Skip to main content
Addgene
Showing: 161 - 180 of 398 results

  1. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...information you need to choose well. What are their qualifications? Do they have certifications? How can they ...posted by Toronto-based Jennifer Polk of From PhD to Life. She runs great tweet chats, has an outstanding ...

  3. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...wishing there was a machine that could make your life a hundred times easier. We’ve certainly felt this...Additional Resources on the Addgene Blog Apps for Lab Life Developing Lab Management Software for Biology Tips...

  4. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ... utilize an array of tools that rely on DNA amplification and manipulation to produce and edit plasmids...places where DNA is being created, such as PCR amplification or bacterial growth. Created with BioRender....

  7. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...between cells.      Tang et al., eLife 2020. https://doi.org/10.7554/eLife.61080     A new tool-kit for...

  8. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...past 2 years (Alberts et al, PNAS 2014; Taylor, eLife, 2016). Several Future of Research (FoR) symposia...al. “Avoiding a lost generation of scientists.” eLife 5 (2016): e17393. Pubmed PMID: 27175853. Pubmed ...

  9. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...virus-based experiment at one point or another. After amplifying a viral vector, collecting virus, and transducing...replication. To avoid genomic rearrangements, try amplifying the viral vector with bacteria designed to minimize...

  10. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...processing and contributes to variability in RNA half-life, and ultimately gene-expression. Polyadenylation...with reduced viral titer, but improved transcript life, and so should be handled with care (4). For this...

  11. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...changed our whole outlook on the possibilities in life for employment?  It never happened – there was never...moment”. But there were a lot of very small steps. Life in a cutting-edge synthetic biology lab Keira and...

  12. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...Chaokun Li and colleagues. Here you simply PCR-amplify your vector and your insert, DpnI digest away the...1. Li, Chaokun, et al. "FastCloning: a highly simplified, purification-free, sequence-and ligation-independent...

  13. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ... P0), infecting new Sf9 cells, and getting an amplified P2 virus. This P2 virus can be produced in a greater...Baculovirus replicates inside insect cells, and its lifecycle ends in cell lysis, releasing the virus to the...

  14. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...Andrew York, Maria Ingaramo, and their team at Calico Life Sciences recently set out to do just that — by developing...Timmel, C. R., & Hore, P. J. (2016). Chemical amplification of magnetic field effects relevant to avian ...

  17. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ... those questions in this post as we explore… the life and times of the myc tag.  Origins of protein tags...would help the field explore Myc’s role in cell proliferation and tumorigenesis.  Using two different synthetic...

  19. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...Resources I asked some colleagues to share valuable life lessons they have learned during their scientific...wrote about them in a blog post on Born to Science: Life Lessons from Scientific Training More and more ...

  20. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...required by MMEJ can easily be added via PCR amplification. Given the inefficiency of HDR for knock-ins...Select for puromycin resistant cells Step 5: PCR amplify and sequence the locus to verify correct GFP-puro...
Showing: 161 - 180 of 398 results