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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 444 results for: Ank

Showing: 141 - 160 of 444 results

  1. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target, but many...018-1591-1 Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F (2017) RNA editing...

  2. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...large formats, such as 96-well microplates, and thanks to our dedicated product and development teams,... shared resource of pipelines for genomic data. Thanks to the flexibility inherent in Nextflow’s design...

  4. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...possible facing the committee and not the whiteboard. Thank everyone for the opportunity to share your research...the opportunity to realize your vision. Many thanks to our guest blogger, Erik Snapp! Erik Lee Snapp...

  5. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ..., One Drive, and G Drive. ‘Big’ data like image banks and sequencing files is likely stored in institutional...reproducibility as not all ELNs are created equal. Many thanks to our guest blogger, Rory Macneil!  Rory is founder...

  6. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...GKPIPNPLLGLDST) has been used as a tag since 1992 (Hanke et al., 1992), and has proven itself a versatile...Report, https://doi.org/10.57733/addgene.l0ulu4. Hanke, T., Szawlowski, P., & Randall, R. E. (1992). Construction...

  8. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...resistance (Figure 2). This MazF-ZeoR cassette is flanked by two direct repeats which can be used for marker...through a second recombination event between the two flanking repeats. In cells where recombination has not ...

  10. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...postdocs in the final ruling was, for a lot of us, frankly quite surprising. Consortia of higher education... changes if we wish to minimize the hurt. Many thanks to our guest blogger Gary McDowell! Gary S. McDowell...

  11. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...support and to monitor user replies, but I can’t thank Joanne enough for all the work she put into this.... Although I didn’t start answering until 5pm, frankly, I was typing far before five. Fortunately I have...

  12. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...or virology without acquiring materials from this bank. I am often asked, “What is the difference between...their identity. For example, perhaps a working cell bank has been made by a research institution (an excellent...

  13. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...staff. Many thanks to our guest blogger, Stephanie Hays. Stephanie would like to additionaly thank Isaac Plant...

  14. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...the addition of gene-specific 1 kb homology arms flanking a FLOXed neoR gene. pAAV-SEPT-Acceptor, the preferred... to deposit any new AAV tools you create. Many thanks to our guest blogger, Todd Waldman!  Todd Waldman...

  15. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...transfer plasmid encodes your insert of interest, flanked by long terminal repeat (LTR) sequences, which ...Mansour, M. R., Schwarzwaelder, K., Bartholomae, C., Hubank, M., Kempski, H., Brugman, M. H., Pike-Overzet,...

  16. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ... there is at least ~200 base pairs of sequence flanking the editing site on either side of the amplicon...screening by PCR may be the way to go. Design a PCR to flank your desired edit site and run the PCR product on...

  17. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...Also shown are the “B” restriction sites, which flank the targeted region. Upon PCR amplification, the...minor modifications to a plasmid template. Many thanks to our Guest Blogger Kristian Laursen! Kristian...

  18. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...Technique" in the comments section below! Many thanks to our guest blogger Krissy Lyon! Krissy Lyon is...32. PubMed PMID: 6176866. 3. Hoebeeck, Jasmien, Frank Speleman, and Jo Vandesompele. "Real-time quantitative...

  19. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...Duchenne muscular dystrophy. gRNAs can be designed to flank a single or multiple exons that, when deleted through...low to no off-target activity at the ten highest-ranking predicted off-target sites. Each paper characterizes...

  20. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...Hippocampus labeled with anti-Kv2.1 (magenta), anti-AnkyrinG (green), and Hoechst nuclear stain (cyan). Panels...Immunofluorescence Staining. In W. H. Yong (Ed.), Biobanking (Vol. 1897, pp. 299–311). Springer New York. ...
Showing: 141 - 160 of 444 results