.... Find Plasmids from the publication at Addgene 2. Green, Alexander A., et al. "Toehold switches: de-novo-designed...the project were able to move forward quickly in parallel. More broadly, we drew on the combined creativity...

...of 3,5-difluoro-4-hydroxybenzylidene-imidazolinone-2-oxime (DFHO), a fluorophore modified from DsRed. DFHO...intracellular viral DNA dynamics in neuronal cells (Nilaratanakul et al., 2017) and levels of the second messenger... a “Top” and “Bottom” portion, each fused to a separate piece of RNA. Fluorescence is observed when these...23488798. PubMed Central PMCID: PMC3775879. Nilaratanakul, Voraphoj, Debra A. Hauer, and Diane E. Griffin...Griffin. "Development and characterization of sindbis virus with encoded fluorescent rna aptamer spinach2...et al. "Ribonucleoprotein purification and characterization using RNA Mango." RNA23.10 (2017): 1592-1599...

...start selecting and testing systemic capsids! Figure 2 contains a decision tree for selecting a capsid, while... highlighted below the table.     Figure 2: This decision tree can help guide your choice on ...transfer to the adult brain. Nature Biotechnology, 34(2), 204–209. https://doi.org/10.1038/nbt.3440 Goertsen...Thomson, M., & Gradinaru, V. (2021). Deep Parallel Characterization of AAV Tropism and AAV-Mediated Transcriptional..., with significant contributions from Cynthia Arokiaraj. Addgene would also like to thank Viviana Gradinaru.../doi.org/10.3389/fimmu.2021.730825 Campos LJ, Arokiaraj CM, Chuapoco MR, Chen X, Goeden N, Gradinaru V...Adams, C. D., Yang, D., Wang, T., Wolfe, D. A., Arokiaraj, C. M., Ngo, V., Campos, L. J., Griffiths, J. ...

... 2008 Dec; 18(6): 617–623. Pubmed PMID 19349161.  2. Deshpande A, Bergami M, Ghanem A, Conzelmann KK, ...Tsiang H, Ceccaldi PE. Spread and pathogenic characteristics of a G-deficient rabies virus recombinant: ...

...Reports 9: https://doi.org/10.1038/s41598-019-41088-2 Magnus C, Lee P, Bonaventura J, Zemla R, Gomez J, ...to one receptor, so it is important to fully characterize the compound, or compounds, you choose to ensure...

... 24901218. PubMed Central PMCID: PMC4047077. 2. Pagliara, Patrizia, et al. "Ostreopsis cf. ovata induces...

... the Cas machinery where to cut.      Fig. 2: Heatmap indicating loci where SNPs reside on sgRNA...from work being done today, is a nearly entirely separate effort from acknowledging its existence. Studying...

... replication competent virus [usually HIV-1]; and 2) the potential for oncogenesis through insertional... are technically gamma-retroviruses - another, separate member of the retroviral family. While both lentiviruses...

... more from the authors in this blog post. Figure 2: iGluSnFR3 displays enhanced brightness, expression...adapted from Farahani et al. 2022, under CC-BY license.   Find pYtags plasmids here!   Farahani PE, et al...for monitoring specific RTK activation in vivo (Farahani et al. 2022). The authors take advantage of the...better response kinetics and photochemical characteristics than previous NIR calcium reporters iGECI and...   Matlashov ME, et al. Design and Initial Characterization of a Small Near-Infrared Fluorescent Calcium...for lactate developed using a high-throughput multiparameter screen (Koveal et al. 2022). Lactate is a key... here!   Koveal D, et al. A high-throughput multiparameter screen for accelerated development and optimization...

..., whereas the mVenus-APT1 fusion does not (figure 2). In the mVenus-APT1, a lipidation motif, which is...targeted to the endoplasmid reticulum based on the appearance of OSER whorls. This assay is quantitative and...also collected data on fluorescent protein characteristics such as the brightness, photostability, maturation...Cranfill et al, 2016; Lambert, 2019). These characteristics are important, but have less priority than ...microscopy (University of Amsterdam). He develops, characterizes, and uses genetically encoded fluorescent probes...PMC4383620. Botman, Dennis, et al. "In vivo characterisation of fluorescent proteins in budding yeast."...brightness, photostability and dynamic range: characterization of dedicated sensors for FLIM, for ratiometry...

