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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 385 results for: abo.5

Showing: 161 - 180 of 385 results

  1. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ... Therapy Vol. 25 No 5. PubMed PMID: 28391961. PubMed Central PMCID: PMC5417792. 5. Naldini,L., 2015. Gene...famously visited Congress to help increase awareness about the potential of gene therapy!). It would take another...muscular atrophy, neurodegenerative diseases and metabolic diseases are also underway and reported results...

  2. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...luciferase protein interact and enable intein splicing (5). Screening with yeast two hybrid systems While initial... Nature. 1989; 340:245-246. PubMed PMID: 2547163. 5. Ozawa, T., Kaihara, A., Sato, M., Tachihara, K., ... Plasmids Read our Blog Post About Yeast Vectors Read our Blog Post About Tagging Yeast Genes ...

  3. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...an attL-containing entry vector.   Figure 5: Method C to create an entry clone: Restriction cloning... Hosts. Curr Protoc Protein Sci. 2003 Feb;Chapter 5:Unit 5.17. PubMed PMID:18429245. 3. Ptashne, M. (1992... expression systems. Keep reading to learn more about the Gateway cloning method and its advantages. An... on the Addgene Blog Try Out FastCloning Learn About Methylation and Restriction Enzymes Use Colony PCR...

  4. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...recommended for use in immunohistochemistry (Figure 5). Anti-MAPT [AT8] specifically recognizes the disease-associated...to the Addgene Antibody Data Hub.  Figure 5: Immunohistochemistry with Addgene's Anti-APP [6E10...synaptic events By Brian O’Neill The Lin Lab and collaborators recently created ASAP5, a faster and more responsive...

  5. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ... users select some of the best designs (e.g., top 5) and test them in their labs. Figure 2: Guidelines...high generalization performance. Science Advances, 5(11), eaax9249. https://doi.org/10.1126/sciadv.aax9249... al., 2023). We trained our models in close collaboration with the Krauthammer lab (University of Zurich...

  6. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...microscopy with sequential multiple-view reconstruction (5). In order to image large tissue such as cleared rodent... shadows appear as stripes in the acquired image (5).  A way to get around this problem is to illuminate...protein (GFP) (6).   The Dodt and Frank Bradke laboratories later developed the 3DISCO (3-dimensional imaging...

  7. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    Published
    Jan. 23, 2018, 2:04 p.m.
    ...Plasmid : 1 pHelper: 1 RepCap OR 3 Transfer Plasmid : 5 pHelper: 2 RepCap Select what type of tissue culture...packaging experiments. Let us know what you think about the calculator in the comments section below. References...

  8. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...PAM sequence for recognition by QCascade; although 5’-CC-3’ is one of the best PAMs for VchINT, many other...by QCascade (Fig. 1B). The process also creates a 5-bp target-site duplication (TSD), a hallmark characteristic..., 2020), to multi-copy genomic integration of metabolic cassettes (Zhang et al. 2020); however, the technology...

  9. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...Edelhoch method. Int J Biol Macromol. 2015;77:260-5. doi: 10.1016/j.ijbiomac.2015.03.027. Epub 2015 Mar... in Colloid & Interface Science, Volume 19, Issue 5, 2014, Pages 438-449, ISSN 1359-0294, https://doi....validated, it’s ready for use! You can learn more about common antibody applications in our Antibodies 101...

  10. Editor's Choice, October 2016

    Type
    Blog Post
    Published
    Nov. 4, 2016, 2:30 p.m.
    ... for Grant Writing 5 Reasons to Use Reddit for Science Communication Changing Labor Laws Bring Increased...record readership with over 75,000 views. Changing Labor Laws Bring Increased Postdoc Wages Our most popular... for anyone considering a postdoc. In “Changing Labor Laws Bring Increased Postdoc Wages,” Future of Research...thorough yet concise rundown of how recently enacted labor laws will likely increase postdoc earnings in the...changes quickly and harmlessly. Learn much more about the laws and their possible repercussions in Gary...This post leads readers to our latest podcast all about graduate student life and how establishing a thriving... labor laws...

  11. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...e-commerce company and establishing credibility Chapter 5 - Realizing that Addgene would be successful and the...repository’s future. If you’ve ever been curious about what it takes to start your own nonprofit, this ...Harvard University, where she studied diabetes and metabolism. Melina has worked at Genetics Institute in Cambridge...

  12. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    Published
    May 2, 2024, 1:15 p.m.
    ... facilitate the isolation of modified cells; and (5) is compatible with the introduction of unique molecular...faithfully model the complex spatial, cellular, metabolic, and cytokine microenvironment that exists in ...LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested in...

