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Showing: 161 - 180 of 545 results

  1. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ... Clandinin lab used SPARC to image the calcium response in the dendrites of T5 neurons by placing the ...References: del Valle Rodríguez A, Didiano D, Desplan C (2011) Power tools for gene expression and clonal...

  2. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...often driven by the AAV p5 or p19 promoters to, respectively, produce the large (Rep78 and Rep68) and small...gene which differ between the AAV serotypes are responsible for serotype-specific differences in tissue and...

  3. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...This terrible disease causes the patient to, in response to tissue damage, have excessive bone growth until...patients. However, the R206H mutation makes ACVR1 responsive to members of the ubiquitous activin ligand family...

  4. Management for Scientists: Giving Feedback

    Type
    Blog Post
    Published
    Feb. 6, 2015, 2:46 p.m.
    ...corrective feedback. A manager must be able to respond to the employee’s level of discomfort appropriately...with the manager as with the employee. This is especially true if the problems are large enough that the...

  5. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...have demonstrated skill at managing and this is especially true in science. It is assumed that if you are...Chance to do/learn new things, diversity of responsibilities, which might include training or teaching ...

  6. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ...want to choose a research question that people (especially you!) care about. Have you critically evaluated...have done recently that has been a big hit, and especially look at the new tools and methods that have been...

  8. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ...untreated and sucrose treated viral preparations.    Despite observing a positive effect on our overall yield...Although we were unable to deduce the mechanism responsible we still believe that these results will be beneficial...

  10. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ... is positively regulated by RNA or proteins, respectively. A plasmid's copy number has to do with the ... contribute as well. These considerations are especially useful to keep in mind if you are planning to...

  11. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...Since the start of this crisis, we’ve been very responsive to the mood of the Addgenies — and they were ...said CZI Head of Science, Cori Bargmann. "CZI is especially proud to support Addgene now, when their distribution...

  13. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...different aspects of manufacturing and quality, especially with regard to compliance with health authority...cancer cells under drug treatment.” Stegmeier especially finds the value in the wealth of resources on...

  16. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...outcomes of interest, and stimulate or manipulate responses through neurological means like electric stimulation... They observed increased vagus nerve firing in response to both of these stimulations. The best part? ...

  17. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ... time, the use of episome seemed appropriate, especially since the Fertility, or F-factor discovered by...scientific community. Prior to this, bacteriophage, especially lambda, was the tool of choice for molecular ...

  18. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...explore the functional differences among mutations responsible for the disease known as Alternating Hemiplegia...disorder.” In 2012, researchers identified the gene responsible for the disease as ATP1A3. The gene encodes a...

  19. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...specific parameters: jGCaMP7s is the most sensitive responder to action potentials. For a single action potential...brighter than GCaMP6s. jGCaMP7f is the fastest responding variant. jGCaMP7b exhibits the brightest resting...
Showing: 161 - 180 of 545 results