...science policy community is the one that was consistently welcoming. This bears repeating. Individuals... early career researchers who have decided to transition out of academia from graduate school or a postdoc... came to DC to do either a sabbatical year or transition away from academia as tenured professors. There...Day One Project for some example proposals and consider applying for their accelerator for some hands-... to future positions, but will also make your transition from academia to the policy world much smoother...smoother.  Action Items:  Explore this comprehensive resource developed by Dr. Kelly Singel which provides...

... This post will go over some of the general considerations you should review before using an antibody....necessarily mean that you yourself have to perform extensive validation experiments for every antibody that...data can be acceptable. However, it is your responsibility to determine what counts as adequate validation...methods. These approaches have relatively low sensitivity, which can be problematic if you are trying to... your antibody’s trustworthiness. You may also consider sharing your validation attempts by other means... test an antibody (perhaps one from Addgene!), consider sharing your results so that others in the community...

...someones) taking on these responsibilities on a team to which you belong, consider stepping up. Otherwise... function. When a select group of people are responsible for a group of specific processes, they must ...tasks done. At Addgene, the Scientist Team is responsible for guiding new deposits into the repository,...roles they’re meant to fill and what their responsibilities are. That’s a subtle but important distinction...- and really can’t - be done alone. So, if you consider yourself part of the scientific community, get...

...aspects of the subject you're writing and then condensing that and making it accessible to students.” Making...for example, cutting open a heart to look at the inside. We do a lot of interactives and videos, as well...specimens.” And since the eScience warehouse is onsite, Caitlin can visit the operations team to make ...curriculum, but has taken on other new and exciting responsibilities.“I’d say maybe 25% of my day is still what...lab: write!” Caitlin did this by editing 15+ comprehensive exam proposals, a few PhD theses, and occasionally...

...multiple high-profile publications. My classmates consisted of graduate students, post-grads, and early career...topic by the moderators. The assignments, which consisted of reading that class’s section of a manuscript...given the opportunity to sign up for a writing intensive weekend in Washington D.C. at the ASM headquarters...Four ways to improve your next manuscript 1. Be consistent with your figures The figures of a manuscript...and they should also be easily understood and consistent in design and layout. 2. Be concise in your writing...

...will discuss some of the features you may want to consider as you make your choice. The first and most important...selection marker or antibiotic resistance. Next, consider the expression level of your construct: would ...pcDNA3-EGFP). If you can get away with generating transiently transfected cells, you will not need a mammalian... lentiviral or a retroviral backbone. This is considered to be a more robust expression system and has...are several types of yeast expression vectors. Consider your applications and experimental design. You...

...society 6)   Consider participating in a future SciFund Challenge. While the goal is ostensibly to get some...magazines or newspapers or other places online, and consider how those stories are being told. Twitter can ...science blogs written by practicing scientists. 3)   Consider starting up your own twitter feed or talking about... both your plasmids and your research stories. Consider practicing communicating your research by guest...

...know all about antibiotic resistance genes, let’s consider another basic element of any plasmid: the origin... intend to use, and whether or not you need to consider your plasmid's compatibility with one or more ...copy number varies. See below for additional considerations. *Requires pir gene for replication (reference... external factors contribute as well. These considerations are especially useful to keep in mind if you...affect copy number. Some ori's are temperature sensitive; others ori's can be "tricked" into amplifying...

...University of North Carolina (plasmids at Addgene). It consists of two domains, LOV2 and Zdk1, where Zdk1 binds...attract other proteins involved in their growth and extension. Now, they split EB1 into two halves (Fig. 1):...RNA localization in mammalian cells. These tools consist of cobalamin-binding riboswitches that bind to ...single base-editors has resulted in the rapid expansion of the genome editing toolkit. However, a challenge...quantitative editing assay. To help accelerate the expansion of genome editing tools, the Reuben Harris lab...

...The light intensity used to activate the PA-FP should also be considered. If the light intensity needed ...your experiment, there are several parameters to consider, such as brightness, photostability, pH stability...protein aggregation. Another important parameter to consider is the contrast ratio- a comparison of PA-FP brightness...microscope will have it’s own quirks (level of zoom, intensity, possible excitation lasers) it is also important...

