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We narrowed to 610 results for: CaS;

Showing: 401 - 450 of 610 results

  1. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...research being published on our own topic. Only on occasion do we then have time to read about research from...video abstracts in science journals: An overview and case study." Journal of Librarianship and Scholarly Communication...

  2. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...Addgene relied on Sanger sequencing for QC. We occasionally still use Sanger sequencing to confirm an insert...are highly repetitive, such as those containing cassettes of the same gene multiple times.  Bioinformatics...

  3. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...the first time. They can then plan accordingly in case you have questions or need a little assistance at...questions later – maybe even the next day. In the latter case, you can say “I usually need time to gather my thoughts...

  4. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...hydrogen bonds holding the DNA together, it recruits helicases to continue unwinding the strands. This creates...present in the chromosomal DNA ensure that the helicases are released from the DNA once the replication...

  5. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...initially isolated. Regrettably this is not usually the case if you obtain a cell line from a colleague. Time... been directly isolated from patients. In these cases, a test known as Short Tandem Repeat (STR) profiling...

  6. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...density within a reasonable time-frame. For these cases, some strains carry an additional measure of control... plasmid contains a chloramphenicol resistance cassette for positive selection and a p15A origin of replication...

  7. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...approaches to record keeping. Top priorities in most cases include IP protection, publications and funding....site licenses might be available as well. In most cases this applies to universities. How are you currently...

  8. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...any additional work you might need to do. In most cases, I would recommend the second option for the experiment... gave me time to listen to some of my favorite podcasts. JUST GET STARTED - One Harvard Business Review...

  9. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...approach is reliable, but it’s overkill in many cases. Often, you may not need to know the exact quantity...Although this assumption is likely true in many cases, you should validate it for your particular setup...

  10. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...is that my suggestions can help you make the best case for your candidacy. A major reason that a lot of...encouraged me to publish this manual and earn some cash for my expensive habits of running shoes and single...

  11. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...to the occasion Don’t just plan to reuse a prepared talk unless it perfectly fits the occasion.  I speak...

  12. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ... will increase the power of the results. In this case, mouse samples from positive (+), negative (-) and...antibodies will be highly specific, but in some cases they may cross-react and bind to more than their...

  13. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ... film or with specific imaging devices. In most cases, the primary antibody, which binds the protein of...inconsistent levels of the loading control. In these cases, the western should be repeated.  Finally, while...

  15. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...to knock-down expression of your target or CRISPR/Cas9 to create mutations that essentially eliminate expression...gene for your protein has been mutated using CRISPR/Cas9. You check the mutation and it causes a large deletion...

  16. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    Published
    Aug. 5, 2025, 1:15 p.m.
    ...-fidelity polymerase to ensure accuracy. In this case, you would want a low-fidelity polymerase, like ...different chimeras might function better. In that case, gene shuffling might be a good option. In gene ...

  17. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...designations all refer to the same test. On some occasions, there is an experimental group being compared... being between control vs. experimental. In this case, save yourself from family-wise error and just perform...

  18. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...been infected with a microbe, to trigger immune cascades that clear these infections. Anything that generates...immune response and initiates different immune cascades. In the research setting, antibodies of different...

  19. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...antibodies together in the same color panel. In this case, make sure to compare the excitation/emission spectra... so it's better to use a brighter fluorophore in case the CD45 expression turns out to be weak. Table ...

  20. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ... This makes it possible to use a polycistronic cassette that expresses multiple reprogramming factors ...minicircle expressing a 2A peptide polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP...

  21. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ... double peak even with a single product. In that case, you’ll want to validate your primer/probe through...Higgins, 2000; Ref, Zhu, 2002), though there are cases in which it is an appropriate choice (Panina, 2018...

  22. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...ability to cross the BBB, or the PNS, while in many cases targeting the liver less. Because these systemic...being geographically constrained to the brain. In cases where it’s critical to minimize or eliminate off-target...

  23. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ...the video to find out the final four! (Note: For cases where the same name was submitted by more than one...

  24. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...knockouts using CRISPR and Wednesday will highlight some Cas9 variants. Next, we’ll look at various CRISPR applications...

  25. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...headquarters in Watertown, Massachusetts, for the occasion! Addgenies enjoying the party. There are so ...

  26. 2021 Deck the Lab Winners!

    Type
    Blog Post
    Published
    Jan. 25, 2022, 2:15 p.m.
    ... were absolutely abuzz with anticipation as they cast their ballots for their favorite submission. So ...

  27. Twenty Years of Sharing Science!

    Type
    Blog Post
    Published
    Jan. 8, 2024, 3:25 p.m.
    ...it's hard to summarize, but we wanted to mark the occasion by trying.  When we started in 2004, we were...

  28. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ... blogs, free courses, YouTube channels, books, podcasts, and communities to help people get started in...

  30. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ...lentiviral vectors, many of which are used to deliver Cas9 and other genes to mammalian cells. For this reason...

  31. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...editing. Post: Generating Mouse Models Using CRISPR/Cas9   Erik L. Snapp, Associate Professor at Albert Einstein...

  34. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...Halloween and how seriously we take costume design. In case you need more convincing, I’ve got just the proof...

  35. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    Published
    Jan. 14, 2021, 1:30 p.m.
    ...entire floor, and put together a festive video to showcase it all!           View this post on Instagram...

  36. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    Published
    Jan. 25, 2021, 5:54 p.m.
    ... the navigation menu. The new navigation menu showcases the five main topics of the blog: Plasmids CRISPR...

  38. The Addgene Antibody Data Hub

    Type
    Blog Post
    Published
    Sept. 12, 2024, 1:15 p.m.
    ...immunohistochemistry, immunocytochemistry, ELISA — but in all cases, it contains a detailed description of the experimental...

  41. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...Approaches to Presenting the Microbial World’ showcased complex connections and ideas in various visualized...

  44. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...flexibility to CRISPR editing. Using a fusion between Cas9 and reverse transcriptase and a prime editing guide...

  46. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ...Supreme Court’s decision to ban gene patents in a case against Myriad, an organization called Free the ...

  48. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...dynamics or evaluate protein turnover rate. In many cases, however, a GFP-tagged cell line or organism has...

  49. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...following the trend and encouraging departments to showcase the work of students and staff by holding scientific...

  50. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    Published
    May 8, 2014, 3:07 p.m.
    ...success in the lab, it is important to take the occasional breather and acknowledge the team’s personal ...
Showing: 401 - 450 of 610 results