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  3. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ... with secondary nanobodies conjugated with the desired fluorophores. In figure 4B below, triple colocalization...

  7. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...infect. Finally, the transfer plasmid encoded the desired transgene flanked by HIV-1 long terminal repeats...

  10. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...small with a print statement and tested that the desired data was available so we felt confident we were...

  11. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...most situations.  A slightly different method is desirable if the antibody is expected to bind weakly to ...

  12. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...neurons. However, VSV-G’s broad tropism can be undesired for in vivo studies since there’s the potential...

  14. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ... sparsely-labeled field of view that was more desirable than having many closely-packed labeled cells....

  15. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...unstructured templates and significantly reduced undesired edits (indels, etc.), while PE6c and PE6d were...

  16. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...contains the signal peptide of MBP, followed by the desired nanobody (NB) sequence, an AviTAG, and 6xHis tag...

  18. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...that their codons are more appropriate for the desired expression host. This is known as “codon optimization...

  19. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...they are born, it’s time to screen them for your desired edit. Good news! Remember the primers you designed...

  20. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...can be prevented and we can progress faster to desired clinical endpoints.   Nick Amiss is an ATCC Outreach...
Showing: 141 - 160 of 181 results