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Showing: 141 - 160 of 327 results

  1. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...are required for the production of lentiviral particles.  The envelope plasmid typically encodes the vesicular...methods for titering IDLVs include detecting vector particle components or performing quantitative PCR titration...

  2. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...just be a career opportunity. The focus of this article will be talks for non-academic settings. Many of...talk for an academic position, see this very good article at NatureJobs: Interviews: The All-Important Job...

  3. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...is probably in the thousands! The goal of this article is to provide enough background for you to distinguish...Mammalian Vectors Looking for More Plasmids 101 Articles?  Find them all at our Plasmids 101 Topic Page...

  4. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...then, is this: It’s the optimal number of viral particles per volume required to express the gene of interest...spinal cord following injury. Gene Therapy, 26(5), Article 5. https://doi.org/10.1038/s41434-019-0075-6 Issa...

  5. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ... conformation of the plasmid monomer (see this article from BiteSize Bio for more information on how different...linear and nicked plasmids, as described in this article.   Long-read sequencing Addgene uses Illumina...

  7. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...How many times have you ever seen a scientific article attributed to a single author? I’ve seen so few...years and there are currently about 5.5 million articles in the English version of Wikipedia alone. Humans...

  9. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ... require the use of potentially harmful viral particles that express oncogenes, such as Myc. Viral vectors...RNAs are serially transfected using a cationic vehicle. The following synthetic modifications are used...

  10. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...HF-BE3 protein for delivery in ribonucleoprotein particles (RNPs) to both zebrafish embryos and the mouse...list of publications we've highlighted in this article.  Publication Plasmids   Highlights Komor...

  11. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...sequencing. While rAbs are monoclonal, recombinant multiclonal antibodies — mixtures of different rAbs that ...binding; lot-to-lot variability Recombinant multiclonal antibodies Not always available Indirect...

  12. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...also consider using polyclonal or recombinant multiclonal antibodies to increase overall signal from the...antibody binding sites Polyclonal or recombinant multiclonal antibodies Protein not properly lysed Different...

  16. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ...cells and study their effects. To date, nearly 900 articles cite the use of pMD2.G obtained from Addgene. ...

  20. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...how the glycoprotein transforms itself to escape vesicles within host cells and turn the cell into a veritable...
Showing: 141 - 160 of 327 results