We narrowed to 444 results for: Ank
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TypeBlog PostPublishedJune 3, 2025, 1:15 p.m....: Key elements (promoter, tag, polyA site) are flanked by unique restriction sites so you can swap sequences...endonuclease-reverse transcriptase uses homology arms flanking an RNA payload to insert the payload sequence ...
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PiggyBac-ing Through the Genome Editing Field
TypeBlog PostPublishedMay 31, 2016, 3:30 p.m....cost-effective and efficient manner than ever before, thanks to the development of site-specific nucleases (...the pitfalls common to other approaches. Many thanks to our Guest Bloggers Amar Singh and Mathew Goodwin... -
Finding Your Science Policy Path
TypeBlog PostPublishedSept. 22, 2020, 1:15 p.m....networking---and they’re more accessible than ever thanks to the virtual world that the pandemic has foisted.... Image used with permission from SCI. Many thanks to our guest blogger Steph Guerra, a AAAS Science... -
Deep Dive: Statistical Tests (Comparisons)
TypeBlog PostPublishedMarch 7, 2023, 2:15 p.m....calculated p values from smallest to largest and ranking them (1 being the smallest value). It calculates...significance by finding the largest p value where its rank divided by the total number of comparisons performed... -
9 tips for a successful postdoctoral experience
TypeBlog PostPublishedOct. 9, 2018, 12:21 p.m....so remember to thank them verbally and often for their love and support. Many thanks to our guest blogger... -
Transferable Skills Guide: Teamwork
TypeBlog PostPublishedNov. 28, 2017, 2 p.m....usually the products of teams: Watson, Crick, & Franklin, Meselson-Stahl, Hershey-Chase...Who was I to ...Discovery is exactly that: a myth. The original Dr. Frankenstein from Mary Shelley’s novel worked alone, but ... -
Sequencing Options for CRISPR Genotyping
TypeBlog PostPublishedOct. 4, 2016, 2:30 p.m....and Joung 2016. Many thanks to our guest blogger Søren Hough. We additionally thank Monica Sentmanat, Victor... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog PostPublishedJune 28, 2018, 3:01 p.m....and a donor DNA that contains the tag sequence flanked by homology arms (Figure 1). In the first step ...PCR genotyping (Sanger sequencing); use primers flanking the tag sequence and expect a size-shift in the... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog PostPublishedAug. 15, 2017, 1:24 p.m.... Cre/lox recombination system. A researcher can flank fluorescent proteins in a plasmid with loxP sites...Immune response dynamics are much better understood thanks to these technologies but many questions remain... -
Typing CRISPR Systems
TypeBlog PostPublishedMarch 18, 2025, 1:15 p.m....overall. Type I systems degrade large sections of DNA thanks to the dual action power of Cas3. Cas3 functions...D. B. T., Gootenberg, J. S., Abudayyeh, O. O., Franklin, B., Kellner, M. J., Joung, J., & Zhang, F. (2017... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog PostPublishedSept. 10, 2015, 2:30 p.m....essential to test the expression of your opsin. Thankfully the Deisseroth constructs coexpress a fluorescent...your optogenetics experiments. Stay tuned! Many thanks to our guest blogger Derek Simon! Derek P. Simon... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog PostPublishedDec. 20, 2017, 2:33 p.m....you reach out to experienced legal counsel. Many thanks to our guest bloggers Lauren Celano of Propel Careers...healthcare IT, boutique consulting, investment banking and venture capital areas. We place individuals... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog PostPublishedJan. 13, 2025, 9:49 p.m..... J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C., Nelson, J. W., Newby...s41551-024-01227-1 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog PostPublishedNov. 3, 2015, 3:30 p.m....should we use all tools as some kind of a meta/Frankenstein app? Or perhaps there is a clear winner, is ...communication between developers and end users. Thanks to our guest blogger Cameron MacPherson! Cameron... -
28 Hot Plasmid Technologies from 2015
TypeBlog PostPublishedDec. 23, 2015, 3:30 p.m.... with customizable N- and C-terminal modules Thanks to the lab of Tomás Santalucía, you can now take...because they are transported back to the nucleus (thanks to a NLS on these proteins). The RFP proteins, ...Biol. 2015. PubMed PMID: 26000751 Thank you for reading. You can find all of our Hot Plasmid... -
Using AAV for Neuronal Tracing
TypeBlog PostPublishedAug. 9, 2018, 1:04 p.m....Rivest, A.J., Jung, H.-Y., Kitamura, T., Suh, J., Frank, D., Kajikawa, K., Mise, N., Obata, Y., Wickersham..., W., Zhou, S., Beyer, J., Forsayeth, J., and Bankiewicz, K.S. (2013). Axonal transport of adeno-associated... -
Viral Vectors 101: Systemic Capsids
TypeBlog PostPublishedOct. 12, 2023, 1:15 p.m....from Cynthia Arokiaraj. Addgene would also like to thank Viviana Gradinaru and Tim Shay for their support...Stevenson, O., Vergara, J., Sharma, J., Zhang, Q., Franken, T. P., Smith, J., Ibrahim, L. A., Mastro, K. J... -
New Antibodies in the Lab Animation!
TypeBlog PostPublishedNov. 19, 2024, 2:15 p.m....refresher on — these common antibody applications. Thanks for watching, and we hope you found the video radd-ically... -
Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More
TypeBlog PostPublishedSept. 8, 2017, 7:52 p.m....protein. Listen to Our Segment on mScarlet Special thanks to our sound engineer/producer, Eddy Page! Edward... -
Meet Aavery, Blugene's New Virus Friend!
TypeBlog PostPublishedFeb. 13, 2020, 2:34 p.m....Blugene's new virus friend finally has a name! We thank you for your participation in suggesting names and...