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We narrowed to 362 results for: Numb

Showing: 141 - 160 of 362 results

  2. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...within a given sample is simply determined by the number of bands present on the strip. This type of readout...provide a CRISPR-based platform on which an infinite number of tests” for biosensing can be built upon. Mammoth...

  3. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...allowed us to scale our lab work as we grew. As the number of deposited plasmids increased, the accompanying...testing and refinements, we specified a select small number of input file names or filtered the input channel...

  4. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...and state that it would help you manage a large number of files as part of the job. Structure of a cover...adjective that describes you] scientist with over [number] years of experience in [field of research], and...

  5. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...arabinose-inducible promoter pBAD and a low-copy number origin of replication (p15A) worked best under ...Stabilizing the gvpA transcript with WPRE reduced the number of copies of gvpA needed to increase expression...

  6. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ... be unstable and lack precise control over copy number. MUCICAT system contains 4 plasmids: pDonor, pTnsABC...transposition-inducing agar lead to higher cargo copy numbers. (D) The effect of gene dosage on N-acetylglucosamine...

  7. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...time called sprints. The team is mindful of the number of stories we commit to for each sprint, to ensure...the feature. For example, we recently reduced the number of steps involved during checkout. Demo details...

  8. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ..., according to the NSF data) and therefore the numbers of postdocs at different points on this salary ... issues. In the long term, it seems likely the number of postdoc positions in the biomedical enterprise...

  9. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...culture post thaw. In general, the higher the passage number of a culture the lower the transfection and transduction...with other envelope proteins may have a limited number of targets and therefore titers obtained in one...

  10. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...condensed methods descriptions, legends and limiting numbers of figures such that many have multiple, complex...tables in the paper are in scope. For images, the number of examples supporting the published material is...

  11. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...consuming and expensive process. Consider the vast number of possible primary antibodies that exist and you...your protein of interest. Thankfully, there are a number of websites compiling antibody data that can help...

  13. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...expression significantly reduced the HIV-1 copy number, although the efficiency differed from 48-100% ...similar experiment, CRISPR reduced the HIV-1 copy number by over 50% in isolated CD4+ T-cells from two HIV...

  14. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...for an individual application is dependent on a number of factors, including species, strain, age, sex...off-target expression, since they are “seeing” a great number of cells as they circulate in the blood, rather...1011 vg/mouse), AAV-PHP.eB can transduce a greater number of neurons in the CNS (Chan et al., 2017). AAV-...

  15. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...Bradbury & Plükthorn, 2015).  To combat this, a number of groups have come together over the past few ... Strategies for Antibody Validation There are a number of resources available that provide in-depth discussions...

  16. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...they make a copy of themselves. This increases the number of copies of the TE while also increasing the size...TTAA for Sleeping Beauty, they can insert at any number of these sites found in the genome.  To target ...

  17. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...the plasmids in the library in a single tube. The number of unique plasmids in a pooled library can range...us at [email protected]. Include your deposit number and which data needs to be updated. When your plasmids...

  18. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...etc.) can effect reprogramming rates and such a number maybe misleading. Integrating iPSC delivery methods...robust and efficient generation of iPSCs is the number one priority, with safety taking lower priority...

  19. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...you run the plate.    Analyzing There are a number of methods available for analyzing qPCR data, most...Shaun S. LightCycler qPCR optimisation for low copy number target DNA. Journal of Immunological Methods 270...

  20. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ... effective at generating plasmids with varying numbers of repeated sequences or inserts, which can be ...authors optimized these systems by: 1) decreasing the number of plasmids required for each input/output system...new origin of replication increases plasmid copy number in E. coli. The authors used pXPG to study the ...
Showing: 141 - 160 of 362 results