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We narrowed to 610 results for: REV

Showing: 161 - 180 of 610 results

  1. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ...Annals of Internal Medicine published systematic reviews on an agent used to promote bone growth based on... open access as it does about the state of peer review. 8)     That same issue of Science also included...showing overwhelming support for open access to peer-reviewed journal publications, although many don’t necessarily...

  2. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    Published
    May 8, 2014, 3:07 p.m.
    ...recent conversations with three lab heads have revealed that a combination of hard work, determination...How do you manage work-life balance? Tom Ellis reveals “I don't. Ask me again in five years and hopefully...Scientists Maintain a Good Work-Life Balance? Read the Previous Posts in this Series Advice for Choosing a Research...

  3. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    Published
    July 20, 2017, 2:30 p.m.
    ... et al. "PP09. 1–2352: Systematic review of incidence and prevalence of spinal muscular atrophy (SMA)....S65. Additional Resources on the Addgene Blog Previous BeHeard Awardees Other Efforts to Support Rare...

  4. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...be completed and reviewed. The timeline therefore will be as follows:  Request review: 1–2 days Prep and...µL (5 mL).  Once submitted, the request will be reviewed by Addgene for legal approvals and technical compatibility...

  5. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...Utility. Annual Review of Pharmacology and Toxicology 55:399–417 . https://doi.org/10.1146/annurev-pharmtox-...Controlling Stimulation Some studies rely on delivering reversible or varied amounts of stimulation to receptors...

  6. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    Published
    Dec. 13, 2017, 3:20 p.m.
    ...analyses with the relevant figures from a literature review. No more hunting through a whole folder of papers...might include a confirmatory image to show that a previously used reagent performs well, or you might publish... one such research product which can convince reviewers of your expertise and insight. We recommend sharing...

  7. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    Published
    June 24, 2025, 1:15 p.m.
    ...you’re ready. Plus, what better addition to peer review than having your plasmids sequenced and openly ...moved on to their next position. We do routinely review deposits that are left in the hold status and reach...depositing plasmids with Addgene! We'll continue to review our policies to make sure they meet our goals, ...

  8. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ...sequence reverse complement tool: Enter a DNA sequence and the program will give you the reverse sequence...sequence, complement sequence, or reverse and complement sequence.  Filter DNA tool: Removes non-DNA characters...

  9. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ... Need a quick review of the different immune responses? We got you! You can also review antibodies in ...Antibodies can bind to a pathogen and physically prevent it from entering a cell, a non-Fc-dependent effector...

  10. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ... a single point mutation in the PAM sequence, preventing the Cas nucleases from re-identifying it (3)..... Addgene has empowered researchers to harness previous experimental successes and further develop the... FJM, Wolf YI, Yakunin JvdO, Koonin EV. Nature Reviews Microbiology. 2011 Jun; 9, 467-477. Bondy-Denomy...

  11. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    Published
    June 17, 2021, 1:17 p.m.
    ...spatio-temporal regulation, and is difficult to reverse, making it unsuitable for some types of experiments...to do is co-transform pBLADEONLY. That way, a previously cloned gene of interest, under the pBAD promoter...confined experiments as well as those requiring reversibility that you cannot do with AraC,” says Di Ventura...

  12. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    Published
    April 23, 2020, 1:15 p.m.
    ...standard AAV QC regimen does not address this.  Previous studies have demonstrated that NGS is an effective...tagmentation preparation approaches described previously require a preliminary double stranding step. ...specific signature sequences in the data. Unlike previously described thermodynamics-based methods of serotype...

  13. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...species and applications. In this blog, we will review the most common tags, the antibodies used to detect...optimizations and specific monoclonal antibodies. Below we review the most commonly available tags and their antibodies...Myc was developed in 1985 and is still the most prevalent clone used today! Goat, chicken, mouse, or rabbit...

  14. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...metabolites in Escherichia coli or Saccharomyces cerevisiae with few techniques that are easily transferrable...oft published metabolic engineering realm of S. cerevisiae and E. coli. The utility of simultaneous and... of this to control a metabolic network in S. cerevisiae by using three RNA programs to differentially...

  15. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...with the Harvard Business Review or some Chartered Institute of Marketing reviews, I would have struggled...problem was I knew I had a knowledge gap. I had no previous business, marketing or sales training and knew...

  16. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...introductions to potential publications or prepare a review article. These are both options that can be done... started early on introductions and literature reviews could make the months leading up to your defense...Consider submitting a registered report, where peer review is based on the methods, not the results. Also ...

  17. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...enabling everyone to participate in the biotech revolution. Our lab exists in what appears to be a funky...scientists Anyone can become a member of BosLab and previous laboratory experience is not required. Our members...microbiome project. This project was inspired by previous city-wide genomic studies and our study will involve...

  18. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...activation. Normally, β10-11 are antiparallel. To prevent binding, the β strands in FlipGFP were redesigned...fluorescence within 2 to 5 hours, a time consistent with previous methods. In zebrafish, the Shu Lab used FlipGFP...PMCID: PMC3790201. Elmore, Susan. "Apoptosis: a review of programmed cell death." Toxicologic pathology...

  19. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...Using this road map, and what was known about previously characterized TFs, the Zhang group were able ...most immediately useful for TF library screens. Previously engineered TF libraries were not as comprehensive...transformation and amplification, refer to the Zhang lab’s previously published protocol and its relevant modifications...

  20. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ... is conserved in all living organisms and has previously been used for multiplex Cas9-nuclease editing...existing multiplex array architectures, including previous tRNA-gRNA arrays, that rely on an upstream lengthy..._VT (#186716).   Fig. 2: Comparison of previous representative dual-gRNA architectures with DAP...
Showing: 161 - 180 of 610 results