We narrowed to 221 results for: PORA

1. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

   Type

   Blog Post

   Published

   March 3, 2020, 2:15 p.m.

   ... Eisenhoffer lab, is a new tool that increases temporal and spatial precision of gene expression and reduces...

2. Human Germline Editing Using CRISPR

   Type

   Blog Post

   Published

   Aug. 10, 2017, 2:19 p.m.

   ...containing Cas9 and two different gRNAs, and electroporated them with an ssODN repair template in induced...

3. Lighting Up Cell Signaling with Photoswitchable Kinases

   Type

   Blog Post

   Published

   July 25, 2017, 1:20 p.m.

   ...control of kinase activity can provide greater spatiotemporal resolution than pharmacological or genetic ...

4. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

   Type

   Blog Post

   Published

   Aug. 5, 2021, 12:15 p.m.

   ...in pMinDisplay (PDGFR anchoring domain), in electroporated in rat hippocampal cultured neurons, imaged...

5. To Each HIS Own

   Type

   Blog Post

   Published

   March 2, 2023, 2:15 p.m.

   ...tended to be very large and often required the incorporation of a cleavage site and a post-purification cleavage...

6. Transferable Skills Guide: Cross-team Communication

   Type

   Blog Post

   Published

   Nov. 21, 2019, 1:53 p.m.

   ...different ways depending on the team. When Tony, our corporate counsel, used the word “service,” did he mean ...

7. Educational Planarian Tools for Teaching Developmental Biology Techniques

   Type

   Blog Post

   Published

   May 24, 2018, 1:20 p.m.

   ...Hybridization (WISH) WISH is a technique that detects the temporal and spatial localization of mRNA transcripts in...

8. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

   Type

   Blog Post

   Published

   May 22, 2018, 1:38 p.m.

   ... is particularly interested in strategies to incorporate research into undergraduate courses and in communicating...

9. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

   Type

   Blog Post

   Published

   May 15, 2018, 12:43 p.m.

   ...while also capturing information about spatial and temporal organization. The design use to detect PKA activity...

10. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type

    Blog Post

    Published

    July 23, 2020, 1:20 p.m.

    ...pair to another base pair at a specific site. 2. Temporal, spatial, or conditional control of CRISPR activity...

11. Plasmids for Endogenous Gene Tagging in Human Cells

    Type

    Blog Post

    Published

    April 6, 2017, 1:02 p.m.

    ...and the donor plasmid into the cells we use electroporation, a technique that briefly destabilizes the ...

12. Plasmids 101: The Wide World of Natural Plasmids

    Type

    Blog Post

    Published

    Oct. 8, 2024, 1:15 p.m.

    ...plasmids — more on that in a minute! Fun fact! Electroporation is one popular method scientists use to promote...

13. Hot Plasmids February 2024

    Type

    Blog Post

    Published

    Feb. 20, 2024, 2:45 p.m.

    ...difficult to genetically edit due to toxicity from electroporation or poor cellular entry with other transfection...

14. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type

    Blog Post

    Published

    Aug. 24, 2021, 1:15 p.m.

    ...HRP-TMB reaction more quickly. Just make sure to incorporate the dilution factor in your calculation from ...

15. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type

    Blog Post

    Published

    July 12, 2021, 1:15 p.m.

    ...different spike protein variants that can be incorporated into pseudoviruses. The lab used these pseudoviruses...

16. Design Tips for Prime Editing

    Type

    Blog Post

    Published

    Jan. 23, 2025, 2:15 p.m.

    ... cellular surveillance. To limit unintended incorporation of the pegRNA scaffold sequence into the genome...

17. Plasmids 101: FLEx Vectors

    Type

    Blog Post

    Published

    April 28, 2016, 2:30 p.m.

    ...in the absence of the endogenous gene. Spatio-temporal expression of optogenetic reporters: Since most...

18. Tips for Titering Your Lentiviral Preps

    Type

    Blog Post

    Published

    March 15, 2016, 2:30 p.m.

    ...the sample regardless of whether or not it is incorporated into a viral particle. Consequently, titers ...

19. Experimenting in an Open Source lab: from CRISPR to Cats

    Type

    Blog Post

    Published

    July 23, 2015, 2:30 p.m.

    ... funded by someone else (KittyBiome has been incorporated as a non-profit entity and so far most of their...

20. How-to: 5 Steps to a Great Panel Discussion

    Type

    Blog Post

    Published

    Oct. 22, 2014, 1:57 p.m.

    ... members before a panel discussion and often incorporate them into the program. I have to admit to inviting...