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We narrowed to 608 results for: Sis

Showing: 321 - 360 of 608 results

  1. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...using words for their field or technology in a consistent way. Just creating a lexicon was not enough. ... using the same words with the same meanings consistently, meetings will be shorter and more productive...

  4. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...other genetic disorders, pre-implantation genetic diagnosis (PGD) screens embryos to find those that do not...determine its genotype. However, this detailed analysis is not possible with IVF, as most of the cells...

  5. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...antibiotic in the plate correspond to the antibiotic resistance marker present in your plasmid. You should also...is due to a PCR mistake or a mistake by the DNA analysis software. Now that you've read our plasmid troubleshooting...

  6. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...technical details of the problem you're solving, but consistency is key. Some namespace collisions you may encounter...treating these pieces of data in ways that are inconsistent with how they are being treated in the lab, ...

  8. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...presence? Learn a key new job skill? Finish your thesis? Knowing your desired outcomes will help you assess...For example Dora Farkas, founder of Finish Your Thesis (who has also blogged for us) offers a free report...

  9. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...as in important physiological states such as adipogenesis and macrophage activation. Our mass spectrometry-affinity...coregulators in HeLa cells was the first comprehensive analysis of the interactome of these key regulatory molecules...

  10. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...always helpful to remember that if a PI asks for assistance in the grant application process, she has faith...deem your proposal successful. Typical proposals consist of 3 to 5 aims, with secondary milestones to meet...

  11. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...topics that may require additional attention during analysis. These include topics such as: locations in a ...on Addgene.org Educational Resources Sequence Analysis of a Plasmid Protocol ...

  12. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...All destination vectors contain an ampicillin resistance cassette and an origin of replication, which ...pRDB_052 (for stable fly cell lines; Hygromycin resistance gene; for genome integration by spontaneous insertion...

  15. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...fills all those vials? Well, it used to be a lab assistant, but with increased plasmid demand it was too ...fill 400 vials in 35 minutes compared to a lab assisstant filling about 200 vials in 45 minutes by hand...

  17. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...community involved in sampling or bioinformatics analysis or upload your data to Zooniverse. The Natural..., Sarah Schmidt! Sarah Schmidt is engineering resistance against Fusarium wilt disease in bananas as a...

  18. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...fluorescent timer emits blue light around the time of synthesis and, after a few hours, a conformational change...myeloid leukemia and saw that expression of H2B-FT consistently predicted the cell cycle speed across these ...

  19. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ... will be selectable with the antibiotic whose resistance gene is encoded in the viral vector. This antibiotic... may be some low-level fluorescent protein or resistance marker expression following transfection, higher-level...

  20. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...rates, determined using sophisticated genome-wide analysis. At many of its computationally predicted off-... Hur JK, Been KW, Yoon SH, Kim JS. Genome-wide analysis reveals specificities of Cpf1 endonucleases in...

  21. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...rho-independent. Rho factor is a helicase which assists RNA polymerase in the termination of the transcript...: PMC102582. Additional Resources A detailed analysis of yeast terminators of varying strength. A discussion...

  22. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...will be erratic, opsin activation/inactivation inconsistent, and behavioral results impossible to interpret...sequentially increasing fineness helps to maintain a consistent stream of light. Test your implant by connecting...

  23. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ...transferable skills such as leadership skills can only assist you and make you more qualified for future career...blogger Juliet Moncaster! Juliet Moncaster is an Assistant Professor at the Boston University School of Medicine...

  24. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ... increase efficiency of these systems, though consistent expression can still be a challenge. A multitasking...Dolan, A. E., Liao, M., & Ke, A. (2018). Structure basis for RNA-guided DNA degradation by Cascade and Cas3...

  25. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ... easier, and more comprehensive thanks to the consistent development and improvement of CRISPR-based technologies...& Platt, R. J. (2023). Transcriptional linkage analysis with in vivo AAV-Perturb-seq. Nature, 622(7982...

  26. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...the virions out. We recommend using a Tris-based lysis buffer and sonication.  The obtained virions from...can then remove the density gradient material by dialysis/buffer exchange and further concentrate the virions...

  27. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...you can adjust compensation values later in your analysis, unlike voltages.  Figure 1A shows a single-color...always change your compensation values during the analysis portion of your experiment. So, as long as you...

  28. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...requesting each year (Figure 2). We did a similar analysis with CRISPR pooled libraries, although we used... from this data is that CRISPR technology is consistently showing rapid development and quick adoption...

  29. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...compartment was used to optimize D-glucaric acid biosynthesis. This MPyV system encapsulated a rate-limiting... a compact Cas9 ortholog from Staphylococcus lugdunensis that recognizes a NNGG PAM and has comparable...

  31. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...the cell pellet, I lyse the cells with this AAV lysis buffer and freeze/thaw the lystate four times in...-on time and 2 hours for the qPCR run and data analysis. Refer to this Addgene AAV Titration by qPCR Protocol...

  32. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ... popular cloning methodologies, with a special emphasis on these five categories. Please note that many...backbone containing a topoisomerase I cut site, toposisomerase I, and a taq polymerase-generated insert. When...

  34. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...Domain (VSD) GEVIs Simply put, VSD-based sensors consist of a VSD fused to a single fluorescent protein ... Single Fluor Chemigenetic Sensors This system consists of a genetically expressed enzyme, typically on...

  35. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...difficult” GC-rich regions of the genome, which seem resistant to Cas9 cleavage (1). Both the CRISPR-Cas9 and...however rely on cloning into a vector or in vitro synthesis, which could be less desirable if one has to target...

  36. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ... a reporter, such as a fluorescence gene or a resistance gene. These reporters allow you to easily screen...thought to function by making the ribosome skip the synthesis of a peptide bond at the C-terminus of a 2A element...

  37. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...cytosine deaminase. Three rounds of rational mutagenesis on the ADAR2DD residues that contact the RNA ...RNA-sequencing, the authors performed rational mutagenesis and identified that a S375A mutation in RESCUE...

  39. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...advantages: they are convenient and provide uniform, consistent results. But they are also less accessible due...columns from DNA purification kits. Kit-free alkaline lysis plasmid miniprep Starting material: 2 mL bacterial...

  40. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ... HaloTag, TMP-tag) – These widely used systems consist of a genetically encoded enzyme variant tag that...fluorogens (Figure 1e). Stains – These labels consist of a fluorophores conjugated to molecular species...
Showing: 321 - 360 of 608 results