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We narrowed to 468 results for: SEC

Showing: 161 - 180 of 468 results

  2. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...spectrum disorder. It is estimated that CTD is the second cause of male intellectual disability after Fragile...leaflets, instead of three) and acute aortic dissections (a tear in the inner layer of the aorta) are ...

  3. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ... if there is either success or failure: In our second example we’ve added some simple but critical logic...procedures, not artificially constraining them). In the second example we have a separate worm model and tube ...

  5. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ... as effective in mentoring for this transition. Second, a coach will help you set goals and then hold ...us) offers a free report on her website called “Secrets to Success in Graduate School”. She has also launched...

  6. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...with NRs) exist in steady-state complexes with secondary coregulator (or co-coregulator) partners. The ...information can be found in the Datasets and Reagents sections of the current NURSA website. NURSA and Addgene...

  7. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ... piece on mStayGold inspired and informed this section.  Find mStayGold plasmids at Addgene here!   Ando...  Neurogenetic alchemy: CAP-Mac unleashes the secrets of primate brains with brilliance By Hridaya Patel...

  8. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...cargo-specific safety   After replication competency, the second concern with virus safety is the disease-risk of...ultracentrifugation, heat sealed tubes can be used for extra security. Finally, when spinning down lentivirus, you’ll...

  9. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...of the new viral preps from recent months: New INTRSECT viral preps available!  We are expanding our inventory...ready to use viral preps from the Deisseroth lab INTRSECT project. Check out the new additions.  Image calcium...

  10. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...and mVenus (bottom). Cells were imaged every 30 seconds for 3 hours. Image from Lee et al., 2020. SPOTlight....A184S-AAV1 here New Dual and Triple feature INTRSECT viral preps from the Deisseroth Lab now available...

  11. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...easy sample transfer between 96 well plates. The second pod can pick up 8 tips and position the tips indepently... tricks, be sure to share them in the comments section.   Additional Resources on the Addgene Blog Apps...

  14. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...one end of the DNA fragment (Figure 1, middle). A second recombination event then excises the DNA between...invert the reporter and GeneR fragment. Then the second recombination event would excise the reporter from...

  15. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ... of a bigger movement in a variety of research sectors to provide open source technologies and spaces ...bacterial species in the gut of the domesticated housecat, and seeing how that makeup differs from that ...

  16. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...in what is called a reeling mechanism (see next section for details). The newly formed single-stranded ...that the two breakpoints will repair without the section between the two gRNAs, creating a deletion. However...

  17. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ..., and nanobody. For more information read our secondary nanobodies blog post.  A rapid selection platform...RANbodies: Reporter Nanobody Fusions Plasmids 101: Secondary Nanobody Toolbox Resources on Addgene.org View...

  18. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...target sequence for U6 promoter expression and the second site accepts the homology arm repair template. ...fusion of the desired gRNA to the U6 promoter, and a second plasmid containing the homology arms and an N- ...

  19. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...making their surrounding media acidic through the secretion of lactic acid (a by-product of cellular metabolism...John P. S., Basic Methods, Human Stem Cell Manual (Second  Edition), 2012. Additional Resources on the Addgene...

  20. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ... reads should align to this reference sequence. Second, we perform a megaBLAST search on all the reads...vectors for your research. Please use the comments section below to let us know if you have any specific questions...
Showing: 161 - 180 of 468 results