We narrowed to 157 results for: pcr

...new optogenetic tool called PdCO.  This is an "optoGPCR" (coupled to Go, so it's an inhibitory opsin!)...

...assays we run with all of our in-stock viral preps: ddPCR, purity testing, sterility testing, and viral genomic...

...advantages Mapping the brainDissecting circuits Studying GPCR pathways   Acknowledgements We thank the Bryan...

...for downstream applications (Berns, 1972). Using qPCR and restriction enzyme based methods, we showed ...

...for the newest GRAB sensors from the Li Lab. These GPCR based sensors provide the spatiotemporal resolution...

... in gene expression readouts from assays such as qPCR or microarray. But, aside from facilitating the ...

... gRNAs can be expressed in a single vector, pCRISPRainbow-DONOR1, with dCas9 supplied by a separate vector...

...Rhodopsin GEVIs Rhodopsin is composed of an opsin (a GPCR) linked to a chromophore and functions as a photoreceptor...

...viral vector production facilities frequently use qPCR with a standard curve to titer their viruses; food...

...cells.  While Addgene uses a physical titer (via ddPCR) to quality control our viral preps, we recommend...

... of the new viral preps from recent months: New GPCR activation-based norepinephrine (GRABNE) sensors...

...laboratories. In the case of the COVID-19 pandemic, qPCR can be used to diagnose the presence of SARS-CoV...

...adhesion molecules, transporters, ion channels, GPCRs, and more.  Figure 1: Workflow for cell-...

...and the reverse transcriptase activity assay uses qPCR to quantify viral RNA. All of these methods have...

...key, especially for G-protein-coupled receptors (GPCRs). Mutations in receptor sites can affect the fluorescence...

...used either with plasma membrane proteins (such as GPCRs, ion channels, or cell surface receptors) or with...

...RNAi sequences, confirmation of RNA knockdown by RT-qPCR, and potentially the need to study protein expression...