We narrowed to 154 results for: pcr

1. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

   Type

   Blog Post

   Published

   June 11, 2020, 1:34 p.m.

   ...advantages Mapping the brainDissecting circuits Studying GPCR pathways   Acknowledgements We thank the Bryan...

2. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

   Type

   Blog Post

   Published

   April 23, 2020, 1:15 p.m.

   ...for downstream applications (Berns, 1972). Using qPCR and restriction enzyme based methods, we showed ...

3. New and Upcoming Viral Vectors - December 2019

   Type

   Blog Post

   Published

   Dec. 19, 2019, 2:40 p.m.

   ...for the newest GRAB sensors from the Li Lab. These GPCR based sensors provide the spatiotemporal resolution...

4. CRISPRainbow and Genome Visualization

   Type

   Blog Post

   Published

   Feb. 28, 2017, 3:30 p.m.

   ... gRNAs can be expressed in a single vector, pCRISPRainbow-DONOR1, with dCas9 supplied by a separate vector...

5. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

   Type

   Blog Post

   Published

   Sept. 4, 2018, 12:53 p.m.

   ... in gene expression readouts from assays such as qPCR or microarray. But, aside from facilitating the ...

6. Viral Vectors 101: Voltage Indicators

   Type

   Blog Post

   Published

   Jan. 30, 2024, 2:15 p.m.

   ...Rhodopsin GEVIs Rhodopsin is composed of an opsin (a GPCR) linked to a chromophore and functions as a photoreceptor...

7. Great Results Start with Great Standard Curves

   Type

   Blog Post

   Published

   Aug. 15, 2024, 1:15 p.m.

   ...viral vector production facilities frequently use qPCR with a standard curve to titer their viruses; food...

8. Viral Vectors 101: Preparing Pooled Libraries

   Type

   Blog Post

   Published

   July 13, 2023, 1:15 p.m.

   ...cells.  While Addgene uses a physical titer (via ddPCR) to quality control our viral preps, we recommend...

9. Hot Plasmids - August 2020

   Type

   Blog Post

   Published

   Aug. 18, 2020, 1:15 p.m.

   ... of the new viral preps from recent months: New GPCR activation-based norepinephrine (GRABNE) sensors...

10. Finding nucleic acids with SHERLOCK and DETECTR

    Type

    Blog Post

    Published

    April 16, 2020, 1 p.m.

    ...laboratories. In the case of the COVID-19 pandemic, qPCR can be used to diagnose the presence of SARS-CoV...

11. Quick Guide to All Things Lentivirus

    Type

    Blog Post

    Published

    March 21, 2017, 2:30 p.m.

    ...and the reverse transcriptase activity assay uses qPCR to quantify viral RNA. All of these methods have...

12. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type

    Blog Post

    Published

    March 21, 2024, 1:15 p.m.

    ...key, especially for G-protein-coupled receptors (GPCRs). Mutations in receptor sites can affect the fluorescence...

13. Special Delivery: Fluorophore Targeting for FRET Studies

    Type

    Blog Post

    Published

    July 19, 2016, 2:30 p.m.

    ...used either with plasma membrane proteins (such as GPCRs, ion channels, or cell surface receptors) or with...

14. Antibody Validation for Flow Cytometry

    Type

    Blog Post

    Published

    Sept. 10, 2024, 1:15 p.m.

    ...RNAi sequences, confirmation of RNA knockdown by RT-qPCR, and potentially the need to study protein expression...