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We narrowed to 452 results for: Ank

Showing: 141 - 160 of 452 results
  1. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...transfer plasmid encoded the desired transgene flanked by HIV-1 long terminal repeats (LTRs) that facilitate...Bernaudin F, Girot R, Dorazio R, Mulder GJ, Polack A, Bank A, Soulier J, Larghero J, Kabbara N, Dalle B, Gourmel...
  2. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...These primers are designed to anneal to sites that flank the insert site. A positive clone will produce a...this type of primer pair anneals to a sequence flanking the insert and one primer anneals to the insert...
  3. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...in-vivo biotinylation signaling peptide (Bio), flanked by hexahistidine motifs (6xHis) MBP Large Protein...Expression Vectors from the Berkeley QB3 MacroLab Eric thanks his wife, Annette Sievers, Ph.D., for her tips ...
  4. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...possible facing the committee and not the whiteboard. Thank everyone for the opportunity to share your research...the opportunity to realize your vision. Many thanks to our guest blogger, Erik Snapp! Erik Lee Snapp...
  5. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...GKPIPNPLLGLDST) has been used as a tag since 1992 (Hanke et al., 1992), and has proven itself a versatile...Report, https://doi.org/10.57733/addgene.l0ulu4. Hanke, T., Szawlowski, P., & Randall, R. E. (1992). Construction...
  6. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target, but many...018-1591-1 Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F (2017) RNA editing...
  7. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...large formats, such as 96-well microplates, and thanks to our dedicated product and development teams,... shared resource of pipelines for genomic data. Thanks to the flexibility inherent in Nextflow’s design...
  8. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ..., One Drive, and G Drive. ‘Big’ data like image banks and sequencing files is likely stored in institutional...reproducibility as not all ELNs are created equal. Many thanks to our guest blogger, Rory Macneil!  Rory is founder...
  9. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...resistance (Figure 2). This MazF-ZeoR cassette is flanked by two direct repeats which can be used for marker...through a second recombination event between the two flanking repeats. In cells where recombination has not ...
  10. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...postdocs in the final ruling was, for a lot of us, frankly quite surprising. Consortia of higher education... changes if we wish to minimize the hurt. Many thanks to our guest blogger Gary McDowell! Gary S. McDowell...
  11. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...support and to monitor user replies, but I can’t thank Joanne enough for all the work she put into this.... Although I didn’t start answering until 5pm, frankly, I was typing far before five. Fortunately I have...
  12. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...or virology without acquiring materials from this bank. I am often asked, “What is the difference between...their identity. For example, perhaps a working cell bank has been made by a research institution (an excellent...
  13. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...staff. Many thanks to our guest blogger, Stephanie Hays. Stephanie would like to additionaly thank Isaac Plant...
  14. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...transfer plasmid encodes your insert of interest, flanked by long terminal repeat (LTR) sequences, which ...Mansour, M. R., Schwarzwaelder, K., Bartholomae, C., Hubank, M., Kempski, H., Brugman, M. H., Pike-Overzet,...
  15. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ... there is at least ~200 base pairs of sequence flanking the editing site on either side of the amplicon...screening by PCR may be the way to go. Design a PCR to flank your desired edit site and run the PCR product on...
  16. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...the addition of gene-specific 1 kb homology arms flanking a FLOXed neoR gene. pAAV-SEPT-Acceptor, the preferred... to deposit any new AAV tools you create. Many thanks to our guest blogger, Todd Waldman!  Todd Waldman...
Showing: 141 - 160 of 452 results