...accounted for using appropriate replicates and controls (see Tips below), and by validating leads with...reporter gene activation. Including a prey-only control also provides a baseline level of reporter activation...that are known to bind serves as a good positive control to ensure that your setup works. The last thing...

...possible to bring all aspects of our lives under control? Balance is a myth and should not even be the real... don’t have children, so my life will be under control.” Think again. Maybe you’ll need to care for an...to do most tasks – try to resist the impulse to control by insisting things always be done your way. Be...

...anticipated outcomes? What are your controls? Will these controls enable you to interpret your results...figures are missing? For example, are additional controls necessary? As you build your story, are additional...Medical Institute. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum...

...sometimes observe something odd in our uncut DNA control: a band or two appear on an agarose gel at notably...agarose gel Running undigested DNA on a gel as a control when performing a diagnostic digest is a simple...sequencing of our plasmid samples during our quality control process. Since MiSeq involves sequence assembly.... 2006 Sep;4(8):38-40. Summers D. Timing, self-control and a sense of direction are the secrets of multicopy...

...is. Thankfully, through the use of engineering controls — changes made to the virus that eliminate or ...viral vectors to reproduce only in experimentally controlled conditions, making them far safer to work with...

... A fluorescent probe that releases an internal control Another autophagy biosensor is the GFP-LC3-RFP-LC3ΔG...it remains in the cytosol acting as an internal control. Autophagic flux is estimated by the GFP/RFP ratio...

...function of Your Gene Of Interest (YGOI) in a controlled environment under various conditions. The first...great for overexpressing YGOI in an isolated, controlled environment. In most cases bacterial expression...

...needed and when is it not? What are the proper controls needed to pressure-test your FRET assay to make...molecular distances (see Table). However, proper control experiments are needed to establish the dynamic...These experiments include producing a positive control construct where the donor and the acceptor fluorophore... each other on the same protein and a negative control experiment conducted on donor-only labeled protein...

...membrane protein (Plasmid #141386-LV [+M], negative control) were immunoblotted against Anti-SARS-CoV-2 Nucleocapsid...also immunoblotted against vinculin as a loading control (right panel). Image from Addgene. These antibodies...trans-splicing. A cis-splicing vector served as positive control (cis-ctrl). v1, vector 1; v2, vector 2; BD, binding...

...oligomerization of biotinylated proteins on avidin can be controlled easier than formation of GST dimers. Avi-Tag ... of added biotins per molecule cannot be well-controlled. The lack of site selectivity typically diminishes...

...   Listen this episode of Hot Plasmids! Light controls the activity of a microtubule-associated protein...studying cellular dynamics. The photoinducible control of protein function is more specific and much faster...

...cell free system allows for a relatively simple, controlled “one-pot” reaction capable of reproducing useful... and 5HT sensors New AAV tools to selectively control parvalbumin interneurons from the Dimidschstein...

...sequence. Here at Addgene, we use NGS-based quality control to confirm the sequence of all the plasmids we ...ladder based on the expected band sizes. Always run control uncut DNA to ensure your enzymes are working. When...

...rhamnose- and arabinose-inducible promoters. Tight control of toxin expression is absolutely necessary, because...Proteobacteria. We placed the toxin genes under control of a rhamnose-inducible promoter, though Khetrapal...Channarasappa, and Howard M. Salis. "Translation rate is controlled by coupled trade-offs between site accessibility...

...most important ways to spatially and temporally control gene expression, especially in in vivo models, ...functionally similar to Cre-based Brainbow, but is controlled by FLP/FRT recombinase. When placed under different.... The use of AAV provides spatial and temporal control without the need for germline modification, and...

...Ginkgo Bioworks COVID-19 Plasmid Collection Quality control at Addgene in the midst of COVID-19 We received...meant that we had to revamp some of our quality control processes. For the many other COVID-19 related ...

...growth rates will be higher in treatment versus control, and you think there are four important times to..., they will place special emphasis on positive controls and good sample sizes, concerns that are too easy...

...need to ensure you have a bead-only control alongside your input control. Elute the DNA after washing and...

...team develops methods, performs extensive quality control, generates  educational materials, and provides...details about virus production protocols and quality control can be found on Addgene’s Viral Production webpage...

...The gRNAs targeting EGFP can be used as positive controls for gene editing or gene activation, while the...from your primary goal. Getting these quality-controlled reagents from Addgene can greatly expedite a ...