We narrowed to 740 results for: NSI

... It is important to identify your audience and consider their background. Social media is a powerful tool...what you are trying to convey without having to extensively use Google search. Be careful about hidden connotations...

... another level with the addition of two light-sensitive ingredients - CRY2 and CIB1 – which they borrowed...advantages of the new system: "First, the light-responsive molecules of cryptochrome 2 are the chromophores...

...delivery by lentiviral capsid-based bionanoparticle Transient expression of CRISPR/Cas9 in cells has advantages...for packaging Cas9/gRNA ribonucleoproteins for transient expression. They replaced the tetraloop of the...

...with cells that already have Cas9). Each library consists of approximately 3,000 gRNAs, as opposed to the...still offering the potential for true biological insight. Pooled and individual plasmid formats A first ...

...CRISPR-based technique to generate C->T or G->A transitions in a target sequence in live cells. This technique...endonuclease (dCas9) fused to a deaminase to make the transitions at target sequences specified by gRNAs. Caveats...

...traction. One way to consider where the open access (OA) movement might be going is to consider some of the places...

... the spine. For binders of protocols and data, consider using color coded binders and tabs (for different...to protocols in real time. You may also want to consider moving your paper spreadsheets for reagent storage...

...Foundation are used in Parkinson’s research.  The extensive resource from the Structural Genomics Consortium...Consortium enables science to determine the three dimensional structures of proteins.  The RESOLUTE Consortium...

... Another caveat of the study is the method’s sensitivity. The authors estimate that a minimum of 150-200...necessary for a detectable signal, limiting the sensitivity of the technique. One potential solution is SunTag...

...cupcakes, or maybe the lab member originally responsible for the deposit had already moved on to their...with journal articles. But we do encourage you to consider sharing them openly as soon as that preprint is...

...researchers around the world. We take this responsibility seriously. Every decision we make, every project... provides unmatched customer service that is consistently timely, informative, friendly, and professional...

...generally follow the same pattern described above - a sensing domain induces changes in a fluorescent signal ...compatible with your lab and experimental setup? Consider what kind of instrumentation you have available...

...a separate plasmid. This system amplifies the intensity of the fluorescent signal and enables single molecule...SunTag, they attempted single-molecule imaging deep inside the cell, in the nucleus and cytoplasm. Again, ...

...instead results in the breakage of the ester bonds responsible for the sugar backbone of the DNA helix or even...Storing DNA: temperature and longevity You should consider a couple of more things when you elute your DNA...

...a time. Just be sure there is a point person responsible for incorporating everyone’s suggestions into...timelines for experiments and manuscripts, who is responsible for which parts of the project, and, as mentioned...

... vesicle reporters that are detectable by an inexpensive and widely available imaging platform: ultrasound... experiments because  ultrasound devices are inexpensive, widely available, and have deep tissue penetration...

...for our quality control process because of its expansive feature library and useful tools. In this blog...proteins that are already included in Snapgene’s extensive database. Snapgene also provides easy visualization...

...descriptions often fail to capture all of the responsibilities of a role, so it helps to see how people describe...path one you could see yourself taking? If so, consider setting up an informational interview with them...

...at his University of Wisconsin lab for a summer during my PhD and is responsible for my enduring love ...

...Chance to do/learn new things, diversity of responsibilities, which might include training or teaching ...Nature Biotechnology article “Managing knowledge-intensive workers”. I have added a few comments of my own...

...most applications. Antibiotics vary in their sensitivitly to heat and/or light – do not add them to media...store plates/stocks wrapped in foil if a light-sensitive antibiotic like Tetracycline is used. Keep in ...

...factors, including splice isoforms, to allow comprehensive screening, cellular programming, and more. For...NGS-trACKable ExpRession Element. This library consists of broad host range regulatory sequences ready...

...important as its capabilities are the practical considerations of actually using it - the device has a rechargeable...expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel, fused to mCherry to the brain ...

...communication, I had the chance to carry out a comprehensive review of the literature informing the notion...science. Understanding just how the public utilizes insights gleaned from scientific research is, after all...

...find useful antibodies for your research. Please consider sharing your experiences with antibodies from .... The content of this article is solely the responsibility of the authors and does not necessarily represent...

...replacing its dimerization domain with VVD, a light-responsive domain that dimerizes upon exposure to blue light... Ventura B (2021) Engineering AraC to make it responsive to light instead of arabinose. Nat Chem Biol....

