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We narrowed to 602 results for: ACE

Showing: 351 - 400 of 602 results

  1. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ... has refined our use of the BLAST web browser interface to be as efficient as possible. If you find yourself...longer alignments to compare with your sequencing trace file. Unlike megablast, the regular blastn program...

  2. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...research uses ancestry groups rather than self-reported race, it highlights the myriad issues researchers need...between biology and ancestral groups or, by extension, race. Many researchers do large-scale CRISPR screens ...

  3. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...individual plasmid orders, because when a PI or lab places an order, they are likely to order several plasmids...next-generation therapeutics. Prime editing, base editing, PACE, and DNA-templated synthesis are four examples of...

  4. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ... journal club? Where will the journal club take place? Preparing for the first meeting There are many ...important pieces before that first meeting takes place to ensure success. One of the most important is ...

  5. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...required for a mini amplification reaction to take place.  When the PCR is complete, a droplet reader extracts...contamination. Optimize your PCR For a good starting place on what PCR parameters to use, see Lock’s seminal...

  6. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...penetration Less resolution due to larger label displacement Require separate incubation of 1° and 2° Must...multiple dyes Greater resolution due to lower label displacement 2° incubation can be skipped by pre-binding ...

  7. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...labeling so that a cell’s projections are easy to trace and not lost in a jumble of fluorescent neurons.... But notice in figure 4b how difficult it is to trace individual cell’s axons, particularly the two green...

  8. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...samples accurately and in a way that can be easily traced. If you’re just starting a lab, or are looking ...barcode can store a lot of information in a small space and is ideal for tracking large collections. Many...

  10. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...across from the abasic site before filling the empty space. Over several rounds of rational mutagenesis, the...editors Excises G, T, or C, respectively, to be replaced with another base. References Chen, L., Hong...

  11. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...struggling to understand how the researchers decided to place their gates on the plots! Flow data is messy, so...., Pons, J. A., & Muro, M. (2018). In vitro intracellular IFNγ, IL-17 and IL-10 producing T cells correlates...

  12. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...commercial antibodies can be tethered on the viral surface, expanding the range of targetable cell types. ...et al. (2023). Time-tagged ticker tapes for intracellular recordings. Nature Biotechnology, 41(5), 631...

  13. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...preparations are purified by iodixanol gradient ultracentrifugation and concentrated to purity and titers adequate...maintained for ~20 passages before being discarded and replaced with a new vial of early passage cells. Approximately...

  14. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...without disrupting plasmid representation. A good place to start is with a protocol for a library of similar...Moderated by the Feng Zhang lab, this is a useful place to ask experimental questions.  Practical Considerations...

  15. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ... and vary in shape and structure. Their porous surface provides a large area for binding, but antibodies... than agarose beads. Proteins conjugate on the surface of magnetic beads, and are therefore completely...

  16. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...are 27 incompatibility (Inc) groups in the Enterobacteriaceae family alone, and this is expanding as we...expelled antibiotic resistant plasmids from Enterobacteriaceae family members within the mouse gut (Kamruzzaman...

  18. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...through the stomata into the abaxial site (lower surface) of the leaf. This is a simple and easy technique...Mortensen et al., 2019): Leaves or whole plants are placed in an Agrobacterium solution and a vacuum is applied...

  20. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ... or the statistical variation is calculated and placed in the legend. Widely reported difficulties in ...on the fly”. This becomes intuitive with touch interfaces where the viewer is more likely to want to manipulate...

  21. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...structural complexity inside the cell or on its surface. Bear in mind, the voltage setting of the laser...through conjugated antibodies targeting the cell surface markers CD11 (anti-mouse CD11b-BV510), which is...

  22. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...expressed on T cells to make CAR T cells. The extracellular scFv portion of the receptors recognizes an ...an antigen of interest while the intracellular T cell receptor portion of the CAR facilitates signal transduction...

  23. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ..., their makeup (in terms of nationality, gender, race, etc), or their career outcomes. Some of the more...their mentorship with the same critical eye they place on others within their labs - as a sort of over-...

  25. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...but is activated by GAL4 binding to UAS. If you place the GAL4 gene downstream of a tissue- or developmental...inhibiting the binding of GAL4 to UAS. One can therefore place GAL4 and GAL80 under different promoters and create...

  26. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...and the ATP dependent 9°NTM DNA Ligase are not replacements for T4 DNA Ligase in applications such as routine...PMCID: PMC306403. 11. Wu, Dan Y., and R. Bruce Wallace. "Specificity of the nick-closing activity of bacteriophage...

  27. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...function. After analysis, CRISPR classifications were placed into a fairly simple hierarchy: class to type to.... However, knowing the basic diversity and the surface-level function of each type could lead you to new...

  28. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ... presence or absence of certain markers on the surface or the inside of your cells. While proteins themselves...plasma membrane and can be detected through a cell surface label. The viral vector also encodes for eGFP, ...

  30. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...multiplex your reference genes to save on plate space. For many cell lines and tissue types, appropriate...your reference gene set can save significant plate space and may be worth the initial effort of setting it...

  31. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...stability Limited protein size range Tris-acetate 40–500 kDa Tris-tricine 150 V, 1–3 hours ... simply wet your membrane with 20% methanol and place it on a light box after drying. Nitrocellulose, ...

  32. Addgene’s First AAV Data Hub Challenge

    Type
    Blog Post
    Published
    Sept. 20, 2022, 1:15 p.m.
    ...Addgene’s first AAV Data Hub Challenge, now taking place Dec. 6, 2022! Share your AAV data, connect with ...

  34. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ...vector terms. And, the name Kendall has a special place in our hearts: our first two offices were located...

  36. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ...Addgene gave to me: the PEDAR strategy to delete and replace long genomic sequences. On the ninth day of CRISPR...

  38. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ...Cytometry, Designing Your First Flow Panel, and Beyond Surface Labeling Ashlyn Lemmen, who shared her flow cytometry...

  40. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ...It's just unfortunate that we had no choice but to place it next to our lab trash (which coincidentally matches...

  41. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    .... Addgenies swapping out printer cartridges can place the spent cartridge in the large cardboard box in...

  43. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...!   CRISPR Cheat Sheet Clustered Regularly Interspaced Short Palindromic Repeats: Sequences of DNA found...

  45. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...this year’s Addgene costume contest, like us on Facebook and checkout our FB page on Friday for more photo...

  46. Newly Updated AAV Data Hub!

    Type
    Blog Post
    Published
    Feb. 7, 2023, 2:15 p.m.
    ...revamped, and re-launched Addgene AAV Data Hub, the place for scientists to share their AAV validation data...

  47. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    Published
    Jan. 25, 2021, 5:54 p.m.
    ...topics in the dropdown menu. These pages are a great place to start if you’re new to the topic. Or, try the...

  49. Addgene Stands with the Scientific Community

    Type
    Blog Post
    Published
    April 23, 2025, 1:30 p.m.
    ...demonstrated remarkable resilience and creativity in the face of funding challenges. By strengthening our commitment...

  50. The Addgene Antibody Data Hub

    Type
    Blog Post
    Published
    Sept. 12, 2024, 1:15 p.m.
    ... process, so you can work through it at your own pace. To submit, simply click "Contribute Data."    ...
Showing: 351 - 400 of 602 results