...Nature 339 (1989). 487–488. PubMed PMID: 2725683. 2. Miller, C.J., Kassem H.S., Pepper S.D., Hey Y., Ward... storage buffers used, and other experimental parameters. For example, while freeze thaw is generally ...25073060. 8. Finkelshtein, D., Werman, A., Novick, D., Barak, S., Rubinstein, M. “LDL receptor and its family...

... PMID: 9537413. PubMed Central PMCID: PMC3010411. 2. Kohli, Manu, et al. "Facile methods for generating...and virtually never exceeds 40%. While this is comparable or better than the efficiency of most current...ribonucleoproteins (RNPs) that can be supplied separately from the viral vector. This delivery method frees...

...vectors with EYFP split at 175 Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components...

...rules are: (1) don’t use the same overhang twice; (2) avoid palindromes; (3) no overhangs with the same...highest-fidelity overhang set it can find for those parameters. For even more specificity, you can exclude specific...

...systems utilize with type I-F Cascades. Figure 2: Cascade complex from CRISPR type I-E. Created with...been challenging. It can be difficult to ensure comparable expression of all components of Cascade for maximum...

...that view (Callahan et al., 2022).     Fig. 2: Students in an MDH CURE replaced select residues ...Part I – guided inquiry – purification and characterization of a fusion protein: Histidine tag, malate...

...interesting things to do, feel free to crowdsource! 2. Free food generally gets people to show up If you... company culture here at Addgene has remained paramount. Candidates who interview at Addgene frequently...

...experience and to build upon your existing skills.  2. Find your niche Identify and develop an area of expertise...seekers to use online applications with significant character limits for the written portion of the grant application...

...externally fertilized, transparent progeny (Figure 2). These features give it a leg up (or a fin up?) over...time and space, and was also used to precisely characterize developmental defects in the one-eyed pinhead...

...based antiviral method to degrade RNA from SARS-CoV-2 sequences and live influenza A virus. It was demonstrated...stuck in inclusion bodies. Takayuki Iwaki, Kimiko Hara, and Kazuo Umemura have described a simple method...

...rate of 10-55% - the PASTE system.     Fig. 2: Schematic of programmable gene insertion with PASTE...and Discovery As part of their quest to find, characterize, and develop new CRISPR systems and technologies...

...two visible blue or magenta chromoproteins (Figure 2) (Liljeruhm et al., 2018). This system is a nice confirmation...tested their expression under both rhamnose- and arabinose-inducible promoters. Tight control of toxin expression...escape). The rhamnose induction system is well-characterized and quite tight (Giacalone et al., 2006). The...though Khetrapal et al. also demonstrated that the arabinose promoter worked too. Rhamnose induction relies...References Espah Borujeni, Amin, Anirudh S. Channarasappa, and Howard M. Salis. "Translation rate is controlled...

... iRFP713, % Oligomeric state Photo-stabilityc, t1/2, s pKa Brightness in HeLa cells vs. iRFP713, % d ...and multicolor NIR imaging.  Small miRFPnano A separate class of NIR FPs, named miRFPnanos, were derived...developed based on mIFP6. Learn more about the characteristics, performance, and applications of near-infrared... found to be a dimer3,15. ND-not determined in parallel assays with other FPs.   We hope that this post...

...can still record the signal separately for each target.   Figure 2: Examples of simultaneous multiplex...or formalin-fixed, paraffin-embedded tissue sections, methanol- or paraformaldehyde-fixed cell samples...samples, or other preparations. Sequential IF For a sequential IF experiment (Figure 1A), you’ll block, add...different color fluorophore that you can image separately on your microscope. Abcam's sample protocol is...different antibodies, blocking, washing, and sample preparation steps, in order to ensure your antibodies are...Ilangovan, V., Lo, H., Olson, M., Mohamed, S. Y., Sarabipour, S., Varma, A., Walavalkar, K., Wissink, E. M...

