We narrowed to 291 results for: Pol

...the surface of the protein where the nascent polypeptide emerges from the exit channel.   He then said...

...HaloTag, SpyTag). The Foerstemann lab developed a polymerase chain reaction (PCR)-based system that allows...

..., Cas9 forms a steric block that prevents RNA polymerase from efficiently transcribing the gene. This ...

...endogenous CRBN E3 ligase complex which will polyubiquitinate the fusion protein. This will target the protein...

...Chemical transfection   Lipid mediated Cationic polymers Calcium phosphate Cells Transformed cell lines...

...Praparaten. Leipzig: S. Hierzel.
 J.A Dent, A.G. Polson, M.W. Klymkowsky. (1989) Development, 105.61-74...

...have approximately 20% more single nucleotide polymorphisms (SNPs) that map to guides in the Broad’s Genomic...

...initiating the whole process – such as the viral DNA polymerase – it makes sense that vectorization strategies...

...intense study. Two methods to accomplish this are Polyethylene Glycol (PEG) mediated transfection of protoplasts...

...resources on the Addgene blog Read more about polymerase chain reaction Browse our blog posts related ...

... these fluorophores are often bulky and contain polar groups (Figure 2b), which can preclude their use...

...has four layers of location which can all be extrapolated from the 2D barcode and LIMS: Barcoded Tube...

...engaging target DNA. The reverse transcriptase polymerizes DNA onto the target strand based on the sequence...

...Split inteins are a pair of naturally occuring polypeptides that, when at the ends of two proteins, mediates...

... psychiatric diseases such as schizophrenia or bipolar disorder. In both diseases, animals have reduced...

..., including investigations into environmental pollutants and health, embryo growth, brain function, and...

...Wieschaus. "Mutations affecting segment number and polarity in Drosophila." Nature287.5785 (1980): 795-801...

...of the causes of culture shock above, but after polling some Addgene colleagues, we’ve come up with a list...

...It’s often easiest to train individuals who are polite, appreciative, and clear about their needs. Remember...

... the cells could be batch-sorted to isolate a polyclonal cell line with strong gas vesicle expression ...

...skill is a hallmark for jobs focusing on: Science Policy, Business Development, Administration and Intellectual...

... from the ori. In the elongation phase, a DNA polymerase synthesizes a new DNA strand at each replication...

...structural elements, oris interact with various RNA polymerases and proteins from bacterial hosts. In a plasmid...

...interest (red) is amplified using Recombinase Polymerase Amplification (RPA) or Reverse-transcription ...

... interact with mediator complexes and the RNA polymerase at the promoter region of a gene to initiate ...

...Paolo, et al. "Systemic errors in quantitative polymerase chain reaction titration of self-complementary...

...the job. When dealing with customers, you must be polite, friendly, patient, attentive, empathetic, and ...

... Marc Sze, Victoria McGovern, and Julie Wolf. Apologies to anyone I’ve inadvertently left off this list...

...whether the cell lines had isoenzymes and genetic polymorphisms specific to their origins. This technique is...

...induction system. The DE3 lysogen expresses T7 RNA polymerase (RNAP) from the bacterial genome under control...