We narrowed to 321 results for: bre

1. Antibodies 101: Stripping and Reprobing Western Blots

   Type

   Blog Post

   Published

   Aug. 1, 2024, 1:15 p.m.

   ...Β-mercaptoethanol (βME) 100–115 mM = 0.7%–0.8% (v/v) Breaks disulfide bonds HCl Adjust pH to 6.7–6.8 ...

2. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

   Type

   Blog Post

   Published

   May 4, 2021, 1:15 p.m.

   ... Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS (2019) A toolbox of IgG subclass-switched...

3. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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   Blog Post

   Published

   April 6, 2021, 12:30 p.m.

   ....   Confirming tags and fusion proteins. We’ll break down each of these steps below. Aligning the sequence...

4. Hot Plasmids and Viral Preps - January 2021

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   Blog Post

   Published

   Jan. 5, 2021, 2:15 p.m.

   ...Dual-fluorescent DSB Repair) is a double-strand break repair assay for mammalian cells. It's based on ...

5. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

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   Blog Post

   Published

   Sept. 13, 2016, 2:30 p.m.

   ...Howell -Founder of Howell Communications Philip Brenner, PhD - Assistant Professor of Sociology. University...

6. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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   Blog Post

   Published

   Dec. 1, 2015, 3:30 p.m.

   ... Addgene depositor) Craig Mello. Taking his groundbreaking work in RNAi one step further, his lab is now...

7. The Materials Science of Optogenetics Experiments

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   Blog Post

   Published

   Sept. 17, 2015, 2:30 p.m.

   ...location of your region of stimulation (the z-axis bregma coordinate). Because the light that is emitted ...

8. How-to: 5 Steps to a Great Panel Discussion

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   Blog Post

   Published

   Oct. 22, 2014, 1:57 p.m.

   ... follow and gets things rolling. It can make or break the discussion section. And what about those audience...

9. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

   Type

   Blog Post

   Published

   June 18, 2020, 1:15 p.m.

   ... Vlieger D, Corbett KS, Torres GM, Wang N, Van Breedam W, Roose K, van Schie L, Hoffmann M, Pöhlmann S...

10. Plasmids 101: 5 factors to help you choose the right cloning method

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    Blog Post

    Published

    Aug. 21, 2018, 12:31 p.m.

    ...methods that make working with multiple inserts a breeze. Among them are some of the homology based methods...

11. Polymerase Chain Reaction Overview and Applications

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    Blog Post

    Published

    Aug. 10, 2021, 1:15 p.m.

    ...dsDNA template to a point where the hydrogen bonds break between the base pairs. This results in the separation...

12. Degrading DNA with Cascade-Cas3

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    Blog Post

    Published

    Feb. 11, 2025, 2:15 p.m.

    ...During DNA repair, there is a chance that the two breakpoints will repair without the section between the two...

13. Common Injection Routes in Mice

    Type

    Blog Post

    Published

    Jan. 22, 2025, 9:42 p.m.

    ...patience, as you wait for the anesthetized mice to breathe in the liquid. Restraint: anesthesia Location: ...

14. Twenty Years of Addgene Sharing: CRISPR

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    Blog Post

    Published

    April 18, 2024, 1:15 p.m.

    ...effectors. Nucleases generate a classic double-stranded break, while modulators regulate transcription and chimeric...

15. PhD Applications After COVID

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    Blog Post

    Published

    Jan. 5, 2023, 2:15 p.m.

    ...and video communication. But first, take a deep breath; at least you know you have an answer to that previous...

16. Transferable Skills Guide: Managing a Team

    Type

    Blog Post

    Published

    Jan. 30, 2018, 2:42 p.m.

    ...) everything ourselves and it’s a hard habit to break. But as a manager you will be quickly overwhelmed...

17. 3D Printing Meets CRISPR Cas9

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    Blog Post

    Published

    Oct. 17, 2017, 12:55 p.m.

    ...sequence of events plays itself out: The researcher breaks eye-contact with any other human in the room. Their...

18. Truncated gRNAs for Regulating Gene Expression

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    Blog Post

    Published

    Jan. 10, 2017, 3:37 p.m.

    ...binding the protospacer. The resulting double strand break (DSB) stimulates DNA repair mechanisms that can ...

19. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type

    Blog Post

    Published

    April 19, 2016, 2:30 p.m.

    .... However, if the molecules are confined to a subregion of the cell and/or they cannot tumble in 720°,...

20. Experimenting with New Careers while in Grad School

    Type

    Blog Post

    Published

    Feb. 25, 2016, 3:30 p.m.

    ...to get recharged. The fun change of pace helped break up the negativity and allowed me to focus on the...

21. Genome-wide Screening Using CRISPR

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    Blog Post

    Published

    Aug. 18, 2015, 2:30 p.m.

    ...-type Cas9 and a gRNA generates a double-strand break in the target DNA, which, when repaired through ...

22. Light Sheet Fluorescence Microscopy

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    Blog Post

    Published

    July 16, 2015, 2:30 p.m.

    ... the human ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile tract, Cu= Cuneate tract, dCST=...

23. Which Fluorescent Protein Should I Use?

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    Blog Post

    Published

    May 20, 2014, 2:06 p.m.

    ...CFP), and yellow FP (YFP) were derived. For a breakdown of GFP, it's variants, and their relevant mutations...

24. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type

    Blog Post

    Published

    Sept. 6, 2018, 12:02 p.m.

    ...Zhen, et al. "Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes...

25. AAVs in Retinal Gene Therapy

    Type

    Blog Post

    Published

    July 31, 2018, 12:55 p.m.

    .... Jean Bennett (UPenn) that resulted in the groundbreaking 2001 study showing that AAV-mediated delivery...

26. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type

    Blog Post

    Published

    July 1, 2025, 1:15 p.m.

    ...of ~1.8 and an A260/230 of ~2.0. However, let’s break down how different contaminants impact these ratios...

27. Viral Vectors 101: The Retroviral Lifecycle

    Type

    Blog Post

    Published

    July 27, 2023, 1:15 p.m.

    ...tools in molecular biology. “Retroviruses” is an umbrella term which encompasses 1.) the gamma-retroviruses...

28. Getting to Know Expansion Microscopy

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    Blog Post

    Published

    June 20, 2023, 1:15 p.m.

    ...SRM), a family of imaging techniques developed to break through the resolution barrier while still utilizing...

29. Great Results Start with Great Standard Curves

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    Blog Post

    Published

    Aug. 15, 2024, 1:15 p.m.

    ...and the quality of a standard curve can make or break an experiment. Here, we will provide an overview...

30. Transferable Skills Guide: Identifying Your Transferable Skills

    Type

    Blog Post

    Published

    Jan. 11, 2018, 3:29 p.m.

    ...employer. Your heart is pounding, you’re short of breath, and you’re a little jumpy. You are at another ...