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  3. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 7, 2017, 1:58 p.m.
    ...the Pacific Ocean, off of the coast of Washington state. Using these samples, he was able to isolate two... protein’s structure and also discovered many mutations that alter and improve its function and shift ...

  4. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ...this blog post. 9. Droplet Digital PCR for AAV Quantitation by Kate Harten DeMaio We made the switch to...blogger Shravanti Suresh who studies CRISPR at Iowa State University detailed five methods for using CRISPR...

  5. Developing Transferable Skills During Science Training

    Type
    Blog Post
    Published
    June 3, 2014, 2:14 p.m.
    ...you need to, go to ESL classes Practice your presentation skills (as much as you can), form a peer mentoring...content-useful website, write a blog Pursue speaking invitations by inviting others (be on a speaker committee...

  6. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    Published
    May 1, 2014, 8:23 p.m.
    ...pride in facilitating the exchange of not just the physical samples, but also the legal documentation that...

  7. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...players in normal biological processes and disease states. While LITE plasmids have clear application to ...mammalian endogenous transcription and epigenetic states." Nature. 500, 472–476 (22 August 2013).   , ...

  8. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...guest blogger Sean Stacey from Eastern Tennessee State University and provides a brief rundown of Sean’...phage are super cool and have considered getting a tattoo of my favorite molecular biology tool, P1 phage...

  9. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...personal experience, is a quick way to generate many mutated plasmid constructs. The second post was contributed...specificity issues, and other means of generating mutations in live cells are all discussed further in this...

  10. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    .... Check out Joanne's Reddit AMA Here they are annotated with my comments: Everyone on the team talks...complaining. They may worry that it seems like "tattling" if others are involved. They may feel they are...

  13. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ... Using the structure of CasX at eight different states of assembly from cryo-electron microscopy, the ...activation and genome editing by fusing Cas9 to a mutated human estrogen receptor. This protein fusion stays...

  14. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...wanted to ensure applicants understood what the expectations were for the intended position. After submitting...Addgene 101” series, where each team gives a presentation on what they do and how it relates to other ...

  15. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    .... The Patriarchi Lab aimed to overcome these limitations by developing and depositing two new norepinephrine... using ColabFold (Mirdita et al., 2022). B, Representative images of HEK293T cells and neurons expressing...

  17. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...different ways to succeed – as loner or gregarious;  dictator or laissez-faire; theory or practice; business-like...clear and attractive reports, being good at presentations and of course, networking and collaborating....

  18. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...the process, that you are not losing library representation.” It’s also important that you infect enough...selection. Shalem and Sanjana say they kept a high representation of library within cells and aimed for 300 cells...

  19. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...drop off the box at any location where the United States Postal Service picks up mail. NEB actually started...drop off the box at any location where the United States Postal Service picks up mail. Recycle plastic...

  20. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...biosensors track cellular characteristics like pH, redox state, and voltage across membranes. These sensors generally... experimental setup? Consider what kind of instrumentation you have available, and the conditions of your...

  21. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ...each, thereby achieving the coveted “blue flame” status. We’re proud to honor the creativity, passion, ...that we cover all of the shipping logistics and facilitate all material transfer agreements, thereby freeing...

  22. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...repaired by base excision repair, but can lead to mutations in single stranded DNA undergoing replication....endA negative, meaning the gene has been removed/mutated to neutralize the nuclease activity. For strains...

  23. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ... detect strigolactones (SLs) are costly, non quantitative or require heavy optimization. Claudia Vickers...fluorescence intensity, it provides a direct and quantitative detection of SLs. The authors also showed that...

  24. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ...preparing for my first official professional presentation.  My first time in the lab! I got to assist...should have been nervous), but I wasn’t. The presentation went along smoothly and was followed with great...

  25. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...depends on validated reagents along with clear documentation, so we were excited when Amos Bairoch from the... the “rabbit hole” of related cell lines when annotating a cell line Community submissions via email: ...

  26. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...of fast bursts, as well as slow up/down voltage states in deep tissue, in vivo. Two of their constructs...a maximum absorption of 540 nm and two-photon excitation around 1,040 nm, has larger photocurrents, and...

  27. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...at lower temps. This approach only works for facultative thermophiles (high OR medium heat loving), but...clears the way for CRISPR/Cas9 genome editing in facultative and obligate thermophiles without the need to...

  28. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...disease typically caused by a single R206H point mutation on the receptor ACVR1. ACVR1 triggers appropriate...regeneration in healthy patients. However, the R206H mutation makes ACVR1 responsive to members of the ubiquitous...

  29. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    Published
    Nov. 17, 2015, 3:30 p.m.
    ...Society was founded in 1944 with the goal of facilitating networking between chemistry students, especially...over 170 members in its first year. The society facilitates opportunities to learn about Synthetic Biology...

  30. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...interest allowing for insertion in the correct orientation. These plasmids use a novel synthetic RPBSA promoter... exciting tool for producing specific genetic mutations and gene disruptions without the use of viral ...

  31. Management for Scientists: Giving Feedback

    Type
    Blog Post
    Published
    Feb. 6, 2015, 2:46 p.m.
    ...compare with your own perception? Tell me your interpretation of my feedback to you. What are We Learning...Steven Pinker. As a manager you must be able to state your observations truthfully and in clear terms....

  32. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...the introduction of a mutation into the genome, how can cells with that mutation be selected/screened?...

  36. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...cleaving and rejoining supercoiled DNA ends to facilitate replication. The TOPO technique specifically ...Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis to Easily Generate Insertion and Deletions in...

  37. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...the protein folded, changing it from its native state to a less folded, or denatured, one. This enables... protein of interest while it’s in a transition state or specific configuration. So how do you ensure ...

  38. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...extract.    Graphical Abstract: Schematic representation of cell-free system, capable of producing fluorescent...modify up to 60% of the disease-causing point mutations. They allow single-nucleotide modifications to...

  40. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ... This tag has been used successfully for immunoprecipitation, western blot, and immunofluorescence with...has the flexibility of also being used for immunoprecipitation, purification, and western blot as monoclonal...

  41. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ... the National Academy of Sciences of the United States of America 110.39 (2013): 15521–15529. PMC. PubMed... the National Academy of Sciences of the United States of America 70.11 (1973): 3240–3244. PubMed PMID...

  42. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...requires very little hands-on labor and could facilitate the tagging of every protein in the C. elegans...other genome modifications, including targeted mutations. SEC-based systems may prove effective in other...

  44. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...cellular components ultimately leading to cell fragmentation and death (Elmore, 2007). To study apoptosis...fluorescence based reporters however have several limitations. First, FRET signals are often difficult to detect...

  46. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...translated in a specific compartment. Despite these limitations, these techniques represent powerful tools for...translation in various cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide...

  48. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...below. Normalization is a fundamental and often understated problem in multiplex NGS library preparation....seqWell, Inc. He is a sequencing technologist and computational biologist with a passion for solving critical...

  49. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...repository provides. Repositories around the world facilitate the rapid pace of SARS-CoV-2 research by removing...and SARS-CoV with implications for clinical manifestations, transmissibility, and laboratory studies of...

  50. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...Additionally, they have an IRES-Puro marker to facilitate stable cell clone selection. Expression is driven...QBI UCSF. They are particularly interested in facilitating collaborative science, and making it accessible...
Showing: 401 - 450 of 705 results