We narrowed to 348 results for: abo.4

...conservative estimate. As a graduate student, I drank 3-4 cups of coffee a day, and I had colleagues who were...learn about all the amazing research being done with plasmids. I heard an impressive talk about the use...pulls off such an inspiring, thought-provoking, collaborative event – all while keeping the party going! At...scientists we've spoken with are excited and passionate about making such tools open-access. Though it is time...

...and isolated.  Following a gating strategy Figure 4 shows a gating strategy from a published experiment...are very close to each other.        Figure 4: Gating strategy used in Barlow-Anacker et al., 2017...series of one- or two-marker plots, like in Figure 3 above, until the population of interest has been successfully...  Of course, there are many more things to learn about when diving into flow cytometry data. There’s different... ways to use FSC and SSC, as briefly alluded to above. There’s many variations in how one can gate, select...

...mice via adeno-associated viruses. Nat Biomed Eng 4:97–110 . https://doi.org/10.1038/s41551-019-0501-5...Commun 10: .https://doi.org/10.1038/s41467-018-08224-4  Swiech L, Heidenreich M, Banerjee A, Habib N, Li ...Addgene Blog Learn more about Cas9 Variants and the Expanded CRISPR Toolbox Read about CRISPR enzyme Cpf1 ... and specific? The two AAV strategies described above showed successful target modification, indicating...

...PMID: 19396156. PubMed Central PMCID: PMC3655711. 4. Gronostajski, R M, and P D Sadowski. 1985. “The FLP...and Removed at Persistent Sites In Vivo.” Neuron 89(4): 756–69. PubMed PMID: 26853302. PubMed Central PMCID... on the Addgene Blog Learn More about FLEX-vectors Learn More about Cre-Lox Check out Retrograde AAVs...expression have been developed. Read on to learn more about FLP-FRT, Cre-lox, and how combinations of FLP and...

...might be contextual fear conditioning (reviewed in [4]). Contextual fear conditioning is a behavioral experiment...PMID: 23473324. PubMed Central PMCID: PMC3595120. 4.Mattis, Joanna, et al. "Principles for applying optogenetic...of the important considerations you should think about when developing optogenetics behavioral experiments...scientific research is that the field benefits from collaboration and the exchange of ideas. The rapid spread ...versatility and standardization of genetic tools in the laboratory mouse, most optogenetics is performed in this...

...standardized, disciplined, and open techniques. Chapter 4: Research projects underway in the Ellis lab including...synthetic yeast chromosome. Listen to learn more about Dr. Ellis, synthetic biology, synthetic chromosomes...Additional Resources from the Addgene Blog Learn about the Synthetic Biology Education Nonprofit BioBuilder...BioBuilder Plasmids 101: Golden Gate Cloning Learn About Working at a SynBio Startup in Our Interview with...

... a fresh new friend: Antibody of the Day!  Figure 4: The plasmid, viral prep, and antibody of the day ...straight from the new search bar, but the menu just above it can help you quickly and easily navigate to educational...down? You’ll find Blugene, as well as information about Addgene, our mission, links to our deposit, request...

... viruses in a BSL-2 lab instead of a BSL-3 or BSL-4 lab. For more details, check out this review article... with a deletion-mutant rabies virus. Nat Methods 4:47–49 . https://doi.org/10.1038/nmeth999 Wickersham... also toxic at high concentrations as described above and is inactivated by the complement system in serum...

...: How did you end up becoming a graduate student? 4:50 - 8:00 : How did you approach doing rotations? ...graduate school community to help keep you excited about coming into lab even when things get tough in the...

...description of the experimental conditions.     Figure 4: A screenshot of the materials and methods section... antibody worked under the conditions described above, and a short description of how the antibody performed...

...preferably near the 5’-end) and that also generates 4 bp overhangs. You can then use the Klenow fragment...Science 239 (1988): 487-491. PubMed PMID: 2448875. 4. Xia, Yongzhen, et al. "New insights into the QuikChangeTM... Brush up on Your Cloning Techniques Learn More about Plasmids in Our Plasmids 101 Series Use CRISPR to...

