We narrowed to 478 results for: SEC

... or in vitro transcribed, and changes in gRNA secondary structure can affect editing efficiency. Gao et...? Let us know how it’s working in the comments section! Read About NgAgo User's First Impressions Here...

...you have expertise or knowledge. Where do these intersect? Work with resources: Choose books or articles...her mentoring style. Set goals with a mentor: The secret of accountability To make a mentoring relationship...

...approaches. It’s very easy to think that the methods section of a paper is the one that you need to spend the...improve the structure and transparency of methods sections, including clear information about reagents, and...

..., examining relationships between microbes and insects, mammals, and plants, among others. I particularly...conclusion? In this series, there is always another secret."     - Marcy Patrick, Associate Director of Quality...

... chemogenetic inhibition Flp expression for intersectional studies And more! Control AAV We’re always...that can be used in combination with Cre for intersectional control of specific neuronal populations. We...

...orange-green, and fast growing cells are green. A second colonizer, labelled with a constitutively fluorescent... used to track how one population responds to a second population. Last, Bernach will simultaneously monitor...

...Weigert, later expanded on Gram’s discovery, adding a second stain, safranin, as a counterstain to help visualize...smear with the safranin counterstain for 30 - 60 seconds. Rinse the slide in tap water until the runoff ...

..., zebrafish, and mice. Second Generation iGluSnFR: SF-iGluSnFR In the second generation of iGluSnFR, ...

...microvesicles (approx. 40 - 150 nm diameter) that are secreted from the cellular membrane into the culture medium...AAVs (Schiller et al., 2018). Exosomal vesicles secreted from producer cells also contain cellular macromolecules...

...necessarily via CRISPR/Cas9. This leaves us with the second critique. Absent of a broad public consensus on...including self-driving vehicles, synthetic biology, biosecurity, and the entire 2018 saga of “what’s happening...

...stained dead within 30 minutes of exposure. 90 seconds of illumination were sufficient for 100% effective...required longer exposure times (5 minutes vs 10 seconds) to achieve the same level of killing as Mg-2I-...

...Meetings: For standing team meetings, provide agenda sections for each person to add a bullet or two about what...out the action items. Move them into a separate section at the top of the agenda, and assign each one using...

...How can protein function be dissected while minimizing confounding secondary effects? An approach described...purification and have been tested in bacterial, insect and mammalian cells. These vectors were designed...

...used if the overhangs would have stable ssDNA secondary structure — for example, the stem-loop structure...Gibson assembly takes place may limit formation of secondary structures at the ends of fragments. The major...

...bead.  Using OptoNBs to control cell signaling The second application for OptoNBs takes us into cells: light-based...reporter nanobody fusions Learn more about using the secondary nanobody toolbox for immunodetection Resources...

...usually added to the media for uptake by the cells. Second, you’ll need tRNAs and tRNA synthetases specific...incorporated into the cells via plasmid. Third, a second plasmid will be needed to introduce the gene of...

...meaning it is typically used in conjunction with a second vector that delivers gRNA, such as lentiGuide-Puro...useful tips for generating your own lentivirus. Secondly, you can use a LentiCas9-Blast viral prep to create...

... the lab Scientists discovered that deleting a section from the lacZ gene (a mutation called lacZΔM15)...β-galactosidase enzyme. Providing DNA encoding this section of amino acids (called the α-peptide) to a lacZΔM15...

...spectrum disorder. It is estimated that CTD is the second cause of male intellectual disability after Fragile...leaflets, instead of three) and acute aortic dissections (a tear in the inner layer of the aorta) are ...

...cells, hijack host cell machinery, and synthesize a second strand of DNA before expression of their cargo ...DNA goes through the inter-molecular annealing or second strand synthesis. In contrast, a scAAV vector with...