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We narrowed to 389 results for: control

Showing: 161 - 180 of 389 results

  2. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...The gRNAs targeting EGFP can be used as positive controls for gene editing or gene activation, while the...from your primary goal. Getting these quality-controlled reagents from Addgene can greatly expedite a ...

  7. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...communicating the results of the work. Yes, those control experiments are important — but without letting...and finish that experiment (and don’t forget the controls!). And when you’re ready to write up the paper...

  8. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...maintained within the yeast cell. These elements control not only the number of plasmids found in each cell... The copy number of these vectors can also be controlled if specific regulatable elements are included...

  9. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...overcome by using a Tet-inducible virus to allow for controlled expression of reprogramming factors. Check out...the presence of transgenes is still an important control. Additionally, Sendai virus doesn’t rely on promoters...that multiple transfection makes it difficult to control the dose of plasmid the cells receive over the ...Ohtaka, and Mahito Nakanishi. "Novel Strategy to Control Transgene Expression Mediated by a Sendai Virus-Based...

  10. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...virus and its infectivity in check. Engineering controls, safety systems that are built into how something...functions, were designed for this purpose. One such control is dividing up the essential components of a virus...

  11. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...and serotype selection. Promoters and serotype control, respectively, cell-specific gene expression and...viral infection. Cre or Flp dependent switches control gene expression via switches placed in your cell...

  12. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...individual proteins under endogenous regulatory control. But even with the advent of CRISPR/Cas9 technology... in human cell lines. Furthermore, the quality control necessary to ensure the edited cells are behaving...

  13. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ... in more detail in part 2 of this blog series) controls the cell types in which the conditional recombination...engineered mouse model allows gene expression to be controlled through a drug or small molecule that regulates...

  14. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...technologies utilize chemical modifications to the RNA to control the entire editing system with a simple LED light...al. Photoswitchable gRNAs for Spatiotemporally Controlled CRSIPR-Cas-based Genomic Regulation. ACS 6, 5...

  15. The Twelve Days of CRISPR

    Type
    Blog Post
    Published
    Dec. 22, 2020, 2:15 p.m.
    ...Addgene gave to me: ways to use optogenetics to control CRISPR editing.  On the fifth day of CRISPR, Addgene...

  16. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...site or circuit can occur. Promoters The promoter controls how the gene of interest will be expressed; it...et al., 2002). They are useful when you need to control the timing of expression in your experiment. Some... much virus to put into your system, which is controlled through titer (concentration, given as viral ...

  17. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...detection. You’ll also want to use the appropriate controls for your experiment to account for potential non-specific...quantitatively if you also load the gel with appropriate controls for normalizing the protein concentration. To ...plasmid encoding GFP. Actin, a common internal control for Western blots, shows that a similar concentration...

  18. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...lines Treat parental cells (or cells expressing a control -- for example, an FKBP12F36V-tagged LACZ) with...: dTAG-7 and dTAG-13 Erb et al. Transcription control by the ENL YEATS domain in acute leukemia. Nature...
Showing: 161 - 180 of 389 results