We narrowed to 151 results for: cycs

1. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

   Type

   Blog Post

   Published

   Dec. 15, 2016, 3:57 p.m.

   ... a lambda red phage, λTetR, that carries the tetracycline resistance gene and the lambda red repressor...

2. 9 tips for a successful postdoctoral experience

   Type

   Blog Post

   Published

   Oct. 9, 2018, 12:21 p.m.

   ...presentations benefit from practice. If you simply recycle slides from the last time you gave a presentation...

3. Typing CRISPR Systems

   Type

   Blog Post

   Published

   March 18, 2025, 1:15 p.m.

   ... depth, the Jennifer Doudna lab maintains an encyclopedia of Class 2 CRISPR systems called CasPEDIA, with...

4. Antibodies 101: Flow Cytometry

   Type

   Blog Post

   Published

   July 20, 2021, 1:15 p.m.

   ...modifications, determining cell health, analyzing cell cycle stages, and detecting specific populations of cells...

5. A Needle in a Base-Stack: Cas9 Structural Biology

   Type

   Blog Post

   Published

   June 4, 2024, 1:15 p.m.

   ...many researchers think the enzyme needs cellular recycling factors to release its handiwork (Sternberg et...

6. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

   Type

   Blog Post

   Published

   Nov. 6, 2018, 1:12 p.m.

   ...harmful mutations More, due to selection from more cycles of proliferation Less Cell types All types Less...

7. CRISPR 101: Multiplex Expression of gRNAs

   Type

   Blog Post

   Published

   Sept. 10, 2020, 11:45 a.m.

   ... separately inducible by L-arabinose and anhydrotetracycline (aTet), respectively. pMAZ-SK contains an...

8. Delivery Methods for Generating iPSCs

   Type

   Blog Post

   Published

   April 17, 2018, 1:37 p.m.

   ...replicates in the cytoplasm independent of the cell cycle. A challenge of using Sendai is that since it’s ...

9. Optogenetics + CRISPR, Using Light to Control Genome Editing

   Type

   Blog Post

   Published

   Sept. 3, 2020, 12:15 p.m.

   ...reversible and repeatable when blue light (450nm) was cycled on-off-on. Using a slit photomask, the researchers...

10. Using AAV for Neuronal Tracing

    Type

    Blog Post

    Published

    Aug. 9, 2018, 1:04 p.m.

    ...cells and to the uncoating step in the viral life cycle. Using AAV for neuronal tracing AAV vectors are ...

11. Troubleshooting and Optimizing a Western Blot

    Type

    Blog Post

    Published

    Sept. 17, 2024, 1:15 p.m.

    ...stripping, since you’ll lose protein with every strip cycle. Troubleshooting the troubleshooting There’s many...