...both Cre and FLP recombinases.   Figure 1 (modified from Schnutgen et al. Nat Biotech 2003): Basic... turn off one gene and activate another. In the field of mouse genetics, FLEx is currently being used ...targeted neuronal population.    Figure 2 (modified from Schnutgen et al. Nat Biotech 2003): In the...

...12 different capsids, or serotypes, have been identified in nature, while many more have been engineered...tissues. Created with BioRender.com. Beyond the identified serotypes, AAV also has psuedotypes. Psuedotyping...., Corey, D. P., Aryee, M. J., Joung, J. K., Breakefield, X. O., Maguire, C. A., & György, B. (2019). ...

...Figure 1). Briefly, packaged DNA is extracted from purified, DNase-treated AAV and submitted for NGS. Raw ...cases, the source of the contamination can be identified, and we will discard any AAV prep we suspect ...plasmid. Our recombination software looks for and quantifies the presence of recombinants in recombinase-dependent...

...off-target sites. Long-range chromatin interactions identified with the multiplexed CAPTURE 2.0 largely replicated...replicated interactions previously identified with the original CAPTURE method. Both CAPTURE methods outperformed...both the number of unique chromatin interactions identified and the level of on-target enrichment. 2. Determining...

...presentation with people inside and outside your field. Your colleagues, lab mates, and PI can provide ...technical presentation, but those outside of your field can point out places where you gloss over essential...to be well-read on the recent literature in your field. Take your time: We often get so nervous when asked...

...expressed, one-copy ‘housekeeping’ gene), and quantified relative to a 'normal' sample with known copy...reference sequence and the gene of interest to be amplified in the same reaction. Taqman’s increased sensitivity...to confirm negative samples and, if unexpected, verified by running the product on a gel. And only ddPCR...

...Evan, et al. 1985). Myc was one of the earliest identified oncogenes and was a target of intense research...hoped that their new monoclonals would help the field explore Myc’s role in cell proliferation and tumorigenesis...and Pelham learned of these new antibodies and identified Myc1-9E10 and its target peptide as a suitable...

...gRNA is defined by the user, and it can be easily modified to target Cas9 to virtually any genomic locus,.... Figure 2: The CRISPR library must be amplified (A) to be used to generate lentivirus (B). Cas9... Mutant cells are screened (D) and "hits" are identified using next-generation sequencing (E). Considerations...

...that your antibody is produced, harvested, purified, quantified, and validated, it’s ready for use! You ...PMC3725456. Schwarz H, Zhang Y, Zhan C, Malm M, Field R, Turner R, Sellick C, Varley P, Rockberg J, Chotteau...

..., you can learn more about how nanobodies are identified in this review. If you just want to use the nanobodies...cleaved by the bdNEDP1 protease, thus releasing purified nanobodies from the column. bdNEDP1 protease can... using plasmid pDG02583. Label the Nanobodies: Purified nanobodies are then tagged with a fluorescent ...

...relationship between the genetic dependencies they identified and their associated ancestries. While there ...there were some exciting relationships identified, 48% were found to be artificial, so that didn't seem a ... they found that for approximately 40% of the identified genes, the genes were not expressed at all. This...

...Addgene distributes to per year, 2005-2023. We identified and offered a number of solutions that increased...consistently accessible, wasn’t an option. So we identified countries that would benefit from a third-party...On individual material pages, we added curation fields for purpose, promoters, expression types, and more...

...lab expertise, experience in different scientific fields, and knowledge of how to troubleshoot problems ...product under conditions different from those specified on its data sheet?” or “Do you have additional...excited to hear about novel developments in the fields of gene and cellular therapies.     Additional ...

...scientists monitor an animal’s movement in an open field box or a circular corridor apparatus using a camera...fear. Such a behavior can be monitored in an open field test (Alpar et al., 2018). Locomotion can also be...Cre-dependent expression of  Channelrhodopsin 2 (ChR2), a modified receptor protein that activates neurons upon light...

...celebrated by the Nobel prize committee). But as the field of genetics progresses and genome sequences become...gene deactivation method in zebrafish are short, modified oligonucleotides called morpholinos. When injected... in developmental biology, and advances in this field and others are likely to continue with help from...

...script block of the Nextflow process.  Next, we specified the input and output channels for the initial ...basis, but for rapid testing and refinements, we specified a select small number of input file names or filtered...created trimmed FASTQ files as output, which we specified as input to the subsequent bbmerge process.   ...

...dual-enrichment and mass spectrometry workflow identifies proteins bearing both labels (which had therefore...locations. C) Enrichment of dual-labeled proteins identifies proteins that had been trafficked from the “source...collection since we began distribution and has been verified to perform well in standard IHC and western blot...

...our primary target audience – colleagues in our field. Secondary audiences that may also benefit, such...quickly synthesize information from a variety of fields in order to provide useful solutions to complex... of 865,995 views for the 926 video abstracts identified. The journal Cell had the largest average view...

... Cell culture is a widespread tool used in the fields of oncology, virology, immunology, microbiology...this reason. Unreproducible results are rife. Misidentified or cross-contaminated cells have been known ... database listing all cross contaminated or misidentified cell lines. The solution Fortunately it has ...

...Assumption #2: The loading control can be accurately quantified In western blotting, the relationship between...Assumption #2: The loading control can be accurately quantified Most housekeeping proteins are highly expressed...Assumption #2: The loading control can be accurately quantified Because the total protein is measured in a separate...