We narrowed to 219 results for: ttl

1. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

   Type

   Blog Post

   Published

   May 5, 2015, 3:54 p.m.

   ...practice to keep up with the scientific literature a little bit at a time. Searching for literature My go-...

2. Career Coaching for Scientists: Why and Where Do I Find One?

   Type

   Blog Post

   Published

   Jan. 29, 2015, 1:25 p.m.

   ...PhD with An Individual Development Plan Learn a Little about The Academic Labor Environment from the Future...

3. Making CRISPR Plasmids Using Fragmid

   Type

   Blog Post

   Published

   Aug. 29, 2024, 1:15 p.m.

   ... Plasmid pRDB_051 (for stable fly cell lines; attL and attR sequences for genome integration by phiC31...

4. Antibodies 101: Stripping and Reprobing Western Blots

   Type

   Blog Post

   Published

   Aug. 1, 2024, 1:15 p.m.

   ...you are using. Dry nitrocellulose membranes are brittle, so proceed with caution. Unlike PVDF, they can...

5. A Guide to Designing a Scientific Poster: Content, Layout, and More

   Type

   Blog Post

   Published

   Oct. 22, 2019, 12:20 p.m.

   ... communicate your work, so you don’t need every little detail on the board.  Poster layout While it’s ...

6. Viral Vectors 101: Calcium Sensors

   Type

   Blog Post

   Published

   Aug. 10, 2023, 1 p.m.

   ...want to observe?’ This information, along with a little nuance discussed below, will help match your experiment...

7. Hot Plasmids and Viral Preps - January 2021

   Type

   Blog Post

   Published

   Jan. 5, 2021, 2:15 p.m.

   ... to cut, Opto-Cre-Vvd is fast and can cut in as little as 2 hours.  Sheets, MB, et al., ACS Synth Biol...

8. Engaging with science and society at pgEd

   Type

   Blog Post

   Published

   Sept. 20, 2018, 12:17 p.m.

   ... and policy. In this post, I hope to tell you a little more about how I ended up at pgEd, what I do there...

9. Adenoviral Vector Production and Troubleshooting

   Type

   Blog Post

   Published

   June 14, 2018, 11:44 a.m.

   ...vector constructs. It consists of 2 plasmids: a shuttle vector (in which the transgene of interest is cloned...

10. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type

    Blog Post

    Published

    May 24, 2018, 1:20 p.m.

    ...morgani and Phagocata gracillis, the process is a little more complicated. These strains are more picky ...

11. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type

    Blog Post

    Published

    May 15, 2018, 12:43 p.m.

    ... localizes primarily to the cell body with very little signal detected along the neurite (Figure 1.) Moreover...

12. Lab Automation at Addgene

    Type

    Blog Post

    Published

    March 22, 2018, 1:28 p.m.

    ...putting some work into finding robots that’ll take a little labor off our hands. Good news! There is probably...

13. Illuminating Epigenetics with A FRET Based Biosensor

    Type

    Blog Post

    Published

    Nov. 19, 2015, 3:30 p.m.

    ...both copies causes disease because too much or too little gene product disrupts normal function. Angelman...

14. Common Injection Routes in Mice

    Type

    Blog Post

    Published

    Jan. 22, 2025, 9:42 p.m.

    ...injections, intraperitoneal injections require a little bit of practice to ensure the proper injection ...

15. Production of Virus in Insect Versus Mammalian Cells

    Type

    Blog Post

    Published

    Nov. 5, 2024, 8:32 p.m.

    ... days. This means that viral production takes a little bit longer in insect cells. Processing Processing...

16. The Scientific Conference Poster Session: Tips for Success

    Type

    Blog Post

    Published

    Aug. 29, 2019, 12:50 p.m.

    ... difficult part of a poster session, but with a little preparation and a few deep breaths the day-of, ...

17. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type

    Blog Post

    Published

    July 2, 2020, 12:40 p.m.

    ...enriched sequences in the experiment and there was little enrichment of DNA targeted by a negative control...

18. Hot Plasmids: Summer 2025

    Type

    Blog Post

    Published

    Aug. 28, 2025, 1:15 p.m.

    ...and Jennifer Doudna’s labs engineered the tobacco rattle virus (TRV), a bipartite RNA virus, to deliver ...

19. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type

    Blog Post

    Published

    June 18, 2020, 1:15 p.m.

    ...sybodies, against membrane protein targets in as little as 3 weeks (Zimmermann et al., 2020). This selection...

20. Academic vs. Industry Postdocs

    Type

    Blog Post

    Published

    Nov. 13, 2018, 1:22 p.m.

    ...I’ve learned and hopefully make your decision a little easier if an industry postdoc is something that...