We narrowed to 559 results for: ESP

...Create a monoclonal Cas9-expression cell line. Especially if you’re doing a screen. I made a few monoclonal..., so some of the cells will still appear green despite having active Cas9 (see tip 2). Pro-Tips 1. To ...about 1 month, which I’d say is pretty amazing. Despite the shifting landscape of research opportunities...

...data can be acceptable. However, it is your responsibility to determine what counts as adequate validation...the liver (A). In general, RNA levels tend to correspond to protein levels, so you perform immunohistochemistry...to be present and recognizable by your antibody despite the manipulation. Additionally, not all sample ...

...defined environmental conditions. These methods are especially powerful when coupled with iterative rounds of... antibodies with verifiable data is critical, especially when sharing with other scientists. Addgene partners...synthetic antibody products and development methods. Despite recent advances, there is still always room to ...

...- a major 397 nm peak and minor 475 nm peak, respectively. When wtGFP is illuminated with UV-violet light...I197H) (3) were derived from DsRed and mRFP, respectively. When illuminated with violet light, both variants...field of super resolution microscopy. PA-FPs, especially the monomeric, reversible type, are very well...

...infections. Anything that generates an antibody response in the immune system is referred to as an antigen... is expressed at different times of the immune response and initiates different immune cascades. In the...antibody to recognize. This triggers an immune response within the animal, and its B cells will churn ...

...dyes interact with specific receptors is key, especially for G-protein-coupled receptors (GPCRs). Mutations...also vary in their brightness, depending on the respective fluorochrome. Brightness is impacted by both ...between fluorescent and non-fluorescent states in response to a specific stimuli, which leaves only a small...

... in this post and we disclaim all liability in respect to actions taken or not taken based on any or all...people will have different roles, titles, and responsibilities during their careers - being aware of this...named in the media report (NPR, New York Times, respected online sources) and the media should discuss your...

...systems, naming the new systems PE4 and PE5, respectively. These systems improved editing efficiency by... RT and the S. pombe Tf1 retrotransposon RT, respectively. Their small size comes at the cost of improved...further evolved the Tf1 and M-MLV RT enzymes, respectively, to obtain PEs small enough to deliver with ...

...bacterial phytochrome BphS which produces c-di-GMP in response to far-red light (730 nm). This nucleotide triggers...high-affinity interacting proteins Coh2 and DocS, respectively. The use of far-red light is of particular interest...Zhou et al., 2018). pdDronpa dimers dissociate in response to cyan light (500nm). By fusing the two pdDronpa...

...has been considering a gradual move off X as a response to major platform changes, such as limiting access... and we acknowledge there may be a variety of responses to this decision. However, our decision allows...

...right! He began to study the A. victoria gene responsible for encoding GFP, and, in 1992, he reported its...hypothesized that GFP-like proteins could be responsible and began studying these marine organisms to ...

...advantages of the new system: "First, the light-responsive molecules of cryptochrome 2 are the chromophores...occurs within minutes of light exposure. Third, the response can be graded with light dose and is fully reversible...

...you’d be amazed by the number of out of office responses I’ve received). Clearly we have all of our writers...few successes have trickled into the survey. In response to a bit of a Twitter storm surrounding NgAgo,...

... science as an art form taps into an emotional response within the viewer, which could promote conservation...that we know very little about these specimens - especially those which are now extinct. A collection of ...

...projects, funding opportunities, and community response initiatives. Accelerate the translational impact...COVID-19 research. The new Center for COVID-19 Response and Pandemic Preparedness (CCRP2) at Rutgers University...

...online as one part of their job search. This is especially true of scientists seeking a first job after ...hiring managers to take next steps and follow up especially in large companies. It is fine to address the...

...scientific heroines is always inspiring. I was especially excited to attend this event because Jennifer...they shared to the work they did together. They especially thanked Martin Jinek and Krzysztof Chylinski ...

...efficiency is consistently higher than that of H840A, especially with smaller nick distances. Using D10A, they...strategy can expand the targetable region, which is especially useful if there are no available guides close...

...epigenetic effectors. The diversity of tools is especially exciting, including histone acetylation by p300...and Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9 Day 10: Transcriptional...

...researchers around the world. We take this responsibility seriously. Every decision we make, every project... in the community Addgene fosters a culture of respect, collaboration, and fun Addgene believes there ...