We narrowed to 602 results for: ACE

1. Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis

   Type

   Blog Post

   Published

   Nov. 29, 2017, 2 p.m.

   ...for Synthetic Biology and Innovation - A shared space for bringing synthetic biology to the UK with foundational...

2. MassBioEd Career Exploration Day at Addgene

   Type

   Blog Post

   Published

   Nov. 13, 2015, 3:30 p.m.

   ...comfortable surroundings” of the new Addgene office space.  Thanks Abigail, we think it’s pretty great, too...

3. Addgene's Website Update

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   Blog Post

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   April 8, 2024, 2:15 p.m.

   ...pulling all of our science options into a single place, so you can quickly navigate around the Addgene ...

4. 2022 and 2023: Reflections and Looking Forward

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   Blog Post

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   Feb. 23, 2023, 2:15 p.m.

   ...strategic refresh to understand this new world and our place in it. This period of careful reflection and planning...

5. More Data for You: Find Articles Citing Addgene Plasmids

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   Blog Post

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   June 16, 2015, 2:15 p.m.

   ...associated with the "Barcoding cells using cell-surface programmable DNA-binding domains" publication.  ...

6. How Exercise Purges Stress and Keeps Depression at Bay

   Type

   Blog Post

   Published

   Nov. 25, 2014, 4:02 p.m.

   ...studies in your own lab, you’ve come to the right place. Get started with PGC-1 plasmids from Addgene. ...

7. Keeping Up With CRISPR/Cas9

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   Blog Post

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   March 11, 2014, 5:55 p.m.

   ...technology are coming along at a fast and furious pace. In December, as we've blogged about before, there...

8. Your Top Requested Plasmid in 2016!

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   Blog Post

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   Jan. 11, 2017, 8:47 p.m.

   ...un-tagged versions of SpCas9 and further variants replace SpCas9 with SpCas9n (nickase), which nicks DNA ...

9. Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

   Type

   Blog Post

   Published

   Feb. 26, 2019, 1:40 p.m.

   ...job is to write a good, customized letter to the place that you are applying.” - Melina Fan, Chief Scientific...

10. CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping

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    Blog Post

    Published

    Nov. 29, 2018, 1:53 p.m.

    ...Doudna lab cloned the cas14 gene together with the adjacent CRISPR array and intergenic regions containing...

11. Fluorescent Proteins 101: History of Fluorescent Proteins

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    Blog Post

    Published

    Aug. 7, 2017, 1:58 p.m.

    ...part of the fluorescent protein puzzle fell into place, as they, taken alongside the insights provided ...

12. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

    Type

    Blog Post

    Published

    July 7, 2017, 1:32 p.m.

    ...and ask, "Why did I start doing this in the first place?" Many thanks to our guest, Darrell Kotton. Dr....

13. Microbes: Look, Listen, and Tell

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    Blog Post

    Published

    Aug. 28, 2018, 12:04 p.m.

    ... By connecting art and science, a fascinating interface is created that produces loads of inspiration ...

14. Developing Transferable Skills During Science Training

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    Blog Post

    Published

    June 3, 2014, 2:14 p.m.

    ... Emily Walsh The Hard Truth About Soft Skills—Workplace Lessons Smart People Wish They’d Learned Sooner...

15. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type

    Blog Post

    Published

    Jan. 23, 2014, 3:23 p.m.

    ...efficiency, simply stopped working. They finally traced the problem back to the endonuclease. “It’s just...

16. Our Most Popular Blog Posts from 2019

    Type

    Blog Post

    Published

    Dec. 31, 2019, 2:26 p.m.

    ...and more. These resources can now be found in one place: the Educational Resources page. So let’s get to...

17. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type

    Blog Post

    Published

    March 22, 2016, 2:30 p.m.

    ...Resources on the Addgene Blog Learn How the Clon Tracer Library Has Been Used in Cancer Lineage Tracing...

18. Management for Scientists: Seeking Feedback

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    Blog Post

    Published

    Jan. 8, 2015, 2:34 p.m.

    ...conducts exceptionally well-managed meetings. Members face one another, and their conversations and gestures...

19. Top 10 Open Science Developments of 2013

    Type

    Blog Post

    Published

    Dec. 19, 2013, 3:46 p.m.

    ...movement might be going is to consider some of the places it’s been. So, as 2013 comes to a close, let’s ...

20. Phage Directory: From Phage Therapy to a Repository of Phage Information

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    Blog Post

    Published

    Jan. 10, 2019, 2:38 p.m.

    ... data and information. “We want to be the go-to place for phage information because there is so much information...

21. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type

    Blog Post

    Published

    May 17, 2016, 2:30 p.m.

    ...multiple keywords that are separated by a single space to get even more specific. For example, to show ...

22. Science as Art

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    Blog Post

    Published

    March 2, 2015, 3:13 p.m.

    ...magazine . Other smaller competitions are now taking place and gathering momentum, including in Cambridge and...

23. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type

    Blog Post

    Published

    Feb. 18, 2015, 3:09 p.m.

    ...deletion efficiency mediated by clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 nuclease system...

24. Inside Addgene's 10 Year Anniversary Gala

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    Blog Post

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    July 3, 2014, 3:48 p.m.

    ... up the Addgene family. It was great to see the faces of some of our depositing scientists who weren’t...

25. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

    Type

    Blog Post

    Published

    Dec. 17, 2013, 2:47 p.m.

    ... how to be a great Mentor.    Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring...

