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Showing: 141 - 160 of 419 results

  1. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...requires travel can show that you are self organised, reliable, and can work independently. Practical outdoor...are highly desired, as roles are becoming more reliant on technology, and good computer skills will give...

  2. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...culture and who was extremely helpful since she was familiar with library generation. Also, I now know what... next workshop, I’ll ask myself: What are the familiar parts of this workshop? What do I understand ...

  6. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...envelope, Phoenix-AMPHO (for the infection of mammalian cells.) Using this system, virus is produced in...of retroviruses for efficient gene delivery to mammalian cells using 293T cell-based systems. Curr Protoc...

  7. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...H2B (histone)-GFP reporter into the genome of mammalian cells.  Figure 1: The SRIRACCHA reporter...–355 . https://doi.org/10.1038/nbt.2842 Wen Y, Liao G, Pritchard T, Zhao T-T, Connelly JP, Pruett-Miller...

  9. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...This post, along with a future companion post on mammalian vectors, will catch you up on the core replication...and ability to be used in bacterial, yeast, and mammalian cells.   ...

  10. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ... had been collecting data and documenting the compliance of universities with the FLSA in our online resource... salary raising as the only viable option for compliance. However, 10 days prior to the implementation...

  11. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...We are all oh so familiar with the nuclease Cas9, but how does it stack up to the new Cas enzymes on ...RNA cleavage and is significantly less toxic to mammalian cells than Cas13 (likely due to the lack of collateral...

  12. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...are slightly more efficient and less toxic to mammalian cells (Allen, et al., Frontiers in Genome Editing...RNAs to Enhance CRISPR Genome Editing Systems in Mammalian Cells. Frontiers in Genome Editing, 28 (2021)....

  13. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...phytochrome-like fusion proteins from the plant Arabidopsis thaliana. In the presence of red light (650 nm) and its...zebrafish studied, such as the brain, eye, muscle and epithelial cells, as well as in different stages of development...

  14. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...microbiome species such as Snodgrassella alvi and Gilliamella apicoli. The plasmids could also be transformed...Carlotta, et al. "Metagenomic engineering of the mammalian gut microbiome in situ." Nature methods 16.2 (...

  15. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...an international research team from Norway and Australia worked with stem cells in mice. They appropriately... of cells and tissues—when that information is reliable—is important to ensuring excellence in basic research...

  16. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...Reveal Fitness Genes and Genotype-Specific Cancer Liabilities." Cell 163.6 (2015): 1515-1526. PubMed PMID: .... "Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library...

  18. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ... (budding yeast), S. pombe (fission yeast), A. thaliana (mustard weed), O. cuniculus (rabbit), synthetic... plasmid is intended to be used. Choose from: mammalian expression, bacterial expression, lentiviral, ...

  19. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ... NYN PAMs, but with less efficiency and less reliability. Notably, the SpRY variant didn’t display higher...genome! Want to get your hands on SpRY? Find a mammalian plasmid here and edit on! References and Resources...
Showing: 141 - 160 of 419 results