We narrowed to 149 results for: pcr

1. Viral Vectors 101: Voltage Indicators

   Type

   Blog Post

   Published

   Jan. 30, 2024, 2:15 p.m.

   ...Rhodopsin GEVIs Rhodopsin is composed of an opsin (a GPCR) linked to a chromophore and functions as a photoreceptor...

2. Great Results Start with Great Standard Curves

   Type

   Blog Post

   Published

   Aug. 15, 2024, 1:15 p.m.

   ...viral vector production facilities frequently use qPCR with a standard curve to titer their viruses; food...

3. Hot Plasmids - August 2020

   Type

   Blog Post

   Published

   Aug. 18, 2020, 1:15 p.m.

   ... of the new viral preps from recent months: New GPCR activation-based norepinephrine (GRABNE) sensors...

4. Finding nucleic acids with SHERLOCK and DETECTR

   Type

   Blog Post

   Published

   April 16, 2020, 1 p.m.

   ...laboratories. In the case of the COVID-19 pandemic, qPCR can be used to diagnose the presence of SARS-CoV...

5. Viral Vectors 101: Preparing Pooled Libraries

   Type

   Blog Post

   Published

   July 13, 2023, 1:15 p.m.

   ...cells.  While Addgene uses a physical titer (via ddPCR) to quality control our viral preps, we recommend...

6. Quick Guide to All Things Lentivirus

   Type

   Blog Post

   Published

   March 21, 2017, 2:30 p.m.

   ...and the reverse transcriptase activity assay uses qPCR to quantify viral RNA. All of these methods have...

7. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

   Type

   Blog Post

   Published

   March 21, 2024, 1:15 p.m.

   ...key, especially for G-protein-coupled receptors (GPCRs). Mutations in receptor sites can affect the fluorescence...

8. Special Delivery: Fluorophore Targeting for FRET Studies

   Type

   Blog Post

   Published

   July 19, 2016, 2:30 p.m.

   ...used either with plasma membrane proteins (such as GPCRs, ion channels, or cell surface receptors) or with...

9. Antibody Validation for Flow Cytometry

   Type

   Blog Post

   Published

   Sept. 10, 2024, 1:15 p.m.

   ...RNAi sequences, confirmation of RNA knockdown by RT-qPCR, and potentially the need to study protein expression...