26. Tag Your Favorite Yeast Genes with Ease

    Type

    Blog Post

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    Nov. 19, 2013, 2:37 p.m.

    ...deposited six plasmids with a multiple cloning site in place of the epitope tag for creation of your own unique...

27. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type

    Blog Post

    Published

    June 8, 2023, 1:15 p.m.

    ...publications, software, and datasets. It’s also a great place for anyone who has used IBEX and wants to preserve...

28. What's New in CRISPR - March 2020

    Type

    Blog Post

    Published

    March 10, 2020, 1:15 p.m.

    ...inactive Cas enzymes can alter gene expression via acetylation or demethylation. Casilio DNA Methylation Editing...

29. Working 9-5: an Addgene Co-op

    Type

    Blog Post

    Published

    June 2, 2022, 2:15 p.m.

    ... 3 PM, when most meetings are scheduled to take place and employees are generally expected to be available...

30. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

    Type

    Blog Post

    Published

    March 10, 2016, 3:30 p.m.

    ...being able to find their publications in the first place. As a curator of the thousands of amazing plasmid...

31. Mapping the 4D nucleome with CRISPR/Cas9

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    Blog Post

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    Aug. 11, 2015, 2:30 p.m.

    ...4D nucleome, the organization of the nucleus in space and time, and to understanding how nuclear organization...

32. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type

    Blog Post

    Published

    Aug. 13, 2014, 3:49 p.m.

    ...Gram-positive bacteria have this chemistry in their surface proteins. It is best studied in the pili that they...

33. Antibodies 101: Fc Effector Functions

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    Blog Post

    Published

    May 18, 2023, 1:15 p.m.

    ... structure, determines isotypes, and provides a place for secondary antibodies to bind. We also know that...

34. Plasmids 101: Gene, enhancer, and promoter trapping

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    Blog Post

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    May 2, 2023, 1 p.m.

    ...the known and unknown microbial coding sequence space. eLife. (2022) 11:e67667. DOI: https://doi.org/10.7554...

35. Hot Plasmids - October 2022

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    Blog Post

    Published

    Oct. 6, 2022, 1:15 p.m.

    ...of HcKCR1 in mouse neurons. D) Membrane voltage traces of action potentials in a HcKCR1-expressing mouse...

36. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type

    Blog Post

    Published

    Feb. 16, 2021, 2:15 p.m.

    ... you’re interested in (i.e. biotechnology, pharmaceuticals, etc.) Do their jobs sound interesting? Is ...

37. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type

    Blog Post

    Published

    Feb. 1, 2018, 3:07 p.m.

    ...improved feature detection with an easy to use interface. We regularly use Snapgene for our quality control...

38. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type

    Blog Post

    Published

    Dec. 13, 2017, 3:20 p.m.

    ...can also find ReFigure on Twitter (@re_figure), Facebook, and LinkedIn. Many thanks to our guest blogger...

39. SunTag and Fluorescent Imaging

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    Blog Post

    Published

    March 28, 2017, 2:30 p.m.

    ...thereby creating a single-molecule reporter of intracellular processes. Initially, Tanenbaum et al. observed...

40. FastCloning: A Newer, Simpler Cloning Technique

    Type

    Blog Post

    Published

    Nov. 1, 2016, 2:30 p.m.

    ...products with DpnI, and allow the digestion to take place for an hour at 37°C. This should be enough time ...

41. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type

    Blog Post

    Published

    July 16, 2019, 12:59 p.m.

    ...and offers a high degree of control over time and space. Zebrafish have become a popular model organism ...

42. Some Like it Hot: Thermostable GeoCas9

    Type

    Blog Post

    Published

    Sept. 14, 2017, 12:40 p.m.

    ... AA PAM Sequence NGG CRAA (R = A or G) Spacer Length 20 nt 22 nt Temperature Range for Optimal...

43. Addgene at the ASM Microbe 2016 Conference

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    Blog Post

    Published

    July 7, 2016, 2:30 p.m.

    ...science and resource sharing and was also a great place to catch up with former colleagues! I ran into past...

44. Management for Scientists: Giving Feedback

    Type

    Blog Post

    Published

    Feb. 6, 2015, 2:46 p.m.

    ...but is necessary to be direct and clear. There is place in human discourse for indirect language, but it...

45. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type

    Blog Post

    Published

    Jan. 15, 2015, 1:50 p.m.

    ...and on social media next week, so follow us on Facebook, Twitter, or subscribe to this blog. Find more...

46. Plasmids 101: Antibiotic Resistance Genes

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    Blog Post

    Published

    Jan. 30, 2014, 3:29 p.m.

    ... more stable than Ampicillin and can be used in place of Ampicillin in most applications. Antibiotics ...

47. Viral Vectors 101: Transductions

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    Blog Post

    Published

    April 30, 2024, 1:15 p.m.

    ... cell). This virus-containing media can then be placed on whatever cells you would like to infect with...

48. Summer SciComm: Science Storytelling

    Type

    Blog Post

    Published

    Sept. 16, 2022, 1:15 p.m.

    ...why you’re doing and sharing science in the first place?   References and Resources More resources on the...

49. Antibodies 101: Epitope Availability

    Type

    Blog Post

    Published

    Jan. 11, 2022, 2 p.m.

    ... your lab. Perhaps you’re even in that mythical place where antibodies are well-organized, documented,...

50. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type

    Blog Post

    Published

    June 17, 2021, 1:17 p.m.

    ... plasmid with your gene of interest, you would replace mCherry with your gene of interest. pBLADEONLY:...