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  3. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...protein purification is provided by GE Healthcare Life Sciences. Addgene pages and blog posts of interest...

  9. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...that this is just a review article and not your life’s work: so remember that done is better than perfect...

  16. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ... proteins in heterologous hosts. If you simply amplify a gene of interest from the human genome, for instance...

  18. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...attention! They isolated DNA from the sample, amplified the CRISPR locus from the bacterial genome, and...

  19. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ... (2014) A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging....

  23. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ... a broken relationship. Pardo B, et al. Cell Mol Life Sci (2009). PubMed. Regulation of DNA double-strand...

  24. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...   How to perform a TIDE First, use PCR to amplify the target region of interest. Then Sanger sequence...

  30. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...talks We are not talking about lab meeting here. Lifeless presentation of experimental results in the order...

  31. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...interest by recording DNA abundance throughout the amplification process, rather than just at the end as in standard...

  33. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...vectors. Used by Addgene for pooled library amplification. F' mcrA Δ(mcrBC-hsdRMS-mrr) recA1 endA1 gyrA96...

  35. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ... pathways. Additionally, as GPCR signaling is amplified in successive steps along a pathway, there can...

  42. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...address these concerns as CRISPR use grows across the life sciences and to establish standardized validation...

  45. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...antibodies can bind to a primary antibody thus amplifying the signal.       Intracellular protein targets...

  46. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...using BsaI, and promoter-gRNA fragments are PCR amplified for cloning into one of three Zeamays codon-optimized...
Showing: 351 - 398 of 398 results