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  2. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...sustained by the availability of this strain from The Jackson Laboratory. Originally developed and characterized...this mouse strain is regaining popularity. The Jackson Laboratory, a nonprofit research institution, has...

  3. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...Taking Care of Your Behavioral Health. Work on personal development Take the myIDP (individual development...a challenging period for everyone for numerous reasons, and you shouldn’t expect yourself to be as productive...

  4. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...and some home-made equipment. It was humble in comparison to the labs you’d find in Kendall square, but...at BosLab Not all of our members start out with personal projects in mind. Thus, to encourage more involvement...

  5. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ... those at the molecular and cellular level. The reason for this is cost and complexity – anyone who works...curriculum to see if any of the activities fit your lesson plans. If you are an instructor at the college ...

  6. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    .... The problem is…how can you make a “hands-on” lesson on genetic manipulation of human stem cells when...clearer ideas about gene therapy in the following lessons. And let me tell you more, they had brilliant ideas...

  7. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...treatment. These responses vary depending on the person. However, in silico and in vitro assessment of ..., M. K., Deshpande, M., Yang, J., Reynolds, H., Bryson, C., Fogg, M., Baker, M. P., Herskovitz, J., Goletz...

  8. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...higher Cas9-independent off-target editing in comparison with single-site editing. Encouraged by the results...#186715) and hCtRNA_VT (#186716).   Fig. 2: Comparison of previous representative dual-gRNA architectures...

  9. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...Project Meetings: You do not have to include every person who could potentially be impacted by a project....team meetings, provide agenda sections for each person to add a bullet or two about what they are working...

  10. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...." She also recommends to seek out advice from seasoned PIs who can offer support. “It is an exciting ...Email. Nothing prepared me for what a PI without a personal assistant has to deal with. My inbox currently...

  11. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...For safety and production reasons, multiple plasmids with many components are used to package lab grown...will discuss them together. Gene expression The reason for most viral experiments is to deliver a genetic...

  13. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    Published
    Jan. 21, 2025, 2:15 p.m.
    ...disengagement. By tailoring my leadership approach to each person, I was able to create a more productive and cohesive...similar transitions can provide you with insights, lessons from their experiences, and practical advice for...

  15. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...mesophilic systems are the psychrophilic enzymes.  The reason behind this isn’t really hard to guess. Think about...for all the Indian iGEM teams to interact with personnel from the industry and brainstorm with each other...

  17. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...started working in a new lab and I was the only person in the lab. When I saw a request for an Addgene...scientific conference, whether you are attending in person or monitoring that conference via social media....

  18. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...precision genome editing workflow. Digital PCR comparison to other validation methods As mentioned, these...PMC3773023. 7. Vouillot, L., Thélie, A. & Pollet, N. Comparison of T7E1 and surveyor mismatch cleavage assays...

  19. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...fluorescent proteins, how to do a head-to-head comparison of fluorescent proteins and how to make a well-informed...application. In figure 2 we provide an example of the comparison of the practical brightness of two orange fluorescent...

  20. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...classification system, CasID, to help facilitate direct comparison of different enzymes. CasPEDIA is meant to be...isn’t always the winner!).   Fig. 3 – Comparison of different Cas enzymes using CasPEDIA. Adapted...

  21. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ...talk. That moment taught me an incredibly valuable lesson: Your presentation will be better if you take time... Rachel Leeson...

  22. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered how long it takes to make...if budget is a concern. The faster methods, like Gibson assembly, are typically more expensive, so you’...

  23. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...entirely new affinity reagent.    Fig. 1: A comparison of an antibody (left) to several alternative ...  Rachel Leeson...

  25. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...molecular biology and cloning, since they have reasonable introductions to lots of molecular biology,” ... Rachel Leeson...

  26. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...world of research affinity reagents, and for good reason. However, sometimes you want a tool that is a little...Variable (scFv). Size: ~25 kDa.   Figure 2: Comparison between a full IgG antibody and common antigen...

  27. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...leads with excellence. They are one of the main reasons I have so few worries and can often focus on the... reflect this diversity, remarkably so for a 90 person company. We were and remain ahead of most companies...

  28. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...cracks. In this post we’ll highlight some of the lessons we’ve learned over the years with the hope that...interesting biotechnology code.There are valid technical reasons to choose a non-Python stack, but if you're working...

  29. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...that you will be using. If you do a head-to-head comparison right, it will be valuable information for the... are engineered can be shared to simplify the comparison by others. Together, we can build a valuable ...

  30. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...fluorescent protein can be identified by a head-to-head comparison of several fluorescent proteins of the same spectral.... In figure 1 we provide an example of such a comparison between cyan fluorescent proteins using widefield...

  32. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...not at all obvious, intuitive, or logical to a layperson. When you’re communicating ideas that you think... Rachel Leeson...

  33. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...cloning methods. Exonuclease-based methods like Gibson assembly require 20-40 bp of homology at the ends...using an alternative method like Gateway cloning or Gibson assembly. Another important consideration is the...

  34. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...making them available to all researchers for a reasonable fee. We at NURSA recognize the importance of ...at www.nursa.org. Bert O’Malley, M.D., is Tom Thompson Professor and Chair of the Department of Molecular...

  36. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ... Maya Peters Kostman in May 2020 and updated by Jason Nasse in July 2023.  References and Resources References...Ramirez M, Hu X, Galvan A, Basu J, Michaelides M, Sternson S (2019) Ultrapotent chemogenetics for research...

  37. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...antibodies recognize some rat primary antibodies (Erickson 1993). It is always better to choose primary antibodies...Publications. https://doi.org/10.1177/002215549804600813 Erickson, P. A., Lewis, G. P., & Fisher, S. K. (1993). ...

  38. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...consists of highly useful vectors compatible with Gibson and Saptrap cloning systems to generate AID* tagged...degradation.      New from the viral service By Jason Nasse We regularly add new viral aliquots from our...

  39. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...and Casɸ-3. This system is notable for several reasons. Casɸ is only ~70kDa, with a molecular weight roughly...the library here New from the viral service By Jason Nasse We regularly add new viral aliquots from our...

  40. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...deficiency, Cystic fibrosis, Muscular Dystrophy, Parkinson’s disease, and HIV. A notable landmark study showed...20463753. PubMed Central PMCID: PMC3152950. 3. Jacobson, Samuel G., et al. "Improvement and decline in...
Showing: 281 - 320 of 625 results