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We narrowed to 557 results for: ESP

Showing: 141 - 160 of 557 results

  1. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...data can be acceptable. However, it is your responsibility to determine what counts as adequate validation...the liver (A). In general, RNA levels tend to correspond to protein levels, so you perform immunohistochemistry...to be present and recognizable by your antibody despite the manipulation. Additionally, not all sample ...

  2. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...position, what will you need? Multiple first or corresponding author publications (published, not in preparation...audiences and if you pay attention to how the audience responds to your presentations, you will become a more ...experience and learn about modern pedagogy techniques, especially active learning. Serve as a daily mentor for ...

  3. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...- a major 397 nm peak and minor 475 nm peak, respectively. When wtGFP is illuminated with UV-violet light...I197H) (3) were derived from DsRed and mRFP, respectively. When illuminated with violet light, both variants...field of super resolution microscopy. PA-FPs, especially the monomeric, reversible type, are very well...

  4. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...infections. Anything that generates an antibody response in the immune system is referred to as an antigen... is expressed at different times of the immune response and initiates different immune cascades. In the...antibody to recognize. This triggers an immune response within the animal, and its B cells will churn ...

  5. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...dyes interact with specific receptors is key, especially for G-protein-coupled receptors (GPCRs). Mutations...also vary in their brightness, depending on the respective fluorochrome. Brightness is impacted by both ...between fluorescent and non-fluorescent states in response to a specific stimuli, which leaves only a small...

  6. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...systems, naming the new systems PE4 and PE5, respectively. These systems improved editing efficiency by... RT and the S. pombe Tf1 retrotransposon RT, respectively. Their small size comes at the cost of improved...further evolved the Tf1 and M-MLV RT enzymes, respectively, to obtain PEs small enough to deliver with ...

  8. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...bacterial phytochrome BphS which produces c-di-GMP in response to far-red light (730 nm). This nucleotide triggers...high-affinity interacting proteins Coh2 and DocS, respectively. The use of far-red light is of particular interest...Zhou et al., 2018). pdDronpa dimers dissociate in response to cyan light (500nm). By fusing the two pdDronpa...

  9. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    Published
    Jan. 11, 2024, 2:15 p.m.
    ...has been considering a gradual move off X as a response to major platform changes, such as limiting access... and we acknowledge there may be a variety of responses to this decision. However, our decision allows...

  10. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 7, 2017, 1:58 p.m.
    ...right! He began to study the A. victoria gene responsible for encoding GFP, and, in 1992, he reported its...hypothesized that GFP-like proteins could be responsible and began studying these marine organisms to ...

  11. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...advantages of the new system: "First, the light-responsive molecules of cryptochrome 2 are the chromophores...occurs within minutes of light exposure. Third, the response can be graded with light dose and is fully reversible...

  12. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...you’d be amazed by the number of out of office responses I’ve received). Clearly we have all of our writers...few successes have trickled into the survey. In response to a bit of a Twitter storm surrounding NgAgo,...

  13. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ... science as an art form taps into an emotional response within the viewer, which could promote conservation...that we know very little about these specimens - especially those which are now extinct. A collection of ...

  14. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...projects, funding opportunities, and community response initiatives. Accelerate the translational impact...COVID-19 research. The new Center for COVID-19 Response and Pandemic Preparedness (CCRP2) at Rutgers University...

  15. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...online as one part of their job search. This is especially true of scientists seeking a first job after ...hiring managers to take next steps and follow up especially in large companies. It is fine to address the...

  17. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...plastic is a substantial portion of lab waste. Despite the fact that researchers represent a minority ... already leading the cultural change toward responsibility in our lab’s environmental impact, but we can...

  18. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...researchers around the world. We take this responsibility seriously. Every decision we make, every project... in the community Addgene fosters a culture of respect, collaboration, and fun Addgene believes there ...

  19. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...protein that undergoes a conformational change in response to the molecule it detects. This change then generates...with luminescent and fluorescent reporters being especially popular. We’ll give you an introduction to fluorescent...

  20. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...often driven by the AAV p5 or p19 promoters to, respectively, produce the large (Rep78 and Rep68) and small...gene which differ between the AAV serotypes are responsible for serotype-specific differences in tissue and...
Showing: 141 - 160 of 557 results