We narrowed to 444 results for: Ank

1. Important Considerations in Optogenetics Behavioral Experiments

   Type

   Blog Post

   Published

   Oct. 1, 2015, 2:30 p.m.

   ...hippocampus were only recently studied in detail thanks to optogenetics. In Goshen et al, inhibition of...

2. Data Freedom: The Expansion of Data Sharing in Research Publications

   Type

   Blog Post

   Published

   Aug. 5, 2014, 6:51 p.m.

   ...for research as well as increasing transparency. Thank you to our guest blogger! Jim Woodgett is Director...

3. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

   Type

   Blog Post

   Published

   May 5, 2020, 1:15 p.m.

   ...Addgene's COVID-19 Plasmids and Resources Many thanks to our guest bloggers Shravanti Surest from Iowa...

4. Harnessing Bacterial Toxins for Allelic Exchange

   Type

   Blog Post

   Published

   Aug. 15, 2019, 12:30 p.m.

   ...the efficiency of this versatile technique. Many thanks to our guest blogger Jacob Lazarus from Harvard...

5. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

   Type

   Blog Post

   Published

   April 9, 2019, 1:13 p.m.

   ...potential negative consequences of the tag. Many thanks to our guest blogger, Joachim Goedhart! Joachim...

6. The Fluorescent Vegetables in Aptamer Soup

   Type

   Blog Post

   Published

   Jan. 29, 2019, 2:27 p.m.

   ...PubMed Central PMCID: PMC4923196. Panchapakesan, Shanker Shyam S., et al. "Ribonucleoprotein purification...

7. Antibodies 101: Normalization and Loading Controls for Western Blots

   Type

   Blog Post

   Published

   April 8, 2025, 1:15 p.m.

   ...measurement is often more accurate as a loading control. Thanks for joining us in this exploration of western blot...

8. Fluorescent Proteins 101: When GFP lets you down

   Type

   Blog Post

   Published

   Aug. 23, 2018, 12:05 p.m.

   ...effort of numerous scientists world-wide.   Many thanks to our guest blogger, Joachim Goedhart! Joachim...

9. Replacing Paper: Tips for Choosing an Electronic lab Notebook

   Type

   Blog Post

   Published

   July 10, 2018, 1:08 p.m.

   ...the lab a serious competitive advantage.  Many thanks to our guest blogger, Tea Pavlek, Product Marketing...

10. Antibodies 101: Introduction to Immunofluorescence

    Type

    Blog Post

    Published

    Nov. 16, 2021, 2:15 p.m.

    ...all claim to target your protein of interest. Thankfully, there are a number of websites compiling antibody...

11. Quick Guide to Near-Infrared Fluorescent Proteins

    Type

    Blog Post

    Published

    Oct. 5, 2021, 1:15 p.m.

    ...choosing the perfect one for your experiment!   Thank you to our guest blogger! Daria Shcherbakova is ...

12. Filming Science Videos in the Age of Social Distancing

    Type

    Blog Post

    Published

    May 20, 2021, 1:15 p.m.

    ... but we needed someone else. Design: Jennifer thankfully volunteered, and we decided to focus on a protocol...

13. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type

    Blog Post

    Published

    Sept. 5, 2017, 1:58 p.m.

    ...Cas-blocking mutations into the HDR repair template. Many thanks to our guest bloggers Dominik Paquet and Dylan ...

14. Multiple Plasmids at a Low Price: Kits from Addgene

    Type

    Blog Post

    Published

    Sept. 14, 2016, 2:30 p.m.

    ...peptides including antibiotic efflux pumps and ankyrin seven-repeat proteins: Sjuts, Hanno, et al. "Molecular...

15. Using Phosphoserine to Study Protein Phosphorylation

    Type

    Blog Post

    Published

    June 23, 2016, 2:30 p.m.

    ...phosphoproteins’ available on the Addgene website. Many thanks to guest blogger Natalie Niemi! Natalie Niemi, ...

16. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type

    Blog Post

    Published

    March 1, 2016, 3:30 p.m.

    ...catalyze directional cloning of DNA parts that are flanked by orthogonal versions of the attB and attP sites...

17. How to Design Your gRNA for CRISPR Genome Editing

    Type

    Blog Post

    Published

    Sept. 24, 2020, 1:15 p.m.

    ...experimental design, execution, and analysis. Many thanks to our guest blogger John Doench! John Doench is...

18. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type

    Blog Post

    Published

    Jan. 16, 2025, 3:42 p.m.

    ...submit your validation data after your experiment! Thanks to Addgenies Brian O’Neill and Ina Ersing for contributing...

19. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type

    Blog Post

    Published

    April 5, 2018, 12:42 p.m.

    ...high-concentration, column-purified DNA template.   Many thanks to Channabasavaiah B. Gurumurthy, Masato Ohtsuka...

20. Technologies Enabled by NanoLuc® Luciferase

    Type

    Blog Post

    Published

    Feb. 8, 2018, 12:17 p.m.

    ...to see how you utilize them in your group! Many thanks to our guest blogger Kyle Hooper. A former Technical...

21. Antibodies 101: The Basics of Western Blotting

    Type

    Blog Post

    Published

    March 8, 2021, 2:41 p.m.

    ...system the sandwich is completely submerged in a tank filled with a transfer buffer formulated to conduct...

22. Plasmids 101: Dimers and Multimers

    Type

    Blog Post

    Published

    Jan. 10, 2023, 2:15 p.m.

