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We narrowed to 565 results for: FIE

Showing: 141 - 160 of 565 results

  2. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...contributed by guest blogger, Rachel Rubinstein, a field-based software product manager at Oxford Nanopore...takeaway themes and customer pain points we’ve identified. I can’t wait to send it out. 4:00 PM--Respond...Rachel Rubinstein! Rachel Rubinstein is currently a Field-based Product Manager at Oxford Nanopore Technologies...

  3. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...how they felt when they read papers outside their field, the answers were primarily negative. Stupid, overwhelmed...highlight the technical details of collecting the purified AAV–that the interfaces between layers are blurred...virions are captured at the 40% layer. To collect purified AAV, insert a syringe at the interface of the ...

  4. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...targeting. Fast forward a decade, and the CRISPR field has exploded with dozens of Cas enzymes and variants...of the enzymes documented that should narrow the field for a candidate Cas for your experiment. Target...search option. For the former, you simply type in identifier keywords (Cas name, RefSeq ID, etc.) and you ...

  5. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...fluorescent reporter. Lastly, once a mutant is identified, the PB transposase can easily and seamlessly...overcome these issues the Denham lab developed a modified monomeric red fluorescent protein (RFP), named...Chen M, Kjems J, Febbraro F, Denham M (2018) A Modified Monomeric Red Fluorescent Protein Reporter for...

  6. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...identifying protein-protein interactions, but RaPID identifies RNA-protein interactions. Both methods rely on... not well characterized RNA motifs. This bait identified a new interaction: binding of the RC2H1 protein...what host proteins interact with Zika’s UTR and identified QKI, an RNA-binding protein that’s highly expressed...

  7. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ..., blue, and green channels overlaid on a bright-field image. Image from Ma et al, 2016. Using dCas9... the signal to noise ratio of the system, they modified GFP-dCas9 and the gRNA scaffold to enhance complex... with a number of limitations. Since color is specified by PAM sequence, each color requires a different...

  8. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...tested this system in vitro and in vivo. They identified cells from 19 genera across four phyla that have...conjugation in vivo studies. However, they only identified seven genera in in vitro experiments, possibly...Clostridium difficile. As mRNA expression cannot be identified with DNA-based qPCR methods, the ability to detect...

  9. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...To solve this problem, gRNAs can be chemically modified to resist degradation while still effectively ...crRNA molecule (usually 1-3 of the first 3 bps are modified) for optimal stability and efficacy.  Base modifications...Hendel, A., Bak, R., Clark, J. et al. Chemically modified guide RNAs enhance CRISPR-Cas genome editing in...

  10. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...activation or gene editing. The more recently identified Cas12f systems from uncultivated archaea are ...almost 40%. The activity of Cas12f1 with these modified gRNAs is comparable to that of SpCas9. The group...group also confirmed that the Cas12f1 and modified gRNA were effective at genome editing when delivered...

  11. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...finding solutions to some of the problems that were identified last year.  In particular, at the symposium we...How many graduates and postdocs – and from what fields – are needed to adequately supply these demands...the number of graduate students accepted in STEM fields (6). Melanie Sinche is a STEM career counselor...

  12. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...recombinase can be expressed only in specified cells or at specified times. Combining this with some of ...well-established research tool, especially in the field of mouse transgenics. A few of the most common uses...

  13. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...website, the new version of which will feature a simplified user interface, expanded numbers of curated molecules...expanded set of links to datasets and reagents in the field. Additional information can be found in the Datasets...The great progress that has been made in the NR field over the last thirty years or so would not have ...

  15. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...delivering shRNA or miRNA. The ever expanding CRISPR field makes wide use of retroviral delivery systems, especially... recombination events and is widely used in the field of mouse transgenics. Experimental uses include:... most recent developments in the genome editing field. Lentiviral Plasmid Kit Multiple Lentiviral Expression...

  16. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    Published
    May 24, 2018, 1:20 p.m.
    ...plasmids all have a PR-T4 vector backbone, which is a modified version of L440. This vector is versatile in that...using synthetically made RNA (riboprobes) with modified nucleotides (for example, Digoxigenin-labelled... to purify the dsRNA from bacteria and mix the purified dsRNA with liver paste, and feed the mixture to...

  17. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...the need for open science and collaboration. The field already has comprehensive reference pages and curated... resources and tools. That message was fully exemplified in Cori Bargmann’s keynote speech and in the ...elegans (Zhang et al., 2015). In this system a modified TIR-1 protein can degrade degron-tagged proteins...

  18. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...Lacy In 2022, Atsushi Miyawaki and colleagues identified a highly bright and stable fluorescent protein...lab generated a series of StayGold mutants and identified a triple mutant, Y187F, R144I and T155Q, which...brains.  Systemic gene delivery capsids previously identified in rodents have not been widely successful in...

  19. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ... has been a breakthrough for the genome-editing field and the greater scientific community. In 2014, we...organoids. This work, and many others, pushed the field forward to where it is today. Below we will review...of using the patient’s own cells that have been modified ex vivo, previously edited healthy donor cells...

  20. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ... Chemogenetics has become a popular tool in the field as it provides a noninvasive method for manipulating...target activation by ligands. PSAMs themselves are modified ligand binding domains. When coupled to the ion...for clinical use. Varenicline has been further modified to create ultrapotent PSEMS (uPSEMs) that have...
Showing: 141 - 160 of 565 results