...distributions to cover costs, which can limit expansion into new services. “We appreciate NIH’s vision.... The content of this article is solely the responsibility of the authors and does not necessarily represent...

...Since the start of this crisis, we’ve been very responsive to the mood of the Addgenies — and they were ...unfortunately we believe it is best to further reduce onsite operations for the coming week. In an abundance...

...perfect fit for Addgene. She was Head of Quality at Dimension Therapeutics, which was acquired by Ultragenyx... in 2017. As one of the original employees at Dimension Therapeutics, she helped build and grow the gene...

...2019) Fast-Seq: A Simple Method for Rapid and Inexpensive Validation of Packaged Single-Stranded Adeno-... A Rapid and Robust Assay for Batch-to-Batch Consistency Evaluation of AAV Preparations. Molecular Therapy...

...interpretations. A good peer-reviewer will help spot inconsistencies in the work, missing information that would...non-native English speaker, I sometimes miss the sensitivity to judge how aggressive my sentences might sound...

...Although we were unable to deduce the mechanism responsible we still believe that these results will be beneficial... cores and laboratories alike as an easy and inexpensive method to improve AAV yields. Although we only...

...our early stage.  After each pitch we made, we considered the feedback and adjusted our business model ...company) and is our money guy (CFO).  As CEO, I am responsible for internal operations and building external...

...iTOP is best suited to binary systems in which transient protein expression has a long-lasting effect, ...in the genome. Second, expression is by nature transient, which may lower nuclease toxicity or the potential...

...impact your work? What design variables need consideration? Enrich your knowledge and skills outside of...network Attend informational interviews to gain insight into the daily routine of the potential job Join...

...new institute and mentor that they may want to consider for future work placements. The chance to learn... expectations are reasonable, your reagents are onsite before you need them, and you are set up for success...

...model yeast strain and discuss why you may want to consider a yeast off the beaten path.   Yeast as a model...for your media buck - it grows at higher cell densities than most of its counterparts, yielding higher...

...Although highly useful, this technique requires extensive sample processing to avoid disrupting ribosome-mRNA...protein levels were negatively correlated with GFP intensity, showing that TRICK is an accurate readout of ...

...sample. The second sensor is a fusion protein consisting of sfGFP fused to a His-tagged ABC transporter... to make her work understandable to people both inside and outside the scientific community. We’re excited...

... However, the pH changes in our solutions are transient and return to normal once the tube is opened and...restored. We have no evidence to suggest that the transient change in pH affects AAV function. Furthermore...

...computer programming skills? Other things to consider are wage, opportunity to advance, and how many...jobs are normally available (as sectors such as forensics currently have too many applicants compared to...

... Everyone. Plan out new experiments carefully. Consider submitting a registered report, where peer review...is based on the methods, not the results. Also consider using this framework to design your future experiments...

... Addgene blog – outlining various important considerations when choosing a FP, but much of the primary... (PostgreSQL), that aims to eventually be a comprehensive community resource for information about fluorescent...

...production, assembled AAV vector particles accumulate inside the producer cells and purification protocols often...the outside surface of the exosome or are carried inside the vesicle, which may also contain other cellular...

...manipulate neural populations in order to get new insights into functional network interactions. Combining...bacterial strain. The sensor will be transported inside a small 3D printed robot that will “swim” in the...

...for specific parameters: jGCaMP7s is the most sensitive responder to action potentials. For a single action... variant 1513-WPRE (105322-AAV1) GABA sensors Sensing levels of g-Amino butyric acid (GABA) - the primary...

...site of that. 3. Stem cell science is hard, and expensive Dr. Yamanaka explained that it took 50 people ...technologies, stem cell techniques are neither fast nor inexpensive.  After talking to numerous poster presenters...

...fluorescent protein fusions allowed us to image R bodies inside cells, and these constructs may be useful to future... S., Schleifer, K.-H., Verni, F. & Rosati, G. Defensive extrusive ectosymbionts of Euplotidium (Ciliophora...

...CRISPR/Cas systems have a variety of enzymes responsible for processing foreign DNA as well as the RNA...historical notes CRISPR arrays in bacterial genomes consist of repeated elements separated by unique sequences...

...; or by incomplete PCR, which skips the final extension step to retain products with single-stranded overhangs...assembly is cost, as T4 polymerase is much less expensive than the enzymes required for Gibson assembly....