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We narrowed to 612 results for: SCR

Showing: 351 - 400 of 612 results

  1. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...used to quantitate RNA levels using reverse transcription-qPCR (RT-qPCR). The first step of this process...requires RNA to be converted to cDNA using reverse transcription and the cDNA is subsequently quantified by qPCR...

  2. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...processes. To create the antibody toolbox, they screened antibody candidates using a synthetic human scFV...cross-reactivity, antibody candidates underwent affinity screening to select for slow off-rate binders. The best ...

  3. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...while seated or with your poster on your computer screen than it is to pretend you’re standing at the session...poster board well ahead of time ensures you aren’t scrambling to tack up your poster seconds before the session...

  4. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...component as well as a small molecule which we will describe in more detail below. Types of Voltage Indicators...membrane, a concern with several of the previously described systems. Several adaptations of this method have...

  5. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right: T1 tomato plants that inherited an edited...for its improved kinetics and responsivity in screening ASAP3 variants. B) Electrical and optical recordings...

  6. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...sense, models can be both useful, and dangerous. Subscribe to CRISPR Blog Posts How do educators and students...reaction to this model on his blog. Or, for a description of how effective this model was in explaining...

  7. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...After this initial application, Stelzer’s group described the single-plane or selective-plane illumination...optical sectioning of a specimen is based on discriminating the out-of-focus reflected light by using a...

  9. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...well in plants and give similar stability to transcripts as the ones that people use all the time. Now... Marillonnet. ACS Synth. Biol., Just Accepted Manuscript, DOI: 10.1021/sb4001504. Journal. A modular cloning...

  10. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...standards (clear and detailed methods and image descriptions, a minimum n = 2, and well labeled high- and...’ results is available not through published manuscripts, but through word of mouth or old lab notebooks...

  11. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...plasmid purification and DNA gel extraction method described in Kim and Morrison is nearly identical to that...Additionally, while commercial plasmid prep kits come in discreet sizes (mini, midi, maxi, giga) which requires ...

  13. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...HCab antibodies from its serum, and meticulously screening, optimizing, and characterizing several anti-mouse...review. If you just want to use the nanobodies described in Pleiner et al, the key steps for generating...

  14. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...expression) and a polyA signal (to terminate transcription) and the four constructs were then combined ...their 6-mer sequences, are rarely found in the transcriptomes of these species and can often be used to clone...

  15. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...two pieces: a single protein, computationally described as a fusion of four Cas7 proteins and a putative... of landing sites via Cas9-directed reverse transcriptases, followed bylanding site recognition and integration...

  16. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...VSV-G is also toxic at high concentrations as described above and is inactivated by the complement system...proteins that can be used for specific purposes as described previously (Table 1, Gutierrez-Guerrero, et al...

  17. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...CRISPR-associated transposases” or MUCICAT. Here, we’ll describe each of these CRISPR systems. The original pCas...chromosomal integration of cargo without using screening markers. Capability to construct a strain library...

  18. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...synthesis companies, such as IDT, Genewiz and GenScript, offer tools to help with these decisions.  So...ATUM DNA2.0  IDT Synthetic gene design made easy GenScript- GenSmart Codon Optimization References Athey...

  19. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...s lab performed a series of positive selection screens in bacteria to identify mutants of S. pyogenes ...sequences (Kleinstiver et al. 2015). From these screens, they identified four novel SpCas9 variants with...

  20. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ... and inhibit its own transcription. As copy number increases, RepA transcription is repressed and replication...

  21. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...editing analysis Once the analysis is complete, a new screen appears with a graphical representation of the ...Data button on the bottom right of the analysis screen. ICE analysis details Contributions   The Contributions...

  23. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ..., and a PhD is not always necessary. The job description and the candidate requirements (and maybe also...customers, internal resources and databases are scrutinized, and this information is enriched with your technical...

  24. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...enrichment (SELEX), some 1010 oligonucleotides could be screened for their affinity to a wide range of non-nucleotide... as the aptamer is bound to a protein fluor. Transcribing and translating a protein fluorophore also takes...

  25. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...the results of studies on reducing antibiotic prescriptions for respiratory tract infections. The video ...provide more than enough time for researchers to describe their science, and for viewers to decide if reading...

