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We narrowed to 349 results for: {{{s}}}

Showing: 161 - 180 of 349 results

  3. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...PMCID: PMC2693335. Williams JA, Luke J, Langtry S, Anderson S, Hodgson CP, Carnes AE. Generic plasmid DNA ...circular DNA forms in bacteria. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1406-13. doi: 10.1073/pnas.61.4.1406...

  4. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...species of yeast can be used for protein expression, S. cerevisiae, is the most reliable and frequently used...organism in genetics and biochemistry. When using S. cerevisiae, researchers often place recombinant proteins...

  6. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...002215549804600813 Erickson, P. A., Lewis, G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical...-1  Manning, C. F., Bundros, A. M., & Trimmer, J. S. (2012). Benefits and Pitfalls of Secondary Antibodies...

  7. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...by secondary structure. It is thought that poly(A)s are used to control the cellular concentration of ... end formation. Cell. 1987. PMID: 3568131.  Hager S, Frame FM, Collins AT, Burns JE, Maitland NJ. An Internal...

  8. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...(2015): 2321-2332. 3. Contamination, Ileana S., John P. S., Basic Methods, Human Stem Cell Manual (Second...

  9. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...dentate gyrus and olfactory bulb. Proc Natl Acad Sci U S A. 2013 Mar 19;110(12):E1152-61 Pubmed PMID 23487772...73. Pubmed PMID 22681690.  8, Wickersham IR, Finke S, Conzelmann KK, Callaway EM. Retrograde neuronal tracing...

  10. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...targeted sequence at the locus of interest. SpCas9’s 3’ NGG PAM occurs frequently in GC-rich genomes, but...AT-rich loci or genomes. Cas9s from other species, like S. aureus and N. meningitidis require different PAM ...

  11. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...components, and the accessibility of your target epitope(s). To achieve these goals, researchers commonly use...//doi.org/10.1177/33.8.3894499    Im K, Mareninov S, Diaz MFP, Yong WH (2018) An Introduction to Performing...

  12. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ... https://doi.org/10.4236/jbm.2020.89011 Shimizu, S. S. (2004). Routes of administration. In H. Hedrich...

  13. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...discussing recent publications in a specific field(s). Most anyone who has spent any time in academia has...of critique and detail? Who is/are the facilitator(s) of the journal club? Where will the journal club ...

  16. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...TALENs and CRISPR. MMEJ is active during M and early S phases, when HDR is inactive, and the balance of NHEJ...still being explored. More broadly, Sakuma et al.’s use of MMEJ represents another strategy researchers...

  18. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    Published
    July 14, 2017, 2:30 p.m.
    ...Henrici, R. C., Pecen, T. J., Johnston, J. L., & Tan, S. (2017). The pPSU Plasmids for Generating DNA Molecular...

  19. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ... Jul 27, 2020. CRISPR, and specifically Cas9 from S. pyogenes (SpCas9), is truly an exceptional genome... this paper at Addgene.  3. Gootenberg, Jonathan S., et al. "Nucleic acid detection with CRISPR-Cas13a...
Showing: 161 - 180 of 349 results