    ...kb). Image generated by SnapGene software.  Thanks to increasing affordability and accessibility, ...

23. Generating Mouse Models Using CRISPR/Cas9

    Type

    Blog Post

    Published

    July 12, 2016, 2:30 p.m.

    ...much to learn from biology in all its forms! Many thanks to our guest bloggers, Wenning Qin and Haoyi Wang...

24. Plasmids 101: Restriction Cloning

    Type

    Blog Post

    Published

    Feb. 18, 2016, 3:42 p.m.

    ...completed using a single enzyme if your insert is flanked on both sides by that enzyme’s restriction sites...

25. Finding Your Perfect Job After University

    Type

    Blog Post

    Published

    Jan. 12, 2016, 3:30 p.m.

    ... to study a MSc, so I had to take out a personal bank loan. I saved money from my role in Industry and...

26. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type

    Blog Post

    Published

    Nov. 6, 2015, 3:30 p.m.

    ...sure to be another exciting year for IMP! Many thanks to our guest bloggers from the International Mentorship...

27. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type

    Blog Post

    Published

    Oct. 27, 2020, 1:15 p.m.

    ..., as well as essential control plasmids. Many thanks to our guest blogger Robert Orr! Robert Orr recently...

28. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type

    Blog Post

    Published

    Oct. 8, 2020, 1:15 p.m.

    ...cautious rather than learning it the hard way! Many thanks to guest blogger Kaustubh Kishor Jadhav! Kaustubh...

29. Plasmids 101: Using Transposons in the Lab

    Type

    Blog Post

    Published

    July 6, 2021, 1:28 p.m.

    ...mini-transposon that contains the DNA cargo of interest flanked by ~50-150 bp transposon end sequences (Fig. 3A...

30. A Guide to Getting Started in Undergrad Research

    Type

    Blog Post

    Published

    June 8, 2021, 1:15 p.m.

    ... connect you with people I know who can help! Thank you to our guest blogger! Eleanor Wang is currently...

31. Important Considerations When Using AAVs

    Type

    Blog Post

    Published

    June 13, 2017, 2:30 p.m.

    ...for the scientific and medical communities. Many thanks to our guest blogger Katrina Armstrong. Katrina...

32. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type

    Blog Post

    Published

    March 7, 2017, 3:30 p.m.

    ...difficult part of the assay was designing primers flanking my edits in genomic DNA and doing PCR on genomic...

33. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type

    Blog Post

    Published

    Dec. 17, 2020, 1:30 p.m.

    ...allowing rapid progress in a wide range of fields.   Thank you to our guest blogger! Jordan Ward is an assistant...

34. Plasmids 101: Modular Cloning Applications and Kits

    Type

    Blog Post

    Published

    May 14, 2024, 1:15 p.m.

    ...written by Susanna Stroik and Meghan Rego, with thanks to Rob Hurt for his expertise and input.  References...

35. Custom CRISPR Screens & the Green Listed Software

    Type

    Blog Post

    Published

    July 11, 2017, 2:30 p.m.

    ...as inhibitory and activating approaches.  Many thanks to our guest blogger Fredrik Wermeling! Fredrik...

36. Cancer and the Immune System: Deciphering the Relationship

    Type

    Blog Post

    Published

    March 14, 2017, 2:30 p.m.

    ...of healthcare as dynamic as cancer itself. Many thanks to our guest blogger Subhadra Jayaraman! Subhadra...

37. Antibodies 101: Introduction to Antibodies

    Type

    Blog Post

    Published

    Jan. 19, 2021, 2:15 p.m.

    ... antibodies, the Developmental Studies Hybridoma Bank Learn more about nanobodies Watch our Antibodies...

38. Which Fluorescence Microscopy Techniques is Best for Me?

    Type

    Blog Post

    Published

    Oct. 10, 2017, 1:57 p.m.

    ...when heading into a new set of experiments. Many thanks to our guest blogger, Doug Richardson! Doug Richardson...

39. Special Delivery: Fluorophore Targeting for FRET Studies

    Type

    Blog Post

    Published

    July 19, 2016, 2:30 p.m.

    ...an example see Mahalingam et al., 2014).  Many thanks to our guest blogger James D. Fessenden! James ...

40. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type

    Blog Post

    Published

    Nov. 6, 2018, 1:12 p.m.

    ...reprogramming is more versatile and universal.  Many thanks to our guest blogger, Kusumika (Kushi) Mukherjee...

41. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type

    Blog Post

    Published

    Sept. 3, 2020, 12:15 p.m.

    ...By fusing the two pdDronpa domains to the sites flanking the DNA-binding cleft of Cas9, the fluorescent...

42. Technical Design of a Western Blot

    Type

    Blog Post

    Published

    Aug. 22, 2024, 1:15 p.m.

    ...reagents Good luck and happy blotting! Many thanks to Addgenies Ashley Waldron, Meghan Rego, and Amrita...

43. Troubleshooting and Optimizing a Western Blot

    Type

    Blog Post

    Published

    Sept. 17, 2024, 1:15 p.m.

    ...  References and resources References Luo, H., Rankin, G., Straley, S., & Chen, Y. (2011). Prolonged ...

44. 22 Hot Plasmid Technologies from 2014

    Type

    Blog Post

    Published

    Jan. 6, 2015, 5:21 p.m.

    ...can engineer unique enzyme recognition sites that flank a DNA module in an inverse orientation, so that ...