...with the crRNA, (3) three transposase proteins, and (4) the donor DNA. By using a multi-spacer CRISPR array...Using an Engineered CRISPR/Cas System. ACS Synth Biol 4:723–728 . https://doi.org/10.1021/sb500351f Dong C...diverse bacteria with Mobile-CRISPRi. Nat Microbiol 4:244–250 . https://doi.org/10.1038/s41564-018-0327-...CRISPR Featured Topic Page Learn more about Cpf1 Find out about CRISPR Multiplexing Resources on Addgene.org...coli and other bacterial species. Read on to learn about the tools available for bacteria and some of the...that can be used in this way. As described in the above CRISPRi section, the Marraffini Lab published a ...as a collection of organisms, their genes, and metabolic processes, occupying the gut. Many human diseases...

...scheduled for March 4th from 2:30-4:00 pm ET.  You can learn more about the report's recommendations and...

... and you’re venturing into the uncertain terrain. 4. Never trust the first 20-30 bases of a DNA sequencing...and troubleshooting your DNA sequencing results Above (top right corner of this post) is an example of...

...fluorescent under acidic conditions, with pKa values below 4. Examples include mTurquoise2 (pKa = 3.1), tagRFP ... biology 27 (2015): 39-45. PubMed PMID: 26087123. 4. Kumagai, Akiko, et al. "A bilirubin-inducible fluorescent...deleting residues. Only about 10 amino acids can be deleted from the C-terminus and about 5 from the N-terminus...cylinder with a length of 4.2 nm and a diameter of about 2.4 nm (Hink et al., 2000). The complete beta-barrel...based on cofactor binding proteins as described above. The smallest tags are peptides that are engineered...

...SSC-H / SSC-A gate would work just as fine (Figure 4). As H and A scale in proportion (while H and W do...                                                                                                         B.    Figure 4: (A) Using the proportional relationship between FSC-H...lymphocytes in a mouse tumor sample You made it! So much about the theoretical background. Let’s dive into the ...often choose a looser gate here. I am not worried about including irrelevant events in my analysis, as these...population, acquired from the previous gating pipeline, about 53% of my cells are dendritic cells and ~13% are...ve covered in this blog post. First, you learned about the types of scattering that occur when a cell is...

... translational. Ex: the discovery of GFP. Chapter 4: Goals of the Center for Regenerative Meidince - Understanding...promote scientific sharing. Listen to learn more about Darrell, his research, and his focus on open science...Medicine and in the Department of Pathology and Laboratory Medicine. He attends in the Medical Intensive...NIH-funded Principle Investigator in the Kotton Laboratory and is the founding Director of the Center for...Podcast.     Additional Resources from Addgene Learn about Retinal Gene Therapy from Connie Cepko Find Plasmids...Plasmids from the Kotton Lab Learn more about Stem Cells ...

...mechanisms that cause plasmids to be incompatible. 4. RNA Extraction Without A Kit by Leah Schiesow We... highlighted reagent repositories and other collaborative efforts in the scientific community to speed...store my plasmid in TE, Tris, or water? And what about temperature? Find the details in this blog post....dominates DNA quantification discussions, but what about the other methods to quantify DNA? Here we cover...Incompatibility by Leah Schiesow If you're thinking about hosting multiple plasmids in one cell, it might ...

...to open scientific research data around the world. 4)      The Annals of Internal Medicine published systematic...study probably doesn’t say as much about open access as it does about the state of peer review. 8)     ...University Open Data Access (YODA) Project, “a collaborative endeavor guided by aspirations to promote open...

...targeting Golgi, ER, and endosome membranes) (Figure 4). The authors used SuperPLD to manipulate phosphatidic...fluorescent labeling via click chemistry.    Figure 4: SuperPLD hydrolyzes membrane PC to PA. A) OptoPLD... Check out our Optogenetics Guide to learn more about how these modules work, and read on to learn how...to the cell membrane isn’t powerful enough, what about altering the chemical composition of the membrane...