...genome editing tool. However, Cas9’s endonuclease activity can be limited by the need for a specific PAM ...are ion channels that can be used to silence the activity of neurons. Earlier this year, the Hegemann lab...valuable optogenetics feature when studying neuronal activity. The researchers identified MerMAIDS from their...

... Chiasson is currently using VAMP-seq to study vitamin K epoxide reductase (VKOR) variants. VKOR is an...assays for measuring human serotonin transporter activity. Unger also plans to use the serotonin receptor...genotype the patient and rapidly test the variants in vitro against different drugs and dosages.  Michal Bernach...

...from the 3’ end, while its native endonuclease activity degrades the displaced strand.   Figure... the nick site, which was expected since the processivity window of PolI is 15-20 bp. EvolvR’s on-target...bacteriophage T7 DNA polymerase increased the processivity of PolI3M, and thereby increasing the editing...

...upon binding of CNO thus showing no constitutive activity. There are an array of DREADDs that activate different...neuroglia, and have different effects on neuron activity (firing or inhibition). AAV preps from DREADD ...chemogenetics and a concise table of DREADDS and their activity in neurons on Addgene’s chemogenetics guide.   ...

... you to share and build support for your ideas, invite others into decision making processes, and even...usually) presenting either to share knowledge or to invite critique onto an ongoing project. So whenever you...Conflict Resolution Transferable Skills Guide: Creativity...

...in cells, and without something to block RNase activity upon cell lysis, RNA is degraded. To effectively...-based solutions that work by inhibiting RNase activity within cells or tissues- they don’t actually chemically...A (1974) The effect of ammonium sulfate on the activity of ribonuclease A. Biochimica et Biophysica Acta...

...the insert. Gateway® technology (developed by InvitrogenTM) is essentially a more modern version of this...CcdB-resistant strain is ccdB Survival™ from InvitrogenTM. Using either DB3.1 or ccdB SurvivalTM, one ...a variety of useful resources and protocols! We invite feedback from all members of the research community...

...ability to post a preprint as evidence of their productivity that can be shared with hiring and review committees...encourage and consider preprints as demonstration of productivity.  Part of an Open Scientific Communication Ecosystem...details that you couldn’t fit on your poster, or invite attendees to read and comment online! Want the ...

...sensors for a diverse set of fat-soluble hormones, vitamins, and dietary lipids. Included in this family are...progesterone, androgens, and estrogens), fat-soluble vitamins A and D, thyroid hormone, fatty acids, oxysterols...transfection platform for assaying transcriptional activity, for example, was a key development in the functional...

...sequence (Green et al, 2014). The second was an in vitro paper-based platform that allowed gene circuits ...more stringent, and it avoided any days of lost productivity waiting for follow-up DNA oligos to arrive. ...parallel. More broadly, we drew on the combined creativity and expertise of the group and yet never hesitated...

...-base sequence. Double mismatches ablated Cpf1 activity, except when they were present in the 3’ end of... as Kleinstiver et al. saw no decrease in Cpf1 activity with 4-6 base deletions at the 3’ end of the target...found that both orthologs show lower off-target activity than that previously observed with SpCas9. Kleinstiver...

...personal identity. We do this through a variety of activities, including creating educational materials and...addition to events that we put on, we are often invited to present our materials or speak about our experiences...our science communication posts Extracurricular activities for a strong science career path Resources on...

...characterized by deficient phosphomannomutase (PMM) activity due to mutations in the PMM2 gene. Videira’s group... Decades ago, other labs discovered that the in vitro functions of 11 different Arg-mutant enzymes could...phenotypes. Developing a rapid test for the functional activity of VUSs will enable more accurate diagnosis of...

...interact.  When this interaction occurs, BirA* activity is restored, and nearby proteins are labeled with...BirA* enzyme. When this interaction occurs, BirA* activity is restored, and nearby proteins are labeled with...proteins Y and Z part ways, BirA* biotinylation activity ceases. After biotin tagging of proteins in both...

...are monoclonal antibodies that are generated in vitro using synthetic genes that are typically expressed...process of antibody selection and maturation in vitro by selecting for antigen binding from libraries ...or mammalian display (Tsuruta et al., 2017). In vitro selection allows scientists to create antibodies...

...Intraperitoneal (IP) injections are given in the peritoneal cavity, or the space surrounding organs in the abdomen...organs. Restraint: scruffing Location: peritoneal cavity Gauge: 25–27 Volume: < 2–3 ml Skill required: intermediate...tail vein) Intraperitoneal Scruffing Peritoneal cavity 25–27 < 2–3 mL Intermediate Intramuscular Mechanical...

...cell-associated (Vandenberghe et al., 2010). For certain in vitro and in vivo applications, you can consider using..., E., Hernandez, A., Boye, S. L., Boye, S. E., Levites, Y., … Golde, T. E. (2020). Utilizing minimally...assay for adeno-associated virus vectors with sensitivity sufficient to detect single infectious events...

... (500 to 580 nm) over the course of 18 hours in vitro (see figure 1B in Terskikh et al.) and even displays...showed that their versatile timer could be used in vitro, in mammalian cells, in C. elegans, and in Xenopus...changes in oxygen, temperature, and pH, but timer activity can be calibrated to these different conditions...

...Plasmids containing gRNA and Cas9 are used in in vitro transcription reactions to generate mature Cas9 ...ribonucleoprotein complexes Purified Cas9 protein and in vitro transcribed gRNA are combined to form a Cas9-gRNA...nor are these methods limited exclusively to in vitro cell culture. The user should review the current...

...to small-molecules: red-shifted spectra, ion sensitivity, photoactivation, etc. These impressive advances...of the genetically encoded tag, the speed and selectivity of the fluorophore attachment, the brightness...William Perkin. His discovery set off a flurry of activity and the majority of the classic small molecule...