  26. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...protocols, titering methods, and quality control are described below. AAV Production AAV distributed by Addgene...) are generated from lentiviral preparations (described above) that are subject to a concentration step...

  27. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...replicate—but not always to the same extent. As described in our previous Origin of Replication post, DNA...act as a feedback mechanism to reduce further transcription. Replication is also regulated by the balance...

  28. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...calendar invite over to the r/Science team with a description of our AMA and Joanne’s preferred time to start...receive via email. The cool features are too many to describe here, but I have over 2,000 notes which Evernote...

  29. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ... lines you are using, it is best to start from scratch. Obtain a vial of the desired cell line from a ...Viral Vector Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors Additional Resources...

  30. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...positive control, the internal control vector, was described earlier. Once you are sure your conditions are...come from multiple, independent sources. We'll describe these a bit more below, but the papers cited in...

  31. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...science, a reviewer’s critical comments on your manuscript is a form of (hopefully constructive) conflict...even better. The Thomas Kilmann Conflict Model describes 5 basic approaches to conflict resolution based...

  33. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ... from a gene synthesis company such as IDT or Genscript, and cloning them sequentially into the chosen...combining the AAV and CRISPR-based approaches is described in several recent reviews (Howes and Schofield...

  34. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...difficult and time consuming. GAL4/UAS was first described in 1993 by Norbert Perrimon’s lab and has been...unicellular organisms, they can also be used to easily screen multiple therapeutic compounds in one experiment...

  35. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...) to remove mutated exons from the dystrophin transcript. Unfortunately, the oligonucleotides only modestly...in one experiment, they found the exon-skipped transcript constituted 59% of total dystrophin mRNA, similar...

  36. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...”, “colony”, etc. This will help save time by screening out irrelevant candidates, and, more importantly...importantly, it’ll help you define salary costs. 4. Ascribe a cost structure associated to these tools, skills...

  37. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...2025).In this latest work, the team designed and screened a large library of iGluSnFR3 mutants, identifying... insertion, a combined endonuclease-reverse transcriptase uses homology arms flanking an RNA payload to...

  38. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ... appealing to researchers.  TALENs In 2010, transcription activator-like effector nucleases (TALENs) were... which is a fusion between Cas9 and reverse transcriptase. Using an elongated template + guide RNA called...

  39. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ... but many of these tips apply to all tissues. Subscribe to Viral Vector Blog Posts from Addgene 1. Location...and can immediately undergo replication and transcription. However, with scAAV the effective genome size...

  41. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    .... Bell, Charles C., et al. "A high-throughput screening strategy for detecting CRISPR-Cas9 induced mutations...Learn to Use Cas9 Activators Perform Genome-Wide Screens with CRISPR Generate Mouse Models with CRISPR/Cas9...

  42. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...performing several rounds of random mutagenesis screening for enhanced RFP variants. PA-mCherry (E26V / ...photoswitching. In 2008, scientists used mutagenesis screens to find a version of Dronpa that doesn't become...

  43. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ... tested and will help healthcare professional prescribe and identify potential beneficiaries. Collaborative...Collection Pages Plasmid Grant to Aid Cervical Cancer Screening in Ghana Modulate Cancer Signalling Resources...

  44. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...interference, as it can target and degrade select RNA transcripts, or be engineered to conduct direct RNA editing...-Aslan, A., & Marraffini, L. A. (2015). Co-transcriptional DNA and RNA Cleavage during Type III CRISPR-Cas...

  45. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...the CGBE architecture. They conducted a CRISPRi screen to identify endogenous genes that impact CGBE efficiency...•G-to-G•C base editors developed using CRISPRi screens, target-library analysis, and machine learning....

  46. New Antibody Animation!

    Type
    Blog Post
    Published
    Feb. 22, 2024, 3:13 p.m.
    ...different antibody isotypes found in mammals.      Subscribe to Addgene's YouTube channel for more helpful ...

  47. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ... you're designing your next virus experiment. Subscribe to Viral Vector Blog Posts from Addgene Need ...

  48. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    Published
    Sept. 19, 2024, 3 p.m.
    ...every quick tip video on our TikTok! Be sure to subscribe to our YouTube channel for quick tips, protocols...

  49. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...of our Data Team, “Our patchwork collection of scripts evolved into a single, organized pipeline and accelerated...
Showing: 351 - 400 of 612 results