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Showing: 351 - 363 of 363 results
  1. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...two visible blue or magenta chromoproteins (Figure 2) (Liljeruhm et al., 2018). This system is a nice confirmation... polylinker sequences of molecular vectors". Proceedings of the Belarusian State University Series of ...
  2. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...excise the HIV-1 genome from every infected cell and 2. prevent reinfection of those cells. Kaminski et al...target sequence. Kaminski et al. next tested their procedure in HIV-1 infected T-cells to see if they could...
  3. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...as the protocol remains the same.    Figure 2: Microscopy-based antibody experiments should include...considerably, then the high degree of variability in the process could make it difficult to obtain meaningful results...
  4. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...experience in cancer research After graduating with a 2:1 BSc in Molecular Biology (roughly a B average in... controlled. I worked from Standard Operating Procedures (SOP’s) and every item I tested was constantly...the technology is so advanced that most of the procedures in the lab are done with robots. Young scientists...
  5. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...Bachelor's degree Generally a short-term position (1-2 years) Often a gap-year position taken to transition...medical practice, and understanding the scientific process can help us understand how new therapies can be...experience. Previous Addgene blogs have discussed the process that goes into choosing a good scientific mentor...
  6. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...ask new and interesting questions in your own lab. 2. Seek multiple mentors during your postdoc At the ...career and you'll become a better scientist in the process. 9. Avoid burnout The suggestions in this blog ...a diet rich in vegetables, foods with minimal processing, and low in empty calories will make you feel...
  7. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...start selecting and testing systemic capsids! Figure 2 contains a decision tree for selecting a capsid, while... highlighted below the table.     Figure 2: This decision tree can help guide your choice on ...transfer to the adult brain. Nature Biotechnology, 34(2), 204–209. https://doi.org/10.1038/nbt.3440 Goertsen...organs. AAV.CAP-B22 was developed through the same process as AAV.CAP-B10 and shows high transduction levels...
  8. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ... from damage (Abe & Lim, 2024).    Figure 2: A) When proteins (blue) interact with the air-water... air-water interface (AWI) during the freezing process, resulting in sample loss. Ci Ji Lim’s lab found...
  9. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...Single-chain variable fragment (ScFv) Figure 2: Comparison between the IgG antibody and scFv. ...purposes, antibodies can be used to affect cellular processes in vivo, and these are referred to as functional...
  10. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...effective recovery of knock-ins, but did result in 1-2 kilobases of additional sequence being introduced....to study gene regulation during developmental processes, CRISPR/Cas9 allows us to delete sequences and...
  11. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...biology 14.6 (2004): 757-764. PubMed PMID: 15582400. 2. Tomkinson, Alan E., et al. "DNA ligases: structure...amplification using cloned thermostable ligase." Proceedings of the National Academy of Sciences 88.1 (1991...
Showing: 351 - 363 of 363 results