...digest as depicted in Lane 2. Controlling methylation Finally, you can control methylation by altering your...

...use attP sequence docking sites so that you can control exactly in which locus your transgene ends up (...selection markers include vermillion (another gene controlling eye color) and yellow (a gene affecting the body...

...that expresses gene III. Gene III is under the control of a T7 RNA polymerase and cannot be expressed ...the deaminase TadA-7.10 A mutagenesis plasmid controlling the phage mutation rate Phage infection results...

...lox system with optogenetics. Cre activity is controlled by red light by splitting Cre and fusing each...system, cells express various fluorescent proteins controlled by Cre recombination. Each cell can have multiple...

...communicating the results of the work. Yes, those control experiments are important - but without letting...and finish that experiment (and don’t forget the controls!). And when you’re ready to write up the paper...

...maintained within the yeast cell. These elements control not only the number of plasmids found in each cell... The copy number of these vectors can also be controlled if specific regulatable elements are included...

...overcome by using a Tet-inducible virus to allow for controlled expression of reprogramming factors. Check out...the presence of transgenes is still an important control. Additionally, Sendai virus doesn’t rely on promoters...that multiple transfection makes it difficult to control the dose of plasmid the cells receive over the ...Ohtaka, and Mahito Nakanishi. "Novel Strategy to Control Transgene Expression Mediated by a Sendai Virus-Based...

... in more detail in part 2 of this blog series) controls the cell types in which the conditional recombination...engineered mouse model allows gene expression to be controlled through a drug or small molecule that regulates...

...individual proteins under endogenous regulatory control. But even with the advent of CRISPR/Cas9 technology... in human cell lines. Furthermore, the quality control necessary to ensure the edited cells are behaving...

...virus and its infectivity in check. Engineering controls, safety systems that are built into how something...functions, were designed for this purpose. One such control is dividing up the essential components of a virus...

...and serotype selection. Promoters and serotype control, respectively, cell-specific gene expression and...viral infection. Cre or Flp dependent switches control gene expression via switches placed in your cell...

...technologies utilize chemical modifications to the RNA to control the entire editing system with a simple LED light...al. Photoswitchable gRNAs for Spatiotemporally Controlled CRSIPR-Cas-based Genomic Regulation. ACS 6, 5...

...Addgene gave to me: ways to use optogenetics to control CRISPR editing.  On the fifth day of CRISPR, Addgene...

...site or circuit can occur. Promoters The promoter controls how the gene of interest will be expressed; it...et al., 2002). They are useful when you need to control the timing of expression in your experiment. Some... much virus to put into your system, which is controlled through titer (concentration, given as viral ...

...detection. You’ll also want to use the appropriate controls for your experiment to account for potential non-specific...quantitatively if you also load the gel with appropriate controls for normalizing the protein concentration. To ...plasmid encoding GFP. Actin, a common internal control for Western blots, shows that a similar concentration...

...expression in C.elegans. In this system a promoter controls the expression of a GAL-4 driver that can bind...split intein for refined spatiotemporal transgene control in Caenorhabditis elegans." Proceedings of the ...

...lines Treat parental cells (or cells expressing a control -- for example, an FKBP12F36V-tagged LACZ) with...: dTAG-7 and dTAG-13 Erb et al. Transcription control by the ENL YEATS domain in acute leukemia. Nature...

... of MET/Met genes from the pYES2.1 plasmid is controlled by the GAL1 promoter. The cloning procedure adds...absence of methionine. Since the GAL1 promoter controls MET/Met gene expression, complementation should...

...blue light.  Similarly, light can also be used to control homo-oligomerization of a protein fused to CRY2...coli. This operon is under the transcriptional control of a strong constitutive promoter (BBa_J23100) ...

...infect target tissues. Improved specificity through control of gene expression and the selection of specific...CRISPR-AAV vectors Learn about Addgene AAV Quality Control Process Resources on Addgene.org Find ready-to-use...