... donor and acceptor fluorophores, it’s best to consider these cases separately when talking about the ...the tools that are available. Let’s consider the donor first where labeling specificity is most important...AnapRS Mammalian Cells Note: This is not an extensive list Acceptor labeling: Sometimes it's okay to... (i.e. >100 Å). Researchers have developed an extensive array of chemical labeling strategies suitable...understanding of your protein that can lead to novel insights about its biology and its behavior. See the table...covalent labeling of recombinant protein molecules inside live cells." Science281.5374 (1998): 269-272. PubMed...

...conformational changes lead to an increase in fluorescence intensity from the inserted GFP. After characterizing iGluSnFR...by glutamate, and tweaking it to improve its responsiveness, they showed that iGluSnFR could detect neuronal...and produces stronger fluorescent signals upon sensing glutamate in mouse and ferret models. Furthermore...recently made iGluSnFR3 variants that are more sensitive and better localized for synaptic glutamate response... and produce stronger fluorescent signals upon sensing glutamate using both 1-photon and 2-photon imaging...

...from these sites. The Zhang lab generated a comprehensive library of each unique TF open reading frame...cellular fates were then matched with candidate TFs responsible for that differentiation.   Fig. 2: Single...Previously engineered TF libraries were not as comprehensive as this one, so it is an invaluable tool to ... the 24 TFs they tested, 22 expressed markers consistent with the cell type that was predicted based on... if the differentiated cells had morphologies consistent with the predicted cell type (e.g., axon-like...

...personalized steps like “check drawers for light-sensitive reagents” or “re-sterilize now.” Have a physical...Soon you’ll figure out what prompts you need to consistently and accurately run a protocol.   Figure...experiment than it is to rerun an experiment, so consider your prep an investment in accuracy.  Bench setup...hasn't. Developing simple habits like these can consistently reduce errors as you work, and the possibilities... to a protocol if you’re in a hurry, but let’s consider it this way: When you’re stressed or rushing, ...

...can be engineered to be cell type specific. Once inside the host cell, the virus unleashes its cargo – ...signals which can traffic their viral particles inside the nucleus, without the need for envelope disassembly...viruses do. They are generally used to achieve transient, high expression of the relevant cargo without...Capacity (>8kb) High Transduction Efficiency Transient Gene Expression High Immunogenecity AAV ...Immunogenicity Infects Non-Dividing Cells Transient Gene Expression Low Tropism for Mouse Cells and...

...DNA packed into our chromosomes is very much 3-dimensional, as you can see by this post’s chromatin illustrations...PIs, articles, genes, and blog posts are not comprehensive, so just because you do not see a name or gene...the gene you’re looking for on this list, please consider searching directly on our website. In-exhaustive...Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive immunohistochemical reagents. Yamagata M, Sanes...chromatin and the epigenome. If you have been considering some new techniques, the posts below may steer...

.... The next insight was to assemble a synthetic transcription activation complex consisting of multiple...transcriptional activation of endogenous genes and it consists of three components: 1. A nucleolytically inactive...-mediated screens exhibited a high degree of consistency and validation with >80% effectively enriched...optimize humans for CRISPRi and CRISPRa.” Before considering the use of pooled libraries in your experiments...

...They all found the support of the group to be intrinsic to their success. Their stories made it clear ...programs. The groups in these programs typically consist of 1-2 mentors and 3-5 mentees who meet together... of people who are all interested in making a transition. Schedule your first meeting and get organized...in a rotation. The leader for that meeting is responsible for choosing a topic and leading the discussion... your successes and support each other as you transition to the next stage in your careers! Stay in touch...

...channelrhodopsin used as a sensitive fluorescent voltage indicator with near-complete desensitization in continuous... the imbalance of electrical charge between the inside and the outside of the cell. This imbalance is ...Q239 - A GEVI based on the fusion of the voltage sensing domain of a Ciona intestinalis voltage sensor and...anion channel. It is triggered by a short low-intensity blue light pulse and stays open for a longer duration...

...secondary considerations to validation, application specificity, and experimental considerations - no other...has been validated in your specific application. Consider also that there may be variations within an application... your needs.    Multiplex experiments Finally, consider whether you will be using other antibodies in ...from a vendor that you use frequently, with a responsive and knowledgeable technical support team, is ...