...variety of environmental conditions, and double every 2 hours. Yeast are also the first eukaryotic genome ...as Parkinson’s disease (PD). PD is primarily characterized by protein misfolding that leads to a build-...23594743. PubMed Central PMCID: PMC3703927. Jennings, Barbara H. "Drosophila–a versatile model in biology & medicine...

...become a valuable asset yourself.     Fig 2: A diagram of the Thomas-Kilmann Conflict Model, courtesy...favorite books, Michael Ende’s Momo, the title character endears herself to the nearby village (and to ...or how completely different teams are tackling comparable conflicts. Is this level of domain knowledge ...

...There are approximately 2-4 million proteins per cubic micron in bacteria, yeast, and mammalian cells...proteins can then be purified using streptavidin and separated via flow cytometry.  Find plasmids for EXCELL ...

... Watch Joanne give her "Not" Networking 101 Talk 2. Tailor every talk to the occasion Don’t just plan...come up with clear and concise answers. This preparation will help you avoid giving only yes or no answers...

...PubMed PMID: 3459152. PubMed Central PMCID: PMC323600. 2. Chester, Nicholas, and Daniel R. Marshak. "Dimethyl...small difference in size, the fragments should be separated on a high percentage agarose gel (~3%). Note that...

... from damage (Abe & Lim, 2024).    Figure 2: A) When proteins (blue) interact with the air-water...RvLEAMshort (1:6 molar ratio) with 10 minutes of glutaraldehyde crosslinking: representative micrograph (C)...allowed the authors to determine structures at comparable or higher resolution than using more complex ...buffer additive MgCl2 and the crosslinking agent glutaraldehyde. Adding RvLEAMshort to the sample before plunge...

...biology 14.6 (2004): 757-764. PubMed PMID: 15582400. 2. Tomkinson, Alan E., et al. "DNA ligases: structure...the ligation junction were represented. Sixteen separate pools were prepared, each with a different complement...applications such as routine cloning or DNA library preparation. However, when a method relies on accurate ligation... 19329793. PubMed Central PMCID: PMC2719376. 5. Barany, Francis. "Genetic disease detection and DNA amplification...PMID: 1986365. PubMed Central PMCID: PMC50775. 6. Barany, Francis, and David H. Gelfand. "Cloning, overexpression...PMCID: PMC208194. 8. Luo, Jianying, and Francis Barany. "Identification of essential residues in Thermus...76.2 (1989): 245-254. PubMed PMID: 2753355. 12. Barany, Francis. "The ligase chain reaction in a PCR world...

...experience in cancer research After graduating with a 2:1 BSc in Molecular Biology (roughly a B average in...really passionate about during my degree. I loved parasitology. However, when I enquired about roles in this...end of my three months, I fully understood why parasitology roles in the field require experience. Working...beginning of 2010, I explored options for going into parasitology but another major factor held me back; I do ...

...Nutr, 129 (2009a): 477–484. PubMed PMID: 10064313. 2. Chapman-Smith, A., & Cronan, J. E. “The enzymatic...Forster, A. C., Skingle, D. C., & Symons, R. H. “Preparation and uses of photobiotin.” Methods in Enzymology...

...effective recovery of knock-ins, but did result in 1-2 kilobases of additional sequence being introduced....brilliantly and irreverently illustrated in the parable of the Geneticist vs the Biochemist, we geneticists...selection allowed me to optimize a range of editing parameters: oligo repair templates with homology arms of... template design The Meyer lab meticulously characterized several aspects of editing. First, they devised...doi.org/10.17912/DDVH-BG64 Tzur YB, Friedland AE, Nadarajan S, Church GM, Calarco JA, Colaiácovo MP (2013)...

...Single-chain variable fragment (ScFv) Figure 2: Comparison between the IgG antibody and scFv. ...the surface of extracellular pathogens such as parasites or microbes, or to proteins expressed on the surface...containing a mixture of proteins. The proteins are separated based on molecular weight by SDS-PAGE, and are...

...ask new and interesting questions in your own lab. 2. Seek multiple mentors during your postdoc At the ...corresponding author publications (published, not in preparation) A history of successful funding (fellowship ...