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Showing: 161 - 180 of 419 results

  1. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ... (budding yeast), S. pombe (fission yeast), A. thaliana (mustard weed), O. cuniculus (rabbit), synthetic... plasmid is intended to be used. Choose from: mammalian expression, bacterial expression, lentiviral, ...

  4. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...educational background, cultural or religious affiliation, and ethnic or personal identity. We do this ...dialogue, a major part of our everyday work is to liaise with different organizations and individuals, to...

  6. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...are a common model organism used to understand mammalian traits and genetically engineered mouse models...series, I’ll highlight terminology you should be familiar with before working with mouse models, several...

  7. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...j.molcel.2018.03.031 Xiao, Y., Luo, M., Dolan, A. E., Liao, M., & Ke, A. (2018). Structure basis for RNA-guided... B. W., Nyer, D. B., Kennedy, R., Banh, L. M., Williams, C., Toh, M. S., Irby, M. J., Edwards, L. S., ...

  8. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...FR=France; KR=South Korea; CH=Switzerland; AU=Australia.  It probably comes as no surprise that the...plasmids. But many other countries, from Japan to Australia, are significant depositors and requestors. CRISPR...

  9. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...beyond the tag and small molecule. It does work in mammalian cells, mice, and even yeast, though (we have a...rapid and complete degradation of proteins in mammalian cells. Proc Natl Acad Sci U S A. 2012 Dec 4;109...

  10. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...plasmids used to inactivate specific genes in mammalian cells. Despite the popularity of CRISPR-based ...Genes On and Off Resources on Addgene.org Find Mammalian Expression Vectors Find Cre-Lox Plasmids Learn...

  11. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...genomic loci in other model systems, including mammalian cells. CRISPaint (CRISPR-assisted insertion ...fluorescently tag markers of cellular structures in mammalian cells. These plasmids use fluorescent proteins...

  12. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ... dangerous, non-physiological temperature for mammalian cells and one which diminishes the endonuclease...can indeed be optimized for genome editing in mammalian cells. However, the fact that most of the researchers...

  13. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...pCMMP-MCS-IRES-Puro IRES Retroviral pEF1a-IRES-Neo IRES Mammalian MSCV-IRES-Luciferase IRES Retroviral pWPI ...pWPI IRES Lentiviral AmCyan-P2A-mCherry P2A Mammalian pC5Kan-P2A P2A Insect pUltra P2A and T2A Lentiviral...

  14. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...with hundreds of variants available. Read on to familiarize yourself with the available FP emission colors...optimized for 37°C, and is therefore most suited for mammalian or bacteria studies, whereas GFPS65T is better...

  15. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ... Starting material: plant, microbe, blood, or mammalian cells or tissue Product: total nucleic acids While...purify DNA and RNA from plants, microbes, blood or mammalian cells in 30 seconds or less without using a pipette...

  16. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...prevalent or life-threatening (cystic fibrosis, hemophilia, muscular dystrophy)? AAV gene therapy started...trials for devastating genetic diseases such as hemophilia, muscular dystrophy, spinal muscular atrophy,...

  17. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...efficiency through automation.       More Resource Avaliable at Addgene Get help with plasmid transformation... molecular biology reference pages! Resources Avaliable on the Addgene Blog Read more Inside Addgene Posts...

  18. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...precise, efficient A•T-to-C•G base editing in mammalian cells and embryos. Nature Biotechnology, 42(4)...editors for efficient T-to-G and C-to-G editing in mammalian cells. Nature Biotechnology, 42(10), 1538–1547...

  19. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ... transcription by blocking its initiation. In mammalian cells, robust repression requires targeting dCas9...delivery systems can be found here “CRISPR 101: Mammalian Expression Systems and Delivery Methods”. Validating...

  20. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...lentiviral expression of the human KRAS gene in mammalian cells. STEP 1: Generate an Entry Clone There are...expression level, and experimental purpose. For mammalian lentiviral expression, we could use a vector like...
Showing: 161 - 180 of 419 results