We narrowed to 553 results for: ESP
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TypeBlog PostPublishedMay 21, 2020, 1:15 p.m.... Clandinin lab used SPARC to image the calcium response in the dendrites of T5 neurons by placing the ...References: del Valle Rodríguez A, Didiano D, Desplan C (2011) Power tools for gene expression and clonal...
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Addgene's a Nonprofit? Nonprofit Awareness Day 2017
TypeBlog PostPublishedJune 5, 2017, 3:19 p.m....researchers around the world. We take this responsibility seriously. Every decision we make, every project... in the community Addgene fosters a culture of respect, collaboration, and fun Addgene believes there ... -
Fluorescent Proteins 101: Fluorescent Biosensors
TypeBlog PostPublishedMay 18, 2017, 2:30 p.m....protein that undergoes a conformational change in response to the molecule it detects. This change then generates...with luminescent and fluorescent reporters being especially popular. We’ll give you an introduction to fluorescent... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog PostPublishedSept. 15, 2016, 2:30 p.m....This terrible disease causes the patient to, in response to tissue damage, have excessive bone growth until...patients. However, the R206H mutation makes ACVR1 responsive to members of the ubiquitous activin ligand family... -
Management for Scientists: Giving Feedback
TypeBlog PostPublishedFeb. 6, 2015, 2:46 p.m....corrective feedback. A manager must be able to respond to the employee’s level of discomfort appropriately...with the manager as with the employee. This is especially true if the problems are large enough that the... -
Management for Scientists: What Makes a Good Manager Anyway?
TypeBlog PostPublishedDec. 9, 2014, 2:22 p.m....have demonstrated skill at managing and this is especially true in science. It is assumed that if you are...Chance to do/learn new things, diversity of responsibilities, which might include training or teaching ... -
Advice for Choosing a Research Project
TypeBlog PostPublishedApril 17, 2014, 2:45 p.m....want to choose a research question that people (especially you!) care about. Have you critically evaluated...have done recently that has been a big hit, and especially look at the new tools and methods that have been... -
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog PostPublishedApril 12, 2018, 1:02 p.m....such as broccoli, cabbage and kale to name a few. Despite Arabidopsis’ utility to science, and its well-known... and variation, to growth and development, and response to environment - ABRC has kits for all these topics... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog PostPublishedApril 25, 2019, 3:01 p.m....no or minimal reduction in emission readout, respectively. mNeonGreen can be used as a fusion tag for ... maxima were red-shifted (462 nm and 492 nm, respectively), they gave this a teal classification rather... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog PostPublishedDec. 13, 2018, 1:14 p.m....untreated and sucrose treated viral preparations. Despite observing a positive effect on our overall yield...Although we were unable to deduce the mechanism responsible we still believe that these results will be beneficial... -
Plasmids 101: Origin of Replication
TypeBlog PostPublishedNov. 11, 2020, 2:23 p.m.... is positively regulated by RNA or proteins, respectively. A plasmid's copy number has to do with the ... contribute as well. These considerations are especially useful to keep in mind if you are planning to... -
Safe Port in a Storm...How Addgene is Weathering the Pandemic
TypeBlog PostPublishedSept. 17, 2020, 1:15 p.m....Since the start of this crisis, we’ve been very responsive to the mood of the Addgenies — and they were ...said CZI Head of Science, Cori Bargmann. "CZI is especially proud to support Addgene now, when their distribution... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m....Schwiesow Calcineurin is a protein phosphatase that responds to rising intracellular calcium levels within ..., which in turn binds to calcineurin-dependent response elements (CDREs) and drives gene expression. Because... -
Meet the New Addgene Board Members!
TypeBlog PostPublishedApril 28, 2020, 1:14 p.m....different aspects of manufacturing and quality, especially with regard to compliance with health authority...cancer cells under drug treatment.” Stegmeier especially finds the value in the wealth of resources on... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog PostPublishedSept. 14, 2021, 1:15 p.m....more likely to carry the full plasmid. Chakiath & Esposito (2018) noticed that in Stbl3 bacterial cultures....org/10.1007/s13205-012-0070-8 Chakiath CS, Esposito D (2007) Improved recombinational stability of... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog PostPublishedAug. 31, 2023, 1 p.m....outcomes of interest, and stimulate or manipulate responses through neurological means like electric stimulation... They observed increased vagus nerve firing in response to both of these stimulations. The best part? ... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog PostPublishedJune 17, 2021, 1:17 p.m....replacing its dimerization domain with VVD, a light-responsive domain that dimerizes upon exposure to blue light... Ventura B (2021) Engineering AraC to make it responsive to light instead of arabinose. Nat Chem Biol.... -
Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog PostPublishedOct. 29, 2015, 2:30 p.m.... time, the use of episome seemed appropriate, especially since the Fertility, or F-factor discovered by...scientific community. Prior to this, bacteriophage, especially lambda, was the tool of choice for molecular ... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog PostPublishedSept. 15, 2015, 2:30 p.m....explore the functional differences among mutations responsible for the disease known as Alternating Hemiplegia...disorder.” In 2012, researchers identified the gene responsible for the disease as ATP1A3. The gene encodes a... -
Creating accessible biology activities in schools with BioBits
TypeBlog PostPublishedAug. 2, 2018, 12:56 p.m....equivalent of a “chemistry kit” for topics in biology, especially those at the molecular and cellular level. The... of this is conducive to a classroom setting, especially for a school with a limited budget. While there...