... any tags or fusions that are on your insert. Typically you would list any tag or fusion protein in the... pBACKBONE-Flag-hGene If there was also an EGFP fused to the C-terminal of your insert you would list ...

...After testing dCas9 fusions with transcriptional repressors, they found that fusing dCas9 to an Mxi1 repressor...covalently attached to the Cas9-gRNA RNP complex via the fused HUH endonuclease PCV. This increases HDR efficiency...

..., Georgieva et al. examined the effects of GFP fusions to inntags or traditional FLAG, HA, and Myc tags...untagged GFP overexpression, while the rest of the fusions had some negative effect on these parameters. IT5... cell morphology was observed with IT5 and IT6 fusions. In contrast, Flag and HA tags had especially detrimental...

...from the Zhang Lab. It's name, though a little confusing to the uninitiated, contains much of the information...directed by a gRNA. 2A-Puro In this plasmid, SpCas9 is fused to the puromycin resistance gene through a 2A peptide...the 2A peptide on its C-terminus, SpCas9 is also fused to a 3X FLAG tag at its N-terminus. This tag can...

...they identified included gene amplifications and fusions, missense mutations, and indels (Ng et al., 2018...carrying a single gene with a defined mutation or gene fusion. Plasmids carrying the corresponding wild-type ...Mutations in Cancer.” Of all the mutations and fusions represented in this collection, approximately 80%...

...genome editing by fusing Cas9 to a mutated human estrogen receptor. This protein fusion stays in the cytoplasm...vector to create knock-ins of fluorescent proteins, fusion proteins, or transcriptional regulators. In this...

... for Goldengate cloning   Design primers for In-Fusion Cloning Design primers for Gibson Assembly and ...restriction enzyme cloning, Gibson assembly, or In-Fusion cloning.  NEB Ligation Calculator: This tool works... from five different insert:backbone ratios. In-Fusion cloning molar ratio calculators Lab math calculators...

...an exon of a gene to generate a transcriptional fusion. This may disrupt the endogenous function of the...the gene product resulting in a transcriptional fusion, much like promoter traps. While this system does...disrupt the gene it monitors due to transcriptional fusion.   Examples of promoter, enhancer, and gene...

...by using the DNA cutting activity of a Protein A fused micrococcal nuclease (MNase) to specifically isolate... against the protein of interest. The Protein A fused MNase is then added and Protein A binds Immunoglobulin...protein interactions Learn about other types of fusion proteins Study chromatin interactions with CAPTURE...

...biosensors contain a single fluorescent protein fused to a domain that senses the concentration of a given...an example. Many calcium biosensors include GFP fused to calmodulin (CaM) and the chicken myosin kinase... an intracellular or extracellular molecule? By fusing a biosensor to an organelle-specific targeting ...