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  2. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ... Another caveat of the study is the method’s sensitivity. The authors estimate that a minimum of 150-200...necessary for a detectable signal, limiting the sensitivity of the technique. One potential solution is SunTag...

  3. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...researchers around the world. We take this responsibility seriously. Every decision we make, every project... provides unmatched customer service that is consistently timely, informative, friendly, and professional...

  4. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...generally follow the same pattern described above - a sensing domain induces changes in a fluorescent signal ...compatible with your lab and experimental setup? Consider what kind of instrumentation you have available...

  5. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...a separate plasmid. This system amplifies the intensity of the fluorescent signal and enables single molecule...SunTag, they attempted single-molecule imaging deep inside the cell, in the nucleus and cytoplasm. Again, ...

  6. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ... vesicle reporters that are detectable by an inexpensive and widely available imaging platform: ultrasound... experiments because  ultrasound devices are inexpensive, widely available, and have deep tissue penetration...

  8. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...instead results in the breakage of the ester bonds responsible for the sugar backbone of the DNA helix or even...Storing DNA: temperature and longevity You should consider a couple of more things when you elute your DNA...

  9. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...a time. Just be sure there is a point person responsible for incorporating everyone’s suggestions into...timelines for experiments and manuscripts, who is responsible for which parts of the project, and, as mentioned...

  12. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...Chance to do/learn new things, diversity of responsibilities, which might include training or teaching ...Nature Biotechnology article “Managing knowledge-intensive workers”. I have added a few comments of my own...

  13. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...for your CRISPR genome engineering experiments, consider that wildtype Cas9 with optimized chimeric gRNA...screened? A4: Before starting your experiment, consider co-transfecting with GFP. This allows you to sort...

  14. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...most applications. Antibiotics vary in their sensitivitly to heat and/or light – do not add them to media...store plates/stocks wrapped in foil if a light-sensitive antibiotic like Tetracycline is used. Keep in ...

  17. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...Since the start of this crisis, we’ve been very responsive to the mood of the Addgenies — and they were ...unfortunately we believe it is best to further reduce onsite operations for the coming week. In an abundance...

  18. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...perfect fit for Addgene. She was Head of Quality at Dimension Therapeutics, which was acquired by Ultragenyx... in 2017. As one of the original employees at Dimension Therapeutics, she helped build and grow the gene...

  20. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...communication, I had the chance to carry out a comprehensive review of the literature informing the notion...science. Understanding just how the public utilizes insights gleaned from scientific research is, after all...

  21. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...find useful antibodies for your research. Please consider sharing your experiences with antibodies from .... The content of this article is solely the responsibility of the authors and does not necessarily represent...

  24. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...important as its capabilities are the practical considerations of actually using it - the device has a rechargeable...expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel, fused to mCherry to the brain ...

  25. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...our early stage.  After each pitch we made, we considered the feedback and adjusted our business model ...company) and is our money guy (CFO).  As CEO, I am responsible for internal operations and building external...

  26. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...iTOP is best suited to binary systems in which transient protein expression has a long-lasting effect, ...in the genome. Second, expression is by nature transient, which may lower nuclease toxicity or the potential...

  27. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...impact your work? What design variables need consideration? Enrich your knowledge and skills outside of...network Attend informational interviews to gain insight into the daily routine of the potential job Join...

  28. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...Although highly useful, this technique requires extensive sample processing to avoid disrupting ribosome-mRNA...protein levels were negatively correlated with GFP intensity, showing that TRICK is an accurate readout of ...

  30. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ... However, the pH changes in our solutions are transient and return to normal once the tube is opened and...restored. We have no evidence to suggest that the transient change in pH affects AAV function. Furthermore...

  31. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...computer programming skills? Other things to consider are wage, opportunity to advance, and how many...jobs are normally available (as sectors such as forensics currently have too many applicants compared to...

  32. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...site of that. 3. Stem cell science is hard, and expensive Dr. Yamanaka explained that it took 50 people ...technologies, stem cell techniques are neither fast nor inexpensive.  After talking to numerous poster presenters...

  33. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...for specific parameters: jGCaMP7s is the most sensitive responder to action potentials. For a single action... variant 1513-WPRE (105322-AAV1) GABA sensors Sensing levels of g-Amino butyric acid (GABA) - the primary...

  34. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...production, assembled AAV vector particles accumulate inside the producer cells and purification protocols often...the outside surface of the exosome or are carried inside the vesicle, which may also contain other cellular...

  36. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ... Addgene blog – outlining various important considerations when choosing a FP, but much of the primary... (PostgreSQL), that aims to eventually be a comprehensive community resource for information about fluorescent...

  37. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ... Everyone. Plan out new experiments carefully. Consider submitting a registered report, where peer review...is based on the methods, not the results. Also consider using this framework to design your future experiments...

  38. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...new institute and mentor that they may want to consider for future work placements. The chance to learn... expectations are reasonable, your reagents are onsite before you need them, and you are set up for success...

  39. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...model yeast strain and discuss why you may want to consider a yeast off the beaten path.   Yeast as a model...for your media buck - it grows at higher cell densities than most of its counterparts, yielding higher...

  40. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...fluorescent protein fusions allowed us to image R bodies inside cells, and these constructs may be useful to future... S., Schleifer, K.-H., Verni, F. & Rosati, G. Defensive extrusive ectosymbionts of Euplotidium (Ciliophora...

  41. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...CRISPR/Cas systems have a variety of enzymes responsible for processing foreign DNA as well as the RNA...historical notes CRISPR arrays in bacterial genomes consist of repeated elements separated by unique sequences...

  44. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...And there are the usual transfection drawbacks: transient expression and needing to work with a transfectable...For cloning, Zetsche et al used four oligos that consist of direct repeats and crRNA.  Similar to a jigsaw...

  45. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...monoclonal Cas9 background because you’ll have more consistent levels of Cas9 expression between experiments...pAAV-hSyn-DIO-hM4D(Gi)-mCherry Our current AAV inventory consists almost entirely of viruses expressing DREADDs ...

  47. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...the displaced strand.   Figure 1: EvolvR consists of an error-prone polymerase (PolI) that is fused...provide a better understanding of the key residues responsible for this protein-drug interaction.   The Dueber...

  48. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...selected to participate on this panel, but my consistent writing for the Addgene blog (~1-2 blog posts...social media, but how you use social media to consistently engage with others about your topic(s) of interest...

  49. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ... effects. However, these methods can be labor intensive and quite time consuming.   As Cas9/gRNA activity...several systems to do this but most are labor-intensive, require multiple fluorescent proteins, and aren...
Showing: 401 - 450 of 725 results