...study its function is one of the most popular applications of fluorescent proteins. These fusion proteins...proxy for dimerization (Shaner et al., 2008 - supplementary figure C). However, these assays are qualitative...linker sequence short In the early days of GFP applications, many were concerned with steric hindrance by...

...that expresses all desired variants of a protein. Applying a selective pressure winnows the pool down to ...mutations are introduced, and scalability (see Supplementary Table 1 of Wrenbeck et al). It uses a uracil...about the same number of positive clones. See Supplementary Note 1 in Wrenbeck et al for details on how ...

...comparison to a control sample. Southern blotting applications range from confirming the results of cloning...sequence thousands of reads at the same time. Other applications of sequencing, such as confirming the results...replaced these, these techniques still have useful applications and have informed many current techniques. For...

...property across various software providers used to supplement their paper notebooks. Instead, all of the important...might be available as well. In most cases this applies to universities. How are you currently exchanging... Besides this being of crucial importance when applying for a patent for example, it also comes in handy...

... barrier of the endothelium, but they could be applied to study other actin-dependent processes in cells...) and to introduce unnatural head groups for applications like fluorescent labeling via click chemistry...constructs are great for in vitro biocatalytic applications where toxicity doesn’t matter. Plus, for folks...

...strand. While this pathway may not be immediately applicable in DSB-induced genome engineering, it has biological...that can be harnessed by researchers for many applications. Your favorite Cas enzyme, a guide RNA to your...Mouse Geneticist's practical guide to CRISPR applications. Singh P, et al. Genetics 114.169771; (2014)...

...success in basic research, researchers are eager to apply it in a clinical setting. CRISPR is often used for...estimated that exon skipping therapies would be applicable to 80% of DMD patients. Figure 1: Exon...researchers are very excited. This approach may also be applicable to a number of rare genetic diseases caused by...

...et al (2). In this post, I’ll cover two great applications of the NLuc technology. The first couples NLuc...the coupled luciferase. This strategy was first applied using RLuc8, an engineered Renilla luciferase, ...fluorescence. The paper reports a wide variety of applications of the Enhanced Nano-Lanterns including monitoring...

...missing or added at the broken ends and, as such, cripple the gene. However, if repair material is provided... with Biogen and is particularly interested in applying CRISPR for mouse genome editing.     Haoyi Wang...interested in using CRISPR to develop therapeutic applications that better human health.     References Cong...

...piggyBac™ technology can be used for numerous applications, including transgenesis, gene-trap screens, ...technology that may be used for a variety of research applications, including making transgenic cell lines and ...Fingers, TALENs, and CRISPR-Cas9 nucleases. Mathew applies his specialized knowledge to help researchers identify...

...secondary antibodies.   Application-specific controls Some antibody-based applications have very specific ...then moving on to controls for antibody-related applications. After reading, you will have a better understanding...

.... A. (2023). Various AAV Serotypes and Their Applications in Gene Therapy: An Overview. Cells, 12(5), ...Retrograde Serotype Viral Vectors 101: Viral Applications...

...multiplexed IF, many of the same considerations apply as for standard IF. However, there are a few specific...primary antibodies that have been validated in IF applications (find IHC validated or ICC validated antibodies...Immunocytochemistry Protocols for other antibody applications...

...for sphingomonads and other Alphaproteobacteria. Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi...cumate-inducible gene expression system for Bacillus. Appl Microbiol Biotechnol. 2019 Jan;103(1):303-313. doi...expression of genes for metabolic engineering applications in Saccharomyces cerevisiae.” FEMS Yeast Res...

... have been completed. Therefore, to be ready to apply for a Group Leader position, key first author manuscripts... project. Furthermore, Group Leader position applications require 3 letters of reference- the graduate...informal recommendations, more positive grant application and manuscript reviews, etc. You'll also meet...

... be mostly on the brain, but many of these tips apply to all tissues. Subscribe to Viral Vector Blog Posts... infect) can influence an AAV’s value greatly. Applying this mantra to AAV selection, there are three ...only can we compare these methods in specific applications, we may also be able to use these comparisons...

...silencing can be easily induced by exogenous application of long or short dsRNA. However, it is important...have benefited from the ease of use in dsRNA applications. Figure 1: Schematic of an RNAi-inducing..., which is conserved in many organisms. When supplemented with adenine analogs such as 2-fluoroadenine...

...Shakespeare for the first time. The discovery and application of CRISPR-Cas9 kicked off over a decade of frenzied...mechanisms in type I-C CRISPR-Cas3 for genome-editing applications. Molecular Cell, 84(3), 463-475.e5. https://...Mashimo, T. (2022b). Genome editing technology and applications with the type I CRISPR system. Gene and Genome...

...Thinking about using antibody-based applications in your experiment...but not sure where to start or ...or quick refresher on — these common antibody applications.  Thanks for watching, and we hope you found...

...New to the lab? Or looking for ways to supplement your teaching? You’re in luck! Addgene’s Intro to the...techniques. It’s also great for teachers looking to supplement their curriculum. From the details about personal...