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...etc.) can effect reprogramming rates and such a number maybe misleading. Integrating iPSC delivery methods...robust and efficient generation of iPSCs is the number one priority, with safety taking lower priority...

...you run the plate.    Analyzing There are a number of methods available for analyzing qPCR data, most...Shaun S. LightCycler qPCR optimisation for low copy number target DNA. Journal of Immunological Methods 270...

... effective at generating plasmids with varying numbers of repeated sequences or inserts, which can be ...authors optimized these systems by: 1) decreasing the number of plasmids required for each input/output system...new origin of replication increases plasmid copy number in E. coli. The authors used pXPG to study the ...

...undesirable bacteria. This system can be delivered a number of ways but success has been observed with Phagemids... host range plasmid that can be maintained in a number of bacteria native to the honey bee and bumble ...

...to your application — and double-check that lot number while you’re at it! Polyclonal or monoclonal Both...you are running a denaturing blot.  There are a number of lysing methods and buffers to choose from. If...

... on the addgene.org homepage! You can track the number of plasmids right below the search bar and more...

...open-source distributors such as Addgene, as well as a number of online user forums such as http://www.genome-engineering.org...

...adjustments for the type of tissue culture dish and number of dishes used. Here’s to making AAV packaging ...

...plasmid sharing over the years, we crunched the numbers (shout out to Addgenie Priyanka Ramanathan for ...

...Allied Sciences in Ghana. He’s done research at a number of institutions in Ghana and most recently got ...

...downstream effects including possibly decreasing the number of postdocs various academic institutions can afford...

...decide what lab to join? 11:25 - 11:50 : What is the number one question you think rotation students should...

...amino acid immediately followed by its position (number) relative to the start Methionine (Met) followed...

...progress their research. *2015 total reflects the number of distributed plasmids as of March 26, 2015. How...

...symptoms. The World Health Organization says the number of new cases could rise to 10,000 a week within...

...mutations in the rat APOBEC1 that decreases the number of off-target RNA edits by >390 fold and >3,800...

...potential and the promise of science to help a great number of people. I look forward to working with Addgene...

...epitope tags like FLAG, HA, and Myc can adopt a number of different conformations and thus potentially...

...realize its potential as a research tool. Soon, the number of people studying it grew. In particular, studies...

...possible to quickly and efficiently assemble a large number of building blocks with just two or three inexpensive...