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We narrowed to 558 results for: FIE

Showing: 161 - 180 of 558 results

  1. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ... Chemogenetics has become a popular tool in the field as it provides a noninvasive method for manipulating...target activation by ligands. PSAMs themselves are modified ligand binding domains. When coupled to the ion...for clinical use. Varenicline has been further modified to create ultrapotent PSEMS (uPSEMs) that have...

  2. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...both Cre and FLP recombinases.   Figure 1 (modified from Schnutgen et al. Nat Biotech 2003): Basic... turn off one gene and activate another. In the field of mouse genetics, FLEx is currently being used ...targeted neuronal population.    Figure 2 (modified from Schnutgen et al. Nat Biotech 2003): In the...

  3. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    Published
    July 2, 2020, 12:40 p.m.
    ...off-target sites. Long-range chromatin interactions identified with the multiplexed CAPTURE 2.0 largely replicated...replicated interactions previously identified with the original CAPTURE method. Both CAPTURE methods outperformed...both the number of unique chromatin interactions identified and the level of on-target enrichment. 2. Determining...

  4. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...presentation with people inside and outside your field. Your colleagues, lab mates, and PI can provide ...technical presentation, but those outside of your field can point out places where you gloss over essential...to be well-read on the recent literature in your field. Take your time: We often get so nervous when asked...

  5. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...expressed, one-copy ‘housekeeping’ gene), and quantified relative to a 'normal' sample with known copy...reference sequence and the gene of interest to be amplified in the same reaction. Taqman’s increased sensitivity...to confirm negative samples and, if unexpected, verified by running the product on a gel. And only ddPCR...

  6. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...12 different capsids, or serotypes, have been identified in nature, while many more have been engineered...tissues. Created with BioRender.com. Beyond the identified serotypes, AAV also has psuedotypes. Psuedotyping...., Corey, D. P., Aryee, M. J., Joung, J. K., Breakefield, X. O., Maguire, C. A., & György, B. (2019). ...

  7. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...Evan, et al. 1985). Myc was one of the earliest identified oncogenes and was a target of intense research...hoped that their new monoclonals would help the field explore Myc’s role in cell proliferation and tumorigenesis...and Pelham learned of these new antibodies and identified Myc1-9E10 and its target peptide as a suitable...

  8. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...Figure 1). Briefly, packaged DNA is extracted from purified, DNase-treated AAV and submitted for NGS. Raw ...cases, the source of the contamination can be identified, and we will discard any AAV prep we suspect ...plasmid. Our recombination software looks for and quantifies the presence of recombinants in recombinase-dependent...

  9. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...gRNA is defined by the user, and it can be easily modified to target Cas9 to virtually any genomic locus,.... Figure 2: The CRISPR library must be amplified (A) to be used to generate lentivirus (B). Cas9... Mutant cells are screened (D) and "hits" are identified using next-generation sequencing (E). Considerations...

  10. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ..., you can learn more about how nanobodies are identified in this review. If you just want to use the nanobodies...cleaved by the bdNEDP1 protease, thus releasing purified nanobodies from the column. bdNEDP1 protease can... using plasmid pDG02583. Label the Nanobodies: Purified nanobodies are then tagged with a fluorescent ...

  11. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...that your antibody is produced, harvested, purified, quantified, and validated, it’s ready for use! You ...PMC3725456. Schwarz H, Zhang Y, Zhan C, Malm M, Field R, Turner R, Sellick C, Varley P, Rockberg J, Chotteau...

  12. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...relationship between the genetic dependencies they identified and their associated ancestries. While there ...there were some exciting relationships identified, 48% were found to be artificial, so that didn't seem a ... they found that for approximately 40% of the identified genes, the genes were not expressed at all. This...

  13. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...lab expertise, experience in different scientific fields, and knowledge of how to troubleshoot problems ...product under conditions different from those specified on its data sheet?” or “Do you have additional...excited to hear about novel developments in the fields of gene and cellular therapies.     Additional ...

  14. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...scientists monitor an animal’s movement in an open field box or a circular corridor apparatus using a camera...fear. Such a behavior can be monitored in an open field test (Alpar et al., 2018). Locomotion can also be...Cre-dependent expression of  Channelrhodopsin 2 (ChR2), a modified receptor protein that activates neurons upon light...

  15. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    Published
    Aug. 13, 2019, 12:58 p.m.
    ...celebrated by the Nobel prize committee). But as the field of genetics progresses and genome sequences become...gene deactivation method in zebrafish are short, modified oligonucleotides called morpholinos. When injected... in developmental biology, and advances in this field and others are likely to continue with help from...

  16. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...script block of the Nextflow process.  Next, we specified the input and output channels for the initial ...basis, but for rapid testing and refinements, we specified a select small number of input file names or filtered...created trimmed FASTQ files as output, which we specified as input to the subsequent bbmerge process.   ...

  17. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...Addgene distributes to per year, 2005-2023. We identified and offered a number of solutions that increased...consistently accessible, wasn’t an option. So we identified countries that would benefit from a third-party...On individual material pages, we added curation fields for purpose, promoters, expression types, and more...

  18. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...dual-enrichment and mass spectrometry workflow identifies proteins bearing both labels (which had therefore...locations. C) Enrichment of dual-labeled proteins identifies proteins that had been trafficked from the “source...collection since we began distribution and has been verified to perform well in standard IHC and western blot...

  19. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...our primary target audience – colleagues in our field. Secondary audiences that may also benefit, such...quickly synthesize information from a variety of fields in order to provide useful solutions to complex... of 865,995 views for the 926 video abstracts identified. The journal Cell had the largest average view...

  20. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ... Cell culture is a widespread tool used in the fields of oncology, virology, immunology, microbiology...this reason. Unreproducible results are rife. Misidentified or cross-contaminated cells have been known ... database listing all cross contaminated or misidentified cell lines. The solution Fortunately it has ...
Showing: 161 - 180 of 558 results