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We narrowed to 609 results for: CaS;

Showing: 351 - 400 of 609 results

  1. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ... is not suitable for use in apoptotic cells as Caspase3/7 both cleave after the DVPD sequence. Check out...protein functionality (Arribere, et al). In rare cases, tags can also affect protein localization and solubility...

  2. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...of a hairpin structure within your primer. In our case, we will add TAAGCA, resulting in a final Forward...to the sequence to be amplified (the ORF in this case), not the Tm of the entire primer. If you are amplifying...

  3. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...computer modeling and predictive algorithms to forecast the drug epitopes likely to be presented by T ...between 2D assays and in vivo animal models. In these cases, they are designed to mimic the human lymphatic ...

  4. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...really matters. Well, it turns out this isn’t the case for using AAV. When it comes to infecting cells,... delivering receptor constructs with AAV. In the case of channelrhodopsins, fewer channel proteins are...

  5. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...we all shared a passion for DIY Biology and, on occasion, would meet in the same space where BosLab exists...journal club discussion on Kevin Esvelt’s CRISPR/Cas9 gene drive paper was very popular. People kept asking...

  6. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ... common event. What are exosome AAVs? In 2012, Casey Maguire and colleagues from Massachusetts General...28082074. PubMed Central PMCID: PMC5368844.  Maguire, Casey A., et al. "Microvesicle-associated AAV vector as...

  8. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ... strategic knowledge to solve a clinical disease case. As a teacher, you explain the game rules and then...face each other on a single chosen clinical disease case. Then, each team received a whole set of cards (...

  9. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ... start of the CRISPR craze, but he says, “In the case of CRISPR, the hype was completely justified… it...the audio engineer and producer for the Addgene Podcast.       Resources at Addgene Find CRISPR resources...

  10. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...of having all the pages in a Curricula Vitae to showcase your publications, academic performances, and ...related to the job you are applying for, in which case talk about your research, as an understanding of...

  11. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...should be interesting; however, this should (in most cases) not be the driving factor. The following questions...head for a career in pharma or biotech, this is one case where the topic of your research can make your transition...

  12. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...up positive traits (i.e. the plasmid contains a cassette that will make cells resistant to a toxin), whereas... a negative trait (i.e. the plasmid contains a cassette that will make cells sensitive to a toxin). In...

  13. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...validate your antibody on crosslinked proteins. In the case of histone modifying proteins, which bind quite ...abundance transcription factor or cofactor. In such cases, the gentler enzymatic digestion can preserve more...

  14. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...photons or odorants, to elicit downstream signaling cascades.  So let’s break down the name "G-protein coupled...then induce or inhibit intracellular signaling cascades as a response to the extracellular stimuli. Ligand...

  16. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...following exposure to a brief heat shock. In the case of Hsp70, the heat shock releases heat shock factor...depositors have developed heat shock-inducible Cre and Cas9 for easy genome engineering in species like C. elegans...

  17. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...during in vivo research and the CARE guidelines for case reports are two sets of guidelines frequently used...manuscript is accepted for publication and is, in most cases, blind (meaning you don’t know who the reviewers...

  18. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...primary-conjugated) or indirect (secondary) methods. In those cases, remember that a secondary should be used when signal...you still have a number of options. If this is the case, you can narrow them down in a variety of ways. ...

  20. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...given threshold within the cell, as is often the case upon viral infection. Endogenously produced dsRNA...the Addgene blog CRISPR 101: RNA Editing with Cas13  RNA Interference in Plant Biology  ...

  21. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...intensity remains. The first issue is that, in several cases, the fluorescence intensity decay of a fluorophore... the excitation power (Grünwald et al, 2008) occasionally and certainly after a set-up has been changed...

  22. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...oligonucleotides specifying a gRNA target sequence into a Cas9-containing plasmid such as pX330. This cloning strategy...are using the Golden Gate method to create CRISPR/Cas9 constructs, assemble standard plasmids parts in ...

  24. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...large (1-10) and small (11) fragments. In several cases an FP(1-10) fragment may be compatible with multiple...Split-horseradish peroxidase (Alice Ting Lab 2016)  Split-Cas9 for inducible genome editing (Feng Zhang Lab 2015...

