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Showing: 141 - 160 of 332 results

  1. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...biotech industry, which is in the process of capitalizing on phage RBPs as diagnostic tools and therapeutics...between 1 and 3 kb results in optimal likelihood of capturing a whole RBP gene without causing issues associated...

  2. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...after cleavage by the transposase. It has a cargo capacity >100 kB, although integration efficiency decreases...without decreased efficiency, with a maximum cargo capacity of ~200 kB of DNA. Similar to piggyBac, Tol2 also...

  3. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ...pull-down assay, wherein a protein of interest can be captured along with its associated partners. Common pull-down...using antibodies against the target protein. The captured protein complexes are then analysed by mass spectrometry...

  4. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ... cassettes, making them advantageous for small capacity vectors like AAV. In addition to the mammalian...Yang. “Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system.” ...

  5. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...against common tumor markers to enhance cancer cell capture. Fierer et al., PNAS 2014 Apr 1;111(13):E1176...associated with high levels of shRNA expression. The capability to turn RUSH and CRUSH alleles off or on enables...

  6. Viral Vectors 101 eBook 2nd Edition

    Type
    Blog Post
    Published
    July 11, 2024, 1:15 p.m.
    ...elements; resources on different types of vectors, capsids, and tools; and helpful information on everything...

  7. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ...   We love the way the names Aavery, Addy, and Cappy play on viral vector terms. And, the name Kendall...

  8. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ...CRISPR, Addgene gave to me: Selective Endogenous eNcapsidation for cellular Delivery (SEND). On the fourth ...

  9. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...photos and slideshows shared some of the moments we captured over the years, including an album of Addgenie...

  12. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ...haven’t seen our webinar “Navigating an Expanding Landscape for Engineered Systemic AAVs” with the director...

  18. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...nm, photoconverted with 405 nm light, and then captured in the red state after excitation at 561 nm. This...
Showing: 141 - 160 of 332 results