Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 468 results for: SEC

Showing: 141 - 160 of 468 results

  1. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ... or in vitro transcribed, and changes in gRNA secondary structure can affect editing efficiency. Gao et...? Let us know how it’s working in the comments section! Read About NgAgo User's First Impressions Here...

  2. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ...you have expertise or knowledge. Where do these intersect? Work with resources: Choose books or articles...her mentoring style. Set goals with a mentor: The secret of accountability To make a mentoring relationship...

  3. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...stained dead within 30 minutes of exposure. 90 seconds of illumination were sufficient for 100% effective...required longer exposure times (5 minutes vs 10 seconds) to achieve the same level of killing as Mg-2I-...

  4. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ..., examining relationships between microbes and insects, mammals, and plants, among others. I particularly...conclusion? In this series, there is always another secret."     - Marcy Patrick, Associate Director of Quality...

  5. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ... chemogenetic inhibition Flp expression for intersectional studies And more! Control AAV We’re always...that can be used in combination with Cre for intersectional control of specific neuronal populations. We...

  7. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...microvesicles (approx. 40 - 150 nm diameter) that are secreted from the cellular membrane into the culture medium...AAVs (Schiller et al., 2018). Exosomal vesicles secreted from producer cells also contain cellular macromolecules...

  8. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ...approaches. It’s very easy to think that the methods section of a paper is the one that you need to spend the...improve the structure and transparency of methods sections, including clear information about reagents, and...

  9. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...necessarily via CRISPR/Cas9. This leaves us with the second critique. Absent of a broad public consensus on...including self-driving vehicles, synthetic biology, biosecurity, and the entire 2018 saga of “what’s happening...

  10. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...Weigert, later expanded on Gram’s discovery, adding a second stain, safranin, as a counterstain to help visualize...smear with the safranin counterstain for 30 - 60 seconds. Rinse the slide in tap water until the runoff ...

  12. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...Meetings: For standing team meetings, provide agenda sections for each person to add a bullet or two about what...out the action items. Move them into a separate section at the top of the agenda, and assign each one using...

  13. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...How can protein function be dissected while minimizing confounding secondary effects? An approach described...purification and have been tested in bacterial, insect and mammalian cells. These vectors were designed...

  14. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...used if the overhangs would have stable ssDNA secondary structure — for example, the stem-loop structure...Gibson assembly takes place may limit formation of secondary structures at the ends of fragments. The major...

  15. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...usually added to the media for uptake by the cells. Second, you’ll need tRNAs and tRNA synthetases specific...incorporated into the cells via plasmid. Third, a second plasmid will be needed to introduce the gene of...

  17. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...meaning it is typically used in conjunction with a second vector that delivers gRNA, such as lentiGuide-Puro...useful tips for generating your own lentivirus. Secondly, you can use a LentiCas9-Blast viral prep to create...

  18. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ... the lab Scientists discovered that deleting a section from the lacZ gene (a mutation called lacZΔM15)...β-galactosidase enzyme. Providing DNA encoding this section of amino acids (called the α-peptide) to a lacZΔM15...

  19. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...cells, hijack host cell machinery, and synthesize a second strand of DNA before expression of their cargo ...DNA goes through the inter-molecular annealing or second strand synthesis. In contrast, a scAAV vector with...
Showing: 141 - 160 of 468 results