  13. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ...Retrograde (AAVrg), and volumes of 1 mL, 2.5 mL, or 5 mL. Please note that all orders will be shipped in...your browsing and requesting. If you’re curious about the service, read on.  Expanding our viral vector...

  14. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...whose plans are unknown (up to 33% from 22%), a full 5% of postdocs who were expecting a salary increase ...Previous Article: Changing Labor Laws Bring Increased Postdoc Wages Read about the Advocating for Science... of $47,476 per year, under updates to the Fair Labor Standards Act (FLSA). This was due to the threshold...postdocs - and in some cases, faculty - have found out about what their institution is doing from Future of Research...Science Symposium and Workshop Learn about a Variety of Science Careers Resources on Addgene.org Find Career...

  15. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...reserved for questions. 5. Understand the job you are applying for It's not about you and how you will finally...members to discuss your research plans for the next 5-10 years. You will discuss the focus of your first... potential colleagues study. Think about how you might collaborate or, at the absolute minimum, can appreciate...the first sentence or two what your research is about and what you think you can accomplish. No matter...your seminar accessible. Let the experts ask you about the details and then you can display your deep knowledge...expert's question than to leave everyone confused about your research because you waded too deep into the...simple in principle The first question I usually get about chalk talks is, what is a Chalk Talk? It is a 60...

  16. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...place I could be happy with it. Cycle 5 - The Future! Figure 5: The mobile monitor and teleprompter... Production Specialist, I’m constantly thinking about ways to improve our productions to deliver the best...four months after I started with Addgene, I was about to have a check-in with my boss Jennifer. I had ... would be a couple months. Jennifer and I spent about an hour that morning scrambling. Everything we had...someone to be comfortable on camera while thinking about things like lighting, camera angle, and shot composition... allowing people to sit without having to worry about getting in close to mic them up. Monitor: I then...first video in our Intro to the Lab Bench series about PPE! We made this with a combination of in-lab filming...

  17. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...Elements of Acidithiobacillia. The CRISPR Journal, 4(5), 656–672. https://doi.org/10.1089/crispr.2021.0051...720–725. https://doi.org/10.1038/s41586-021-03886-5 Pinilla-Redondo, R., Mayo-Muñoz, D., Russel, J., Garrett...Cleavage during Type III CRISPR-Cas Immunity. Cell, 161(5), 1164–1174. https://doi.org/10.1016/j.cell.2015.04.027...Class 1 is the most abundant of the two, comprising about 90% of identified CRISPR-Cas in bacteria and nearly...CRISPR systems called CasPEDIA, with information about enzyme activity, experimental conditions, and more...

  18. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...creativity. Gallery Books. Boyes, A. (2019, November 13). 5 mental mistakes that kill your productivity. Harvard...Retrieved January 14, 2022, from https://hbr.org/2019/11/5-mental-mistakes-that-kill-your-productivity Finkelstein...brainstorming sessions.  Allow the group time to think about the problem before you meet. This holds the dual... meetings run smoother when people have thought about the issue ahead of time. Encourage dissent. The ... decisions while encouraging the group to think about alternative options. When a devil’s advocate is ...a few minutes every month dedicated to thinking about the future will improve your ability to innovate...to be able to continue our experiments. Thinking about these plans before you’ve run out of consumables...

  19. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ... different part types (e.g. 5’-GGAG and 3’-TACT flanking promoter parts, 5’-AATG and 3’-GCTT flanking ...Robert Hurt and Mohamed Soufi. So you’ve learned about the Modular Cloning (MoClo) system and what it can...these would be interchangeable between different laboratories. Therefore, choosing acceptor vectors and parts...Created with BioRender.com. The Sainsbury Laboratory Golden Gate Cloning videos made by the Nicola...system, are an invaluable resource for learning about MoClo in an in-depth and easy-to-understand guided... from Addgene will most likely arrive in your laboratory as plates of frozen glycerol stocks. It is very...

  20. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...consider the following example gel. Figure 5: An example gel (left) with bands corresponding to...of western blots. Expert Review of Proteomics, 11(5), 549–560. https://doi.org/10.1586/14789450.2014.939635... this chain (blue) to infer or calculate things about earlier ones. In practice, multiple sources of error...condition 2.” By eye, the loading control appears about the same between the two conditions. But it isn’.... However, it throws both assumptions discussed above into question. Assumption #1: The loading control...searching the literature to see what is already known about your housekeeping protein in your context. If you...step from the POI blot, you don’t need to worry about the linear detection ranges of multiple targets....
Showing: 161 - 180 of 385 results