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We narrowed to 608 results for: Sis

Showing: 281 - 320 of 608 results

  1. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...and undigested samples of the clones on an electrophoresis gel in order to differentiate which clone had...Once we had gotten our results back from the electrophoresis, my manager and I were unsure if there were...

  2. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ...  Joining the Addgene crew!  This pattern was consistent: everyone I spoke with at Addgene, no matter ...presentation.  My first time in the lab! I got to assist production on a protocol video. Don't worry - no...

  3. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...microtubules. Tanenbaum et al. then tested their hypothesis that the lower expression levels of SunTag constructs...suitability in your experiments on a protein-by-protein basis. However, since many of Addgene’s SunTag plasmids...

  5. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...detection of the tagged protein. These peptides consist of as little as 8 or as many as several hundred...10.1007/s00253-002-1158-6 Baker, M., Reproducibility crisis: Blame it on the antibodies. Nature (2015) 521,...

  7. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ...neuronal circuit tracing.     Marcus Leiwe is an assistant professor at Kyushu University. He has a particular...resolution using chemical cocktails and computational analysis." Cell 157.3 (2014): 726-739. PubMed PMID: 24746791...

  8. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ... dynamic process which involves autophagosome synthesis, delivery of materials to be degraded to the lysosome...mammalian dual-emission autophagy biosensor. Its resistance to lysosomal proteases is key to its pH-dependent...

  10. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ..., 2020. Now that we know all about antibiotic resistance genes, let’s consider another basic element of...for single- and multiple-unnatural amino acid mutagenesis in Escherichia coli. Biochemistry. 2013 Mar 12...

  11. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...perception of head injury risk. In completing a master’s thesis in science communication, I had the chance to carry...necessary to inform the experimental aspect of my thesis: a survey instrument designed to measure how individuals...

  12. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...Instead, it targets a specific audience - giving consistency, purpose, structure, and intent to the messaging...your audience, and their expectations of you, consistent and clear.   And now that we’re all on the same...

  13. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...degree and some marketing experience, with little emphasis placed on the marketing or business background...thought they would focus on “Has this person been consistent throughout their career?”, "Have they sought ...

  14. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ...activity to start a discussion. The Forced Choice Analysis is a fun example and you can find it in our Mentoring...series of mentoring blog posts, the "Forced Choice Analysis Activity", the "Peer Mentor Groups Guide", "Tips...

  15. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...beginning makes the sharing process smoother, more consistent, and more pleasant for everyone, which in turn...including MTAs, is perfect, but it does provide consistency and transparency in an otherwise messy process...

  17. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...controlling for journal. And so, while our quantitative analysis cannot rule out all other explanations, we do ...ssrn.com/author=1232466.     Neil Thompson is an Assistant Professor of Innovation and Strategy at the MIT...

  18. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...Ecologist     Computer and writing skills Writing a thesis or dissertation demonstrates that you have advanced...what each of these roles involves on a dad-to-day basis. Often, it is hard to know what a job title actually...

  21. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ... prepared me for what a PI without a personal assistant has to deal with. My inbox currently stands at...advisor Wally Gilbert: “Very interdisciplinary, with emphasis on genetic engineering, sequencing, and translation...

  23. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...population is specific for your target antibody and may assist in gating your target population (b). There..., so you can use most of your samples for flow analysis rather than for setting up your initial voltages...

  24. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...The ITRs base pair with each other to allow DNA synthesis of the complementary strand to yield dsDNA. Unlike...ones listed previously). E3/E1B suppresses both apoptosis and the host immune response to the virus to allow...

  25. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ... day of the interview, dress the part, and be consistent with your personal statement, while you make ...success. Every student should use these guidelines consistently across a number of years as you apply for internships...

  26. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...integrate a reporter gene that contains a puromycin resistance gene followed by the target site (the same sequence...:  A donor plasmid that contains a puromycin resistance gene linked to an in-frame H2B-GFP. This visual...

  27. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...clarification, meat tendering, and oligosaccharide synthesis. A major incentive to use cold-active psychrophilic...infinity, and beyond! Keeping in spirit with iGEM’s emphasis on holistic, community-centric science, our team...

  29. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...emphasizing the importance of critical thinking, persistence, teamwork, and communication. And as a program...breathalyzer to pesticide breakdown, production of fire-resistant housing insulation, and cleaning up salt runoff...

  30. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...Less than 20 ng of total gDNA is sufficient for analysis. We have routinely successfully analyzed gDNA .... 6. Fu, Y. et al. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells...

  31. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...Institute were instrumental in helping us establish consistent, high-quality protocols for lentiviral production...at Addgene. Our Board helps keep our focus on assisting scientists and accelerating research and discovery...

  32. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...selected to participate on this panel, but my consistent writing for the Addgene blog (~1-2 blog posts...social media, but how you use social media to consistently engage with others about your topic(s) of interest...

  36. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...can be (part of) figure 1 of your next paper or thesis. This series of posts explains how to critically...Goedhard and Marieke Mastop! Joachim Goedhart is an assistant professor at the Section of Molecular Cytology...

  37. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...journal. An increasing number of funding bodies insist that the research data resulting from their funding...and make up their own minds on whether the data analysis has been of good quality and whether they agree...

  38. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...the inter-molecular annealing or second strand synthesis. In contrast, a scAAV vector with half the size...promote efficient transduction independently of DNA synthesis." Gene therapy 8.16 (2001). PubMed PMID: 11509958...

  39. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...centromeres. These genomic regions are prone to persistent R-loop formation, presumably due to their abundance...considerations when using these reagents. RNA:DNA hybrids Persistent RNA:DNA hybrids that arise during transcription...

  40. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...systematically and logically is as useful for finishing your thesis as it is for helping your team reach an important...thinking, decision making, creativity, or data analysis. Conveniently, these are all skills you are already...
Showing: 281 - 320 of 608 results