... action and application of Scorpion primers to mutation detection, Nucleic Acids Research, Volume 28, ...

...fluorescent marker. Injection into a conditional mutant mouse brain that expressed Cre recombinase only...