We narrowed to 444 results for: Ank
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TypeBlog PostPublishedFeb. 4, 2025, 2:15 p.m....have gotten larger, easier, and more comprehensive thanks to the consistent development and improvement of...
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AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog PostPublishedSept. 12, 2017, 1:44 p.m....distributing the vectors? Viral genome sequencing Thanks to the establishment of in-house sequencing capabilities... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog PostPublishedSept. 7, 2017, 12:17 p.m....gel solution discussed above for picking. Many thanks to our guest blogger Iris Lindberg, Professor at... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog PostPublishedJuly 13, 2017, 2:30 p.m....fundamentals about how many of these resources work. Many thanks to our guest blogger, Jon Chow! Jon Chow is finishing... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog PostPublishedJan. 10, 2017, 3:37 p.m.... or repression at the respective sites. Many thanks to our guest bloggers Alissa Lance-Byrne and Alex... -
3D Printing Meets CRISPR Cas9
TypeBlog PostPublishedOct. 17, 2017, 12:55 p.m....make the molecular world real for students. Many thanks to our guest blogger Tim Herman! TIm Herman is ... -
Plasmids 101: Degron Tags
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m....structures, interacting partners, and localization. Thankfully, this is a hot topic with many resources! In ... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog PostPublishedFeb. 4, 2020, 2:15 p.m.... from fluorescent jellyfish, bioluminescent phytoplankton, and colorful corals, to name a few. What’s ... -
Academic vs. Industry Postdocs
TypeBlog PostPublishedNov. 13, 2018, 1:22 p.m.... you with the next step in your career. Many thanks to our guest blogger, Laura Desrochers! Laura ... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog PostPublishedOct. 30, 2018, 1:04 p.m....another, also known as transdifferentiation. Many thanks to our guest blogger, Kusumika (Kushi) Mukherjee... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog PostPublishedOct. 23, 2018, 11:49 a.m....Please share them in the comments below! Special thanks for Dr. Dan Stone and Harshana de Silva Feelixge... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m....Other Argonaute Plasmids from the Doudna Lab Many thanks to our Guest Blogger Pooran Dewari! Pooran Dewari... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog PostPublishedApril 19, 2016, 2:30 p.m....your chances of finding artifactual results. Many thanks to our guest blogger Erik L. Snapp! Erik Lee Snapp... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog PostPublishedSept. 24, 2015, 2:30 p.m....component delivery with validated tools. Special thanks to Joel McDade for creating the Expression Systems... -
Plasmids 101: Multicistronic Vectors
TypeBlog PostPublishedSept. 9, 2014, 8:20 p.m....with a plasmid that has multiple cloning sites flanking the multicistronic element(s), or you could replace... -
Tips for Using BLAST to Verify Plasmids
TypeBlog PostPublishedMay 29, 2014, 1:29 p.m....collection (nr/nt)” as it contains a composite of GenBank, EMBL, DDBJ, and PDB sequences and may be the most... -
Which Fluorescent Protein Should I Use?
TypeBlog PostPublishedMay 20, 2014, 2:06 p.m....your work, head over to greenfluorescentblog. Thank You to Our Guest Blogger! Gal Haimovich, PhD, is... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m....dynamics of PPIs are the focus of study. Many thanks to our guest blogger Patrick Miller-Rhodes from... -
CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m....prefer targets with a given single base protospacer flanking site (PFS) sequence, but orthologs like LwaCas13a... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog PostPublishedJuly 16, 2020, 1:15 p.m.... results for China with more uncertainty. Many thanks to our guest bloggers Max W. Shen from MIT, Alvin... -
Summer SciComm Series: Modes of Communication
TypeBlog PostPublishedJuly 1, 2022, 1:15 p.m....to choose Where is your audience? It’s, quite frankly, much easier to go where your audience is than ... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog PostPublishedFeb. 27, 2018, 2:04 p.m....Check out the NIH’s Developmental Studies Hybridoma Bank (DSHB). 1 ml of antibody-containing hybridoma supernatant... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog PostPublishedOct. 10, 2023, 1 p.m....in bacteria that express the Tn5 element, but thankfully, most competent cells do not express this factor... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog PostPublishedSept. 28, 2017, 2:01 p.m.... In this special CREATE cap plasmid, loxP sites flank the downstream polyA sequence. When viruses produced... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog PostPublishedJan. 11, 2018, 3:29 p.m....from a client you helped. Maybe you awkwardly say thanks and move on with your day, feeling good but focused... -
Simplify Cloning with in vivo Assembly
TypeBlog PostPublishedOct. 18, 2018, 12:37 p.m....homologous sequences to the insert by PCR. Many thanks to our guest bloggers Jake Watson and Javier García-Nafría... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog PostPublishedAug. 9, 2016, 2:30 p.m....-L 9 -N 1 -U GRNA_23MERS -S GRNA_HITS.sam Many thanks to our guest blogger, Aneesh Karve! Aneesh Karve... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog PostPublishedSept. 3, 2015, 2:30 p.m....employees spent this remaining time by elaborately pranking each other, dressing in costume, and holding competitions... -
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog PostPublishedNov. 19, 2020, 2:15 p.m....patient-derived iPSCs, MECP2 animal models, tissue banks, Rett databases, and other helpful tools. References... -
To Codon Optimize or Not: That is the Question
TypeBlog PostPublishedNov. 12, 2020, 2:15 p.m....Ikemura lab created the Codon Usage Tabulated from GenBank database (CUTG), that provides an electronic dataset... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m.....1231143 Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F (2017) RNA editing... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog PostPublishedApril 13, 2021, 1:15 p.m....systems a try, we’d love to hear your feedback! Thank you to our guest bloggers! Qi Li obtained her Ph.D... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog PostPublishedSept. 17, 2019, 12:40 p.m....rodents. In this test, an animal is placed into a tank of water with no support from below or anything ... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog PostPublishedAug. 13, 2019, 12:58 p.m....likely to continue with help from zebrafish. Many thanks to our guest blogger Katherine Rogers from the ... -
Save Time with Transient Plant Leaf Transformations
TypeBlog PostPublishedJuly 25, 2019, 12:03 p.m....and experiences in the comments below. Many thanks to our guest blogger Samuel Mortensen from Northeastern... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog PostPublishedJuly 2, 2019, 12:51 p.m....experimental goals. If not, give CRISPRa a go! Many thanks to our guest blogger Patrick Miller-Rhodes from... -
Lab to Office Culture Shock
TypeBlog PostPublishedFeb. 5, 2019, 1:04 p.m....you start looking for them. Acknowledgements Big thank you to Jessica Welch, Mike Lemieux (for his advice... -
CRISPR-mediated Plant Base Editors
TypeBlog PostPublishedJan. 3, 2019, 1:35 p.m....may also results in RE site destruction. Many thanks to our guest blogger Kutubuddin Molla from the ... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog PostPublishedJune 20, 2024, 1:15 p.m.... protein overexpression might skew the results. Thanks, stringent regulation! This post was originally... -
Neuronal labeling with Spaghetti Monster
TypeBlog PostPublishedAug. 14, 2018, 12:42 p.m....serotypes to target the neurons they’re studying. Thanks to this work it is now much easier to express molecules... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog PostPublishedMay 8, 2018, 1 p.m....Synthego blog for more great CRISPR content. Many thanks to our guest blogger, Jessica Roginsky. Jessica... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog PostPublishedSept. 21, 2017, 2:07 p.m....when 2 mismatches are present. The protospacer flanking sequence (PFS) for LshCas13a, which is analogous... -
How to Write a Scientific Review Article
TypeBlog PostPublishedFeb. 16, 2017, 3:30 p.m....conserved domains. This information was available in GenBank, but it was useful to present it all in one place... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog PostPublishedOct. 13, 2016, 2:30 p.m....stepping-stone to many other opportunities. Many thanks for our guest blogger, Gairik Sachdeva! Gairik ... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog PostPublishedDec. 14, 2017, 2:08 p.m.... pCXWB-EBNA1 37624 transient EBNA-1 Many thanks to our guest blogger, Kusumika (Kushi) Mukherjee... -
Transferable Skills Guide: Career Planning Resources
TypeBlog PostPublishedNov. 14, 2017, 2 p.m....return, make sure to follow up with a professional “thank you” via email. You’ve now made a connection, and... -
Career Insights: Technical Support Specialist
TypeBlog PostPublishedNov. 9, 2017, 2 p.m....jumping off point for other roles in industry. Many thanks to Eric Perkins and Stephanie Hays for images used... -
CRISPR Activation: A Practical Guide
TypeBlog PostPublishedAug. 18, 2016, 2:30 p.m....well. Best of luck on your experiments! Many thanks to our guest bloggers Marcelle Tuttle and Alex ... -
Tips for CRISPR Gene Editing in Mice
TypeBlog PostPublishedJune 28, 2016, 10:59 a.m....mammalian model organisms in the near future. Many thanks to our guest blogger Samantha Young. Samantha Young... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog PostPublishedOct. 27, 2015, 3 p.m.... moxFPs are now available through Addgene Many thanks to our guest bloggers Erik L. Snapp and Lindsey...