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Showing: 241 - 270 of 515 results

  1. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...made to moderate and cross-check all data with published literature as soon as possible after submission... queries.  Looking for a bright, monomeric FP published in the last few years with an emission peak around...

  2. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...analysis script should return the same output: a spreadsheet of read counts. You can use these counts to confirm...and maintenance of good representation. Addgene wishes you luck in your future library use and screening...

  3. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...do just this.  In under a month’s time, we’ve published an article on BioRxiv describing a comprehensive...tracking their fulfillment manually in a shared spreadsheet and it takes time away from our research. For...

  5. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...sequenced There are several human disease models established in yeast, including a widely used Parkinson’s...recombinant protein its major secretion. Beyond sheer protein production, K. phaffii is exceptionally ...

  6. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...collaborative cloud tools such as Google Docs and Sheets during Zoom lab meetings to chip in ideas and take...collaborative writing and this recent article published in PLOS Computational Biology (Frassl et al., ...

  7. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...why the results you've measured should change established theory. So, what can you do to ensure that your...available. But think of this as a jumpstart or a refresher for your skillset rather than a permanent fix....

  9. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...worldwide. Distributing hardware via globally established makerspace networks and training users via workshops...studying plankton using flow cells, has become an established alternative to high-priced commercial devices...

  10. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...homologue) or extensive DNA synthesis. NHEJ also finishes repair of most types of breaks in tens of minutes...Limiting the Persistence of a Chromosome Break Diminishes Its Mutagenic Potential. PLoS Genet 5:e1000683...

  11. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...tissues. Although antibody production is well established, it’s also a process that’s difficult for individual...their RANbodies, Yamagata et al used previously published nanobody sequences that targeted the following...

  12. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...Note: After this blog was published, a bioRxiv preprint that questions the conclusion of inter-homologue...with a storm of media coverage. But the paper published August 2nd in Nature gives us even more to talk...

  13. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ... kits to make their life easier. Recently, we published a blog on DNA purification without a kit that ...molecules (Allewell and Sarma, 1974). Additionally, ThermoFisher provides a protocol on how to integrate RNAlater...

  14. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...self-assembling protein (SAP) such as a bacteriophage shell protein or a bacterial microcompartment protein....expressing the SAP-RFP fusion. Then, the cells are washed to stop the production of new particles. With subsequent...

  15. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...central and peripheral organs because of their established roles as molecular sensors and governors of endocrine-hormone...the basic assay remains widely used today.  The sheer abundance of these critical research tools is a ...

  18. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...Originally published Apr 30, 2020 and last updated Jul 12, 2021. What started out as a small collection...lab instead of a BSL-3 lab. Jesse Bloom’s lab published a protocol and reagents for pseudotyping lentivirus...

  20. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...across the globe. This post was written by Alyssa Shepard, with significant contributions from Alyssa Cecchetelli... Alyssa Shepard...

  21. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...expanded organoids. This work, and many others, pushed the field forward to where it is today. Below we...because of its potential for allogenic “off-the-shelf” treatment. In short, instead of using the patient...

  22. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...original, though its maturation is even faster. Published just a few days later, Mohan Balasubramanian and...-3188559/v1.  Neurogenetic alchemy: CAP-Mac unleashes the secrets of primate brains with brilliance ...

  23. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ... is fast and can cut in as little as 2 hours.  Sheets, MB, et al., ACS Synth Biol. 2020. https://pubs.acs.org...Expanded serotypes are now available for Gordon Fishell Lab constructs targeting GABA neurons. Serotypes...

  24. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...audience’s eye and draw people in. That can be accomplished with an exciting title and attractive figures...spacing between them so that they are easily distinguished. The UCLA Library offers some Powerpoint based...

  25. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...landscape facing all scientists, both young and established, as they embark on or continue their careers .... and Social Media Content Specialist at Thermo Fisher Scientific Developing effective science advocates...

  26. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ... second key point; the fiber optics need to be polished to significant smoothness to prevent drastic loss...it’s best to try those that have already been published first. Because behavioral optogenetics is performed...

  27. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...well as premixed and ready-to-use reagents. Thermo Fisher Scientific Inc., Origene, and NEB® are all good...you. Happy cloning (and be sure to deposit your finished constructs at Addgene!). References 1. Li, Chaokun...

  28. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ... this is being used, it’s best to turn to an established social media platform. During the conference ...something on social media. If someone is presenting unpublished data they may ask the audience to not share their...

  29. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ... genome that's then packaged into AAV’s protein shell, or capsid; 2) the pHelper plasmid which expresses... only the cultures with intact ITRs. I use a spreadsheet similar to this one to plan my AAV packaging ...

  30. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...reels in the non-target strand of DNA much like a fisherman would reel in a big catch. It was originally suspected... Alyssa Shepard...
Showing: 241 - 270 of 515 results