  25. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...conjugated substrate is added to the wells. In the case where the detection antibody is biotin-conjugated... protein within a mixed sample. However, in this case your protein of interest is pre-incubated with a...

  27. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...the heading "Addgene Partial NGS Result." In some cases, there will be more information about our sequencing..., Sanjana NE, Zhang F (2017) Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...

  28. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ... that expresses well, then probably not. In this case, take a look at the secondary antibodies that your...to probe multiple targets in parallel. In these cases, it is critical that users choose antibodies that...

  29. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ... process, following sequencing and assembly. In cases where the QC team suspects an assembly issue, they...and be made available for distribution. In these cases, the QC team leaves a note on the plasmid page to...

  30. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ... Let’s get started! Before you begin In the best case, you know ahead of time that you want to probe your... to lose by trying to strip and reprobe. In this case, we recommend using mild stripping solution (see...

  31. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ... and serve as a type of positive control. In the case of an unfavorable donor-acceptor orientation limiting...molecules, detect cellular processes and signaling cascades, quantify mechanical tension (a molecular “spring...

  33. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...breaches such as the Tuskegee syphilis experiment, the case of Henrietta Lacks, the Havasupai incident, and ...schools, community events, churches or mosques. On occasion, we also get interviewed for print media, radio...

  34. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...the paternal for Prader-Willi syndrome). In both cases, the other copy of the chromosome is normally imprinted... exact genes, being improperly silenced in both cases. In both diseases, genes from both copies of chromosome...

  35. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...potentially costly set-ups. However, there are many cases where the specific viral particles you need are ... viruses are too high for your budget. In those cases, you’ll need to produce your viruses in-house. ...

  36. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ... providing feedback is needed for that. In many cases reports are not aware of issues until you bring ...what to do. Unfortunately this is not always the case or you may not feel comfortable reaching out to ...

  37. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...your intellectual freedom and integrity. (Not the case, as I’ve discovered.) As someone who has now seen...academia, as many of us know, this is not always the case. There are “difficult personalities” in every work...

  38. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...hits come from these known impurities, as was the case for clean sample AAV-44362. In addition to these...containing the suspected contaminating sequence. In most cases, the source of the contamination can be identified...

  40. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...can be faster and easier to work with. In either case, it can be useful to run a restriction digest of...   For CRISPR libraries, Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...

  41. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...fluorophore is present in the sample (APC in the case of Figure 1). In Figure 1A, the population needs...shifted, consider your perpendicular lines. In this case, the population on the left is a horizontal line...

  42. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...that the emission wavelength can change. In a few cases, the initial state of the protein is non-fluorescent... yield of the protein, divided by 1000. In many cases the brightness is compared to that of EGFP which...

  43. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...responsible for each figure. Others still are more casual with a single presenter summarizing the paper’s...could be solicited via multiple avenues including casual request via email, in person, or instant messaging...

  44. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...the sample to allow for even expansion.  In some cases, labels are added after this stage.  Step 4: Expand...alleviated by maximizing label density. In some cases, nanobodies have been found to help improve label...

  45. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...activation sequence (UAS) of its target gene, in this case a reporter gene (e.g. luciferase or GFP), regardless... system has been devised (8). References 1. Casadaban M.J., Martinez-Arias A., Shapira S.K., Chou J....

  46. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ... adults alike enjoyed some friendly -or in some cases, serious-  competition with our corn toss boards...word of mouth - be sure to bring up your event in casual conversation to get people excited!    Laura ...

  47. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...copy number is high (del Solar et al., 1998).  In cases of compatible plasmids, different negative regulation...replication or partitioning machinery (Novick, 1987). In cases where one plasmid outcompetes the other, usually...

  48. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...the first time. They can then plan accordingly in case you have questions or need a little assistance at...questions later – maybe even the next day. In the latter case, you can say “I usually need time to gather my thoughts...

  49. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...Addgene relied on Sanger sequencing for QC. We occasionally still use Sanger sequencing to confirm an insert...are highly repetitive, such as those containing cassettes of the same gene multiple times.  Bioinformatics...

  50. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...other tools in conjunction with the ELN, in some cases the integrations provide a direct boost to reproducibility...restrictions based on permissions, however, in some cases it would be possible to grant those wanting to understand...
Showing: 351